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FDFT1 Antibody (YA3512)

(Synonyms: DGPT; ERG9; FDFT1; SQS; Squalene synthase; SS)
Customer Review

Based on 1 Customer Validation

FDFT1 Antibody (YA3512) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FDFT1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for FDFT1 Antibody (YA3512)
  • Experimental Validation Results for FDFT1 Antibody (YA3512)
  • Experimental Validation Results for FDFT1 Antibody (YA3512)
  • Experimental Validation Results for FDFT1 Antibody (YA3512)
  • Experimental Validation Results for FDFT1 Antibody (YA3512)
  • FDFT1 Antibody (YA3512) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50

Product Details

Description

FDFT1 Antibody (YA3512) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FDFT1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Calculated Molecular Weight Predicted band size: 48 kDa;Observed band size: 48 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human FDFT1 aa150-300/417.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102789

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for FDFT1 Antibody (YA3512)
    Western blot analysis of extracts from A549(lane 2, 20μg), HCT116(lane 3, 20μg), HepG2(lane 4, 20μg) and SH-SY5Y(lane 5, 20μg) using FDFT1 Antibody (HY-P81002). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FDFT1 Antibody (YA3512)
    Immunohistochemical analysis of paraffin-embedded rat liver tissue using FDFT1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for FDFT1 Antibody (YA3512)
    Immunohistochemical analysis of paraffin-embedded rat liver tissue using FDFT1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for FDFT1 Antibody (YA3512)
    Immunocytochemistry analysis of HepG2 cells labeling FDFT1 Antibody (HY-P81002) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FDFT1 Antibody (HY-P81002) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for FDFT1 Antibody (YA3512)
    Immunocytochemistry analysis of SH-SY5Y cells labeling FDFT1 Antibody (HY-P81002) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FDFT1 Antibody (HY-P81002) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Catalyzes the condensation of 2 farnesyl pyrophosphate (FPP) moieties to form squalene. Proceeds in two distinct steps. In the first half-reaction, two molecules of FPP react to form the stable presqualene diphosphate intermediate (PSQPP), with concomitant release of a proton and a molecule of inorganic diphosphate. In the second half-reaction, PSQPP undergoes heterolysis, isomerization, and reduction with NADPH or NADH to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway

  • Subcellular Localization

    Endoplasmic reticulum membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Widely expressed

  • Isoforms & Post-Translational Modification

    P37268 has 5 isomers: P37268-1: 48115 Da (predicted); P37268-2: 40770 Da (predicted); P37268-3: 38317 Da (predicted); P37268-4: 35101 Da (predicted); P37268-5: 43060 Da (predicted).

  • SwissProt ID

    P37268

  • Gene ID
    2222 [NCBI]
  • Synonyms

    DGPT; ERG9; FDFT1; SQS; Squalene synthase; SS

  • Research Field

    Cardiovascular

FDFT1 Antibody (YA3512) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549(lane 2, 20μg), HCT116(lane 3, 20μg), HepG2(lane 4, 20μg) and SH-SY5Y(lane 5, 20μg) using FDFT1 Antibody (HY-P81002). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using FDFT1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using FDFT1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling FDFT1 Antibody (HY-P81002) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FDFT1 Antibody (HY-P81002) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of SH-SY5Y cells labeling FDFT1 Antibody (HY-P81002) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FDFT1 Antibody (HY-P81002) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • FDFT1 Antibody (YA3512) Product Image
    1/16
  • Experimental Validation Results for FDFT1 Antibody (YA3512)
  • Experimental Validation Results for FDFT1 Antibody (YA3512)
  • Experimental Validation Results for FDFT1 Antibody (YA3512)
  • Experimental Validation Results for FDFT1 Antibody (YA3512)
  • Experimental Validation Results for FDFT1 Antibody (YA3512)
  • FDFT1 Antibody (YA3512) Product Image
  • Experimental Validation Results for FDFT1 Antibody (YA3512)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549(lane 2, 20μg), HCT116(lane 3, 20μg), HepG2(lane 4, 20μg) and SH-SY5Y(lane 5, 20μg) using FDFT1 Antibody (HY-P81002). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FDFT1 Antibody (YA3512)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using FDFT1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for FDFT1 Antibody (YA3512)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using FDFT1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for FDFT1 Antibody (YA3512)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling FDFT1 Antibody (HY-P81002) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FDFT1 Antibody (HY-P81002) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for FDFT1 Antibody (YA3512)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of SH-SY5Y cells labeling FDFT1 Antibody (HY-P81002) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FDFT1 Antibody (HY-P81002) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for FDFT1 Antibody (YA3512)
  • Experimental Validation Results for FDFT1 Antibody (YA3512)
  • Experimental Validation Results for FDFT1 Antibody (YA3512)
  • Experimental Validation Results for FDFT1 Antibody (YA3512)
  • Experimental Validation Results for FDFT1 Antibody (YA3512)
  • FDFT1 Antibody (YA3512) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549(lane 2, 20μg), HCT116(lane 3, 20μg), HepG2(lane 4, 20μg) and SH-SY5Y(lane 5, 20μg) using FDFT1 Antibody (HY-P81002). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.

  • FDFT1 Antibody (YA3512) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat liver tissue using FDFT1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • FDFT1 Antibody (YA3512) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat liver tissue using FDFT1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • FDFT1 Antibody (YA3512) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling FDFT1 Antibody (HY-P81002) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FDFT1 Antibody (HY-P81002) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • FDFT1 Antibody (YA3512) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of SH-SY5Y cells labeling FDFT1 Antibody (HY-P81002) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FDFT1 Antibody (HY-P81002) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • FDFT1 Antibody (YA3512) Product Image WB
  • FDFT1 Antibody (YA3512) Product Image IHC-P
  • FDFT1 Antibody (YA3512) Product Image IHC-P
  • FDFT1 Antibody (YA3512) Product Image ICC
  • FDFT1 Antibody (YA3512) Product Image ICC