Claudin 18.2 Antibody

製品番号: HY-P81773: データシート SDS COA User Guide for Antibodies
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Claudin 18.2 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Claudin 18.2.

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容量	価格（税別）	在庫状況	数量
無料サンプル		今すぐ申し込む
10 μL	$75	在庫あり	Estimated Time of Arrival: December 31
50 μL	$200	在庫あり	Estimated Time of Arrival: December 31
100 μL	$325	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明

製品説明

Claudin 18.2 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Claudin 18.2.

Host

Rabbit

Clonality

Polyclonal

分子量

Predicted band size: 28 kDa;

Observed band size: 35 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P56856

Gene ID

51208 [NCBI]

Immunogen

Synthetic peptide corresponding to N terminal of Human CLDN18 (P56856).

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:500
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	純度	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

ALL IHC-P FC

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using Claudin 18.2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using Claudin 18.2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Flow cytometric analysis of 1X10^6 F9 cells labeling Claudin 18.2 Antibody (HY-P81773, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

Function：Involved in alveolar fluid homeostasis via regulation of alveolar epithelial tight junction composition and therefore ion transport and solute permeability, potentially via downstream regulation of the actin cytoskeleton organization and beta-2-adrenergic signaling (By similarity). Required for lung alveolarization and maintenance of the paracellular alveolar epithelial barrier (By similarity). Acts to maintain epithelial progenitor cell proliferation and organ size, via regulation of YAP1 localization away from the nucleus and thereby restriction of YAP1 target gene transcription (By similarity). Acts as a negative regulator of RANKL-induced osteoclast differentiation, potentially via relocation of TJP2/ZO-2 away from the nucleus, subsequently involved in bone resorption in response to calcium deficiency (By similarity). Mediates the osteoprotective effects of estrogen, potentially via acting downstream of estrogen signaling independently of RANKL signaling pathways (By similarity); Involved in the maintenance of homeostasis of the alveolar microenvironment via regulation of pH and subsequent T-cell activation in the alveolar space, is therefore indirectly involved in limiting C.neoformans infection; Required for the formation of the gastric paracellular barrier via its role in tight junction formation, thereby involved in the response to gastric acidification

Subcellular Localization：Cell junction, tight junction; Cell membrane; Multi-pass membrane protein; Cell junction, tight junction; Cell junction, tight junction; Lateral cell membrane

Expression：
Tissue_specificity:The expression of this gene is limited to the lungs; the expression of this gene is limited to the gastric mucosa, mainly found in the epithelial cells of the gastric pits and the epithelial cells of the base of the gastric glands, including exocrine and endocrine cells (at the protein level) .

Isoforms & Post-Translational Modification：P56856 has 2 isomers: P56856-1: 27856 Da (predicted); P56856-2: 27720 Da (predicted).

Subunit：Interacts with TJP2/ZO-2 (By similarity). Interacts with TJP1/ZO-1 (By similarity).

RRID

AB_3103878

Database

Entrez Gene: 51208 Human ; 56492 Mouse ;

SwissProt: P56856 Human ; P56856-2 Human ; P56857 Mouse ;

OMIM: 609210 Human

Research Field

Cell Biology

Synonyms

CLDN 18.2,Claudin 18.2

ドキュメンテーション

Select Batch:

データシート (235 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)