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TGFBR3 Antibody (YA4173)

(Synonyms: BGCAN; betaglycan)
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Based on 1 Customer Validation

TGFBR3 Antibody (YA4173) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to TGFBR3.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2b

  • Application:

    WB, ELISA

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for TGFBR3 Antibody (YA4173)
  • TGFBR3 Antibody (YA4173) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:10000

Product Details

Description

TGFBR3 Antibody (YA4173) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to TGFBR3.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 93 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 93 kDa
Immunogen

Purified recombinant fragment of human TGFBR3 (AA: 147-328 ) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2b

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TGFBR3 Antibody (YA4173)
    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), THP-1 (lane 3), Jurkat (lane 4), A549 (lane 5), and Mouse testis (lane 6) using TGFBR3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    TGFBR3 cell surface receptor that regulates diverse cellular processes including cell proliferation, differentiation, migration, and apoptosis. Initiates BMP, inhibin, and TGF-beta signaling pathways by interacting with different ligands including TGFB1, BMP2, BMP5, BMP7 or GDF5. Alternatively, acts as a cell surface coreceptor for BMP ligands, serving to enhance ligand binding by differentially regulating BMPR1A/ALK3 and BMPR1B/ALK6 receptor trafficking. Promotes epithelial cell adhesion, focal adhesion formation and integrin signaling during epithelial cell spreading on fibronectin. By interacting with the scaffolding protein beta-arrestin2/ARRB2, regulates migration or actin cytoskeleton and promotes the activation of CDC42 as well as the inhibition of NF-kappa-B. In gonadotrope cells, acts as an inhibin A coreceptor and regulates follicle-stimulating hormone (FSH) levels and female fertility. Plays a role in the inhibition of directed and random cell migration in epithelial cells by altering the actin cytoskeletal organization. Participates in epithelial-mesenchymal transformation (EMT) upon binding to BMP2 or TGFB2, by activating the PAR6/SMURF1/RHOA pathway; (Microbial infection) May act as a receptor for human cytomegalovirus in different cell types by interacting with HCMV trimer composed of GO, GH and GL[1][2][3][4][5][6].

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Secreted; Secreted, extracellular space, extracellular matrix

  • Isoforms & Post-Translational Modification

    Q03167 has 2 isomers: Q03167-1: 93499 Da (predicted); Q03167-2: 93428 Da (predicted).
    Extensively modified by glycosaminoglycan groups (GAG);Phosphorylated in the cytoplasmic domain by the type II receptor TGFBR2 at THR-840 to mediate recruitment of ARRB2 and subsequent internalization of TGFBR2 and TGFBR3

  • Subunit

    Forms homodimers and homooligomers (PubMed:11323414). Interacts with DYNLT4 (PubMed:16982625). Interacts with integrin ITGA5:ITGB1; this interaction promotes the internalization and trafficking of ITGA5:ITGB1 into endocytic vesicles (PubMed:22562249). Interacts with TGFB1, BMP2, BMP5, BMP7 or GDF5 and inhibin A via the ligand binding domains (PubMed:18184661). Interacts with ALK3/BMPR1A; this interaction results in the cell surface retention of BMPR1A (PubMed:19726563). Interacts with ALK6/BMPR1B; this interaction enhances BMPR1B-mediated stimulation of the BMP signaling pathway (PubMed:19726563). Interacts with the scaffolding protein beta-arrestin2/ARRB2; this interaction mediates internalization of TGFBR3 and thus regulates migration, actin cytoskeleton and activation of CDC42 (PubMed:12958365, PubMed:18184661, PubMed:19416857, PubMed:19325136)

  • SwissProt ID

    Q03167

  • Gene ID
    7049 [NCBI]
  • Synonyms

    BGCAN; betaglycan

TGFBR3 Antibody (YA4173) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), THP-1 (lane 3), Jurkat (lane 4), A549 (lane 5), and Mouse testis (lane 6) using TGFBR3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • TGFBR3 Antibody (YA4173) Product Image
    1/16
  • Experimental Validation Results for TGFBR3 Antibody (YA4173)
  • TGFBR3 Antibody (YA4173) Product Image
  • Experimental Validation Results for TGFBR3 Antibody (YA4173)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), THP-1 (lane 3), Jurkat (lane 4), A549 (lane 5), and Mouse testis (lane 6) using TGFBR3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for TGFBR3 Antibody (YA4173)
  • TGFBR3 Antibody (YA4173) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), THP-1 (lane 3), Jurkat (lane 4), A549 (lane 5), and Mouse testis (lane 6) using TGFBR3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • TGFBR3 Antibody (YA4173) Product Image WB