Complement C5 Antibody (YA6533)

Cat. No.: HY-P86840: Data Sheet COA
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User Guide for Antibodies
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Complement C5 Antibody (YA6533) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Complement C5.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Complement C5 Antibody (YA6533) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Complement C5.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 188 kDa;

Observed band size: 188/72 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P01031

Gene ID

727 [NCBI]

Immunogen

Recombinant protein within human C5 aa 1-900 / 1,676.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200
FC FC: Flow Cytometry	1:1000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Precursor of the C5a anaphylatoxin and complement C5b components of the complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed:12878586, PubMed:18204047, PubMed:30643019, PubMed:6554279). Activated downstream of classical, alternative, lectin and GZMK complement pathways (PubMed:12878586, PubMed:18204047, PubMed:30643019, PubMed:6554279)|Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis (PubMed:26841837, PubMed:27052168, PubMed:30552328, PubMed:30643019). Complement C5b is generated following cleavage by C5 convertase and initiates formation of the MAC complex: C5b binds sequentially C6, C7, C8 and multiple copies of the pore-forming subunit C9 (PubMed:30552328, PubMed:30643019). During MAC complex assembly, the C5b6 subcomplex, composed of complement C5b and C6, associates with the outer leaflet of target cell membrane, reducing the energy for membrane bending (PubMed:30552328, PubMed:32569291)|Mediator of local inflammatory process released following cleavage by C5 convertase (PubMed:8182049, PubMed:9553099). Acts by binding to its receptor (C5AR1 or C5AR2), activating G protein-coupled receptor signaling and inducing a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes (PubMed:8182049, PubMed:9553099). C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation (PubMed:8182049, PubMed:9553099)

Subcellular Localization：Secreted,Secreted,Target cell membrane,Secreted

Isoforms & Post-Translational Modification：P01031: 1676 amino acids, molecular weight 188305 Da.

Subunit：In absence of complement activation, the C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond

Synonyms

Complement C5 C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 Complement C5 beta chain Complement C5 alpha chain C5a anaphylatoxin Complement C5 alpha' chain C5 CPAMD4

Documentation

Select Batch:

Data Sheet (231 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)