NCoA-3 Antibody (YA4615)

Cat. No.: HY-P84918: Data Sheet COA
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NCoA-3 Antibody (YA4615) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NCoA-3.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation
References

Description

NCoA-3 Antibody (YA4615) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NCoA-3.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 155 kDa;

Observed band size: 150-160 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9Y6Q9

Gene ID

8202 [NCBI]

Immunogen

Purified recombinant fragment of human AIB1 aa 276-398.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Solution

Formulation

Supplied in PBS with 0.05% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from K562(lane 1(40μg) ),MCF-7(lane 2(40μg) ),Hela(lane 3(40μg) ),A549(lane 4(40μg) )and Jurkat(lane 5(40μg) ) using AIB1 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit

Subcellular Localization：Cytoplasm; Nucleus

Expression：
Tissue_specificity:Widely expressed. Highly expressed in the heart, skeletal muscle, pancreas, and placenta. Lowly expressed in the brain, and extremely lowly expressed in the lungs, liver, and kidneys.

Isoforms & Post-Translational Modification：Q9Y6Q9 has 5 isomers: Q9Y6Q9-1: 155293 Da (predicted); Q9Y6Q9-2: 153797 Da (predicted); Q9Y6Q9-3: 154400 Da (predicted); Q9Y6Q9-4: 147499 Da (predicted); Q9Y6Q9-5: 154892 Da (predicted).
Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function;Methylated by CARM1;Phosphorylated by IKK complex. Regulated its function. Phosphorylation at Ser-601 by CK1 promotes coactivator function

Subunit：Present in a complex containing NCOA2, IKKA, IKKB, IKBKG and the histone acetyltransferase protein CREBBP (PubMed:11971985, PubMed:9346901).

Synonyms

AIB; AIB1; ANIB1

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/hct-116-cell.html [Content Brief]