Ninein Antibody (YA6689)

Cat. No.: HY-P86996: Data Sheet COA
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Ninein Antibody (YA6689) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ninein.

For research use only. We do not sell to patients.

Size	Price	Stock
20 μL		Ask For Quote & Lead Time
50 μL		Ask For Quote & Lead Time
100 μL		Ask For Quote & Lead Time

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Ninein Antibody (YA6689) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ninein.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 243 kDa

Species Reactivity

Human, Rat

SwissProt ID

Q8N4C6

Gene ID

51199 [NCBI]

Immunogen

Recombinant protein within human Ninein aa 1-500 / 2,090.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
FC FC: Flow Cytometry	1:1000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HeLa (lane 1(20μg)) and Rat brain (lane 2(20μg)) using Ninein Antibody (HY-P86996) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells (PubMed:15190203, PubMed:23386061). May also act as a centrosome maturation factor (PubMed:11956314). May play a role in microtubule nucleation, by recruiting the gamma-tubulin ring complex to the centrosome (PubMed:15190203). Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules (PubMed:15190203). Required for centriole organization and microtubule anchoring at the mother centriole (PubMed:23386061)

Subcellular Localization：Cytoplasm, cytoskeleton, microtubule organizing center, centrosome,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole,Cytoplasm

Expression：
Tissue_Specificity: Ubiquitous. Highly expressed in heart and skeletal muscle. Isoform 1 is more expressed than isoform 5

Isoforms & Post-Translational Modification：Q8N4C6 has 9 isomers: Q8N4C6-1: 243249 Da (predicted); Q8N4C6-2: 241191 Da (predicted); Q8N4C6-10: 246135 Da (predicted); Q8N4C6-4: 236135 Da (predicted); Q8N4C6-5: 243889 Da (predicted); Q8N4C6-6: 147799 Da (predicted); Q8N4C6-7: 248194 Da (predicted); Q8N4C6-9: 238941 Da (predicted); Q8N4C6-11: 160136 Da (predicted).
Phosphorylated by AURKA/Aurora kinase A and PKA kinases but not CK2 or AURKB/ Aurora kinase B

Subunit：Homooligomer

RRID

AB_3719243

Synonyms

Centrosome Marker antibody; Glycogen synthase kinase 3 beta interacting protein antibody; GSK3B interacting protein antibody; h Glycogen synthase kinase 3 beta-interacting protein antibody; hNinein antibody; KIAA1565 antibody; NIN antibody; NIN protein antibody; NIN_HUMAN antibody; ninein (GSK3B interacting protein) antibody; Centrosome Marker antibody; Glycogen synthase kinase 3 beta interacting protein antibody; GSK3B interacting protein antibody; h Glycogen synthase kinase 3 beta-interacting protein antibody; hNinein antibody; KIAA1565 antibody; NIN antibody; NIN protein antibody; NIN_HUMAN antibody; ninein (GSK3B interacting protein) antibody; Ninein antibody; Ninein centrosomal protein antibody; SCKL7 antibody;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)