PKA RI alpha Antibody (YA1751)(PBS only)

Cat. No.: HY-P82006A: Data Sheet User Guide for Antibodies
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PKA RI alpha Antibody (YA1751) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PKA RI alpha.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PKA RI alpha Antibody (YA1751) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PKA RI alpha.

Host

Rabbit

Molecular Weight

Predicted band size: 43 kDa;

Observed band size: 43 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

P10644

Gene ID

5573 [NCBI]

Immunogen

A synthetic peptide of human Protein Kinase A regulatory subunit I alpha

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells

Subcellular Localization：Cell membrane

Expression：
Tissue_specificity:Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible

Isoforms & Post-Translational Modification：P10644 has 2 isomers: P10644-1: 42982 Da (predicted); P10644-2: 38003 Da (predicted).
The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity

Subunit：The inactive holoenzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP releases the two active catalytic monomers and the regulatory dimer. Interacts with PRKACA and PRKACB (PubMed:33058759).

Research Field

Signal Transduction

Synonyms

PRKAR1A; PKR1; PRKAR1; TSE1; cAMP-dependent protein kinase type I-alpha regulatory subunit; Tissue-specific extinguisher 1; TSE1

Documentation

User Guide for Antibodies (1077 KB)