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MCM5 Antibody (YA9790)

(Synonyms: CDC46, MCM5, MGC5315, P1 CDC46)
Customer Review

Based on 1 Customer Validation

MCM5 Antibody (YA9790) is a Rabbit-derived and non-conjugated IgG Monoclonal antibody, targeting to MCM5.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS, Glycerol and BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MCM5 Antibody (YA9790)
  • MCM5 Antibody (YA9790) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1000 1:50-100 1:50

Product Details

Description

MCM5 Antibody (YA9790) is a Rabbit-derived and non-conjugated IgG Monoclonal antibody, targeting to MCM5.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 82 kDa; Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 82 kDa
Immunogen

Synthetic peptide of human MCM5

Sensitivity

Endogenous

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, Glycerol and BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MCM5 Antibody (YA9790)
    Western blot analysis was performed on protein extracts (25 μg) from Ramos (lane 2), Jurkat (lane 3), U87 (lane 4), and 293T(lane 5) using MCM5 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    MCM5 acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity[1][2][3][4][5][6].

  • Subcellular Localization

    Nucleus; Chromosome

  • Isoforms & Post-Translational Modification

    MCM5 has an amino acid length of 734, molecular weight is 82286 Da.
    UFMylated at Lys-583; UFMylation occurs at the ongoing replication fork and promotes the CMG replicative DNA helicase complex formation.

  • Subunit

    Component of the MCM2-7 complex.

  • SwissProt ID

    P33992

  • Synonyms

    CDC46, MCM5, MGC5315, P1 CDC46

References

[1]. Li Z, et al. MCM5 UFMylation regulates replication origin firing and fork progression. EMBO J. 2025 Nov;44(21):6019-6050. [Content Brief]

[2]. Tsuji T, et al. Essential role of phosphorylation of MCM2 by Cdc7/Dbf4 in the initiation of DNA replication in mammalian cells. Mol Biol Cell. 2006 Oct;17(10):4459-72. [Content Brief]

[3]. Rzechorzek NJ, et al. CryoEM structures of human CMG-ATPγS-DNA and CMG-AND-1 complexes. Nucleic Acids Res. 2020 Jul 9;48(12):6980-6995. [Content Brief]

[4]. Jones ML, et al. Structure of a human replisome shows the organisation and interactions of a DNA replication machine. EMBO J. 2021 Dec 1;40(23):e108819. [Content Brief]

[5]. Jenkyn-Bedford M, et al. A conserved mechanism for regulating replisome disassembly in eukaryotes. Nature. 2021 Dec;600(7890):743-747. [Content Brief]

[6]. Baris Y, et al. Fast and efficient DNA replication with purified human proteins. Nature. 2022 Jun;606(7912):204-210. [Content Brief]

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MCM5 Antibody (YA9790) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Ramos (lane 2), Jurkat (lane 3), U87 (lane 4), and 293T(lane 5) using MCM5 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • MCM5 Antibody (YA9790) Product Image
    1/16
  • Experimental Validation Results for MCM5 Antibody (YA9790)
  • MCM5 Antibody (YA9790) Product Image
  • Experimental Validation Results for MCM5 Antibody (YA9790)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Ramos (lane 2), Jurkat (lane 3), U87 (lane 4), and 293T(lane 5) using MCM5 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for MCM5 Antibody (YA9790)
  • MCM5 Antibody (YA9790) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from Ramos (lane 2), Jurkat (lane 3), U87 (lane 4), and 293T(lane 5) using MCM5 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • MCM5 Antibody (YA9790) Product Image WB