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Toll-Like Receptor 9 Antibody (YA3321)

(Synonyms: Toll-Like Receptor 9; CD289; TLR9; Toll like receptor 9; Toll like receptor 9 isoform A precursor; Toll like receptor 9 isoform B)
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Based on 1 Customer Validation

Toll-Like Receptor 9 Antibody (YA3321) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Toll-Like Receptor 9.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in rabbit IgG in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Toll-Like Receptor 9 Antibody (YA3321)
  • Toll-Like Receptor 9 Antibody (YA3321) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Toll-Like Receptor 9 Antibody (YA3321) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Toll-Like Receptor 9.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 130 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 116 kDa
Species Reactivity Database

Entrez Gene: 54106 Human

SwissProt: Q9NR96 Human

Immunogen

A synthesized peptide derived from human TLR9 aa400-550/1032.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105684

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Toll-Like Receptor 9 Antibody (YA3321)
    Western blot analysis was performed on extracts from Raji (lane 1, 20 μg), and Ramos (lane 2, 20 μg) using Toll-Like Receptor 9 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Toll-Like Receptor 9 is key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Controls lymphocyte response to Helicobacter infection. Upon CpG stimulation, induces B-cell proliferation, activation, survival and antibody production[1][2][3][4].

  • Subcellular Localization

    Endoplasmic reticulum membrane; Single-pass type I membrane protein; Early endosome membrane; Lysosome; Cytoplasmic vesicle, phagosome; Golgi apparatus membrane

  • Expression


    Tissue_specificity:This gene is highly expressed in the spleen, lymph nodes, tonsils, and peripheral blood leukocytes, especially in plasmacytoid anterior dendritic cells. Expression levels are much lower in monocytes and CD11c+ immature dendritic cells. It has also been detected in the lungs and liver.

  • Isoforms & Post-Translational Modification

    Q9NR96 has 5 isomers: Q9NR96-1: 115860 Da (predicted); Q9NR96-2: 109628 Da (predicted); Q9NR96-3: 118517 Da (predicted); Q9NR96-4: 118652 Da (predicted); Q9NR96-5: 114237 Da (predicted).
    Activated by proteolytic cleavage of the flexible loop between repeats LRR14 and LRR15 within the ectodomain. Cleavage requires UNC93B1. Proteolytically processed by first removing the majority of the ectodomain by either asparagine endopeptidase (AEP) or a cathepsin followed by a trimming event that is solely cathepsin mediated and required for optimal receptor signaling;Palmitoylated by ZDHHC3 in the Golgi regulates TLR9 trafficking from the Golgi to endosomes. Depalmitoylation by PPT1 controls the release of TLR9 from UNC93B1 in endosomes

  • Subunit

    Monomer and homodimer. Exists as a monomer in the absence of unmethylated cytidine-phosphate-guanosine (CpG) ligand.

  • SwissProt ID

    Q9NR96

  • Gene ID
    54106 [NCBI]
  • Synonyms

    Toll-Like Receptor 9; CD289; TLR9; Toll like receptor 9; Toll like receptor 9 isoform A precursor; Toll like receptor 9 isoform B

  • Research Field

    Immunology

Toll-Like Receptor 9 Antibody (YA3321) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Raji (lane 1, 20 μg), and Ramos (lane 2, 20 μg) using Toll-Like Receptor 9 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Toll-Like Receptor 9 Antibody (YA3321) Product Image
    1/16
  • Experimental Validation Results for Toll-Like Receptor 9 Antibody (YA3321)
  • Toll-Like Receptor 9 Antibody (YA3321) Product Image
  • Experimental Validation Results for Toll-Like Receptor 9 Antibody (YA3321)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Raji (lane 1, 20 μg), and Ramos (lane 2, 20 μg) using Toll-Like Receptor 9 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Toll-Like Receptor 9 Antibody (YA3321)
  • Toll-Like Receptor 9 Antibody (YA3321) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Raji (lane 1, 20 μg), and Ramos (lane 2, 20 μg) using Toll-Like Receptor 9 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Toll-Like Receptor 9 Antibody (YA3321) Product Image WB