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Sclerostin Antibody

(Synonyms: Sclerostin, Sost)
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Based on 1 Customer Validation

Sclerostin Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Sclerostin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Sclerostin Antibody
  • Sclerostin Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-2000 1:50-200

Product Details

Description

Sclerostin Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Sclerostin.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 30 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 24 kDa
Immunogen

Purified recombinant protein of human Sclerostin.

Sensitivity

Endogenous

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Sclerostin Antibody
    Western blot analysis of extracts from Mouse lung tissue (lane 2, 20ug), HepG2 (lane3, 20μg) using Sclerostin Antibody (HY-P811321). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Sclerostin is a Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation

  • Subcellular Localization

    Secreted, extracellular space, extracellular matrix

  • Expression


    Tissue_Specificity: Widely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteoblasts differentiated for 21 days. Detected in the subendothelial layer of the aortic intima (at protein level).

  • Isoforms & Post-Translational Modification

    Sclerostin has 2 isoforms, Q9BQB4-1: amino acid length is 213, molecular weight is 24031 Da (predicted); Q9BQB4-2: amino acid length is 217, molecular weight is 24264 Da (predicted).

  • Subunit

    Interacts with LRP4 (via the extracellular domain); the interaction facilitates the inhibition of Wnt signaling.

  • SwissProt ID

    Q9BQB4

  • Gene ID
    50964 [NCBI]
  • Synonyms

    Sclerostin, Sost

Sclerostin Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Mouse lung tissue (lane 2, 20ug), HepG2 (lane3, 20μg) using Sclerostin Antibody (HY-P811321). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Sclerostin Antibody Product Image
    1/16
  • Experimental Validation Results for Sclerostin Antibody
  • Sclerostin Antibody Product Image
  • Experimental Validation Results for Sclerostin Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Mouse lung tissue (lane 2, 20ug), HepG2 (lane3, 20μg) using Sclerostin Antibody (HY-P811321). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Sclerostin Antibody
  • Sclerostin Antibody Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Mouse lung tissue (lane 2, 20ug), HepG2 (lane3, 20μg) using Sclerostin Antibody (HY-P811321). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Sclerostin Antibody Product Image WB