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Met（c-Met） Antibody (YA3711)

(Synonyms: HGFR; AUTS9; RCCP2; c-Met; DFNB97)
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Based on 1 Customer Validation

Met（c-Met） Antibody (YA3711) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Met（c-Met）.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human, Monkey

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
IHC-P
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
  • Met（c-Met） Antibody (YA3711) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

Met（c-Met） Antibody (YA3711) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Met（c-Met）.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Monkey
  • Observed Molecular Weight
    Observed band size: 170/140 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 156 kDa
Immunogen

Purified recombinant fragment of human MET (AA: 743-932) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3718814

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using Met (c-Met) Antibody (HY-P84014, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Met (c-Met) Antibody (HY-P84014, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
    Immunohistochemical analysis of paraffin-embedded human glioma tissue using Met (c-Met) Antibody (HY-P84014, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using Met (c-Met) Antibody (HY-P84014, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue using Met (c-Met) Antibody (HY-P84014, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue using Met (c-Met) Antibody (HY-P84014, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    c-Met (hepatocyte growth factor receptor, HGFR) is a protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. c-Met is a membrane receptor that is essential for embryonic development and wound healing. Hepatocyte growth factor (HGF) is the only known ligand of the c-Met receptor. c-Met is normally expressed by cells of epithelial origin, while expression of HGF is restricted to cells of mesenchymalorigin. Upon HGF stimulation, c-Met induces several biological responses that collectively give rise to a program known as invasive growth.

  • Subcellular Localization

    Membrane; Single-pass type I membrane protein; Secreted

  • Expression


    Tissue_specificity:It is expressed in normal hepatocytes, as well as in the epithelial cells of the stomach, small intestine, and large intestine. It is also present in the basal keratinocytes of the esophagus and skin. Higher concentrations are found in the liver, gastrointestinal tract, thyroid gland, and kidneys. It is also expressed in brain tissue. It is also expressed in the metaphysis (protein level) (PubMed:26637977) .

  • Isoforms & Post-Translational Modification

    c-Met has 3 isomers: P08581-1: 155541 Da (predicted); P08581-2: 157712 Da (predicted); P08581-3: 85745 Da (predicted).
    Autophosphorylated in response to ligand binding on Tyr-1234 and Tyr-1235 in the kinase domain leading to further phosphorylation of Tyr-1349 and Tyr-1356 in the C-terminal multifunctional docking site. Dephosphorylated by PTPRJ at Tyr-1349 and Tyr-1365. Dephosphorylated by PTPN1 and PTPN2;Ubiquitinated. Ubiquitination by CBL regulates MET endocytosis, resulting in decreasing plasma membrane receptor abundance, and in endosomal degradation and/or recycling of internalized receptors;O-mannosylation of IPT/TIG domains by TMEM260 is required for protein maturation (PubMed:37186866). O-mannosylated residues are composed of single mannose glycans that are not elongated or modified (PubMed:37186866);(Microbial infection) Tyrosine phosphorylation is stimulated by L.monocytogenes InlB. Tyrosine phosphorylation is maximal 10-20 minutes after treatment with InlB and disappears by 60 minutes. The phosphorylated residues were not identified

  • Subunit

    Heterodimer made of an alpha chain (50 kDa) and a beta chain (145 kDa) which are disulfide linked. Binds PLXNB1. Interacts when phosphorylated with downstream effectors including STAT3, PIK3R1, SRC, PCLG1, GRB2 and GAB1.

  • SwissProt ID

    P08581

  • Gene ID
    4233 [NCBI]
  • Synonyms

    HGFR; AUTS9; RCCP2; c-Met; DFNB97

Met（c-Met） Antibody (YA3711) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using Met (c-Met) Antibody (HY-P84014, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Met (c-Met) Antibody (HY-P84014, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human glioma tissue using Met (c-Met) Antibody (HY-P84014, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using Met (c-Met) Antibody (HY-P84014, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human skin cancer tissue using Met (c-Met) Antibody (HY-P84014, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue using Met (c-Met) Antibody (HY-P84014, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Met（c-Met） Antibody (YA3711) Product Image
    1/16
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
  • Met（c-Met） Antibody (YA3711) Product Image
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using Met (c-Met) Antibody (HY-P84014, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Met (c-Met) Antibody (HY-P84014, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human glioma tissue using Met (c-Met) Antibody (HY-P84014, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using Met (c-Met) Antibody (HY-P84014, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human skin cancer tissue using Met (c-Met) Antibody (HY-P84014, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue using Met (c-Met) Antibody (HY-P84014, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
  • Experimental Validation Results for Met（c-Met） Antibody (YA3711)
  • Met（c-Met） Antibody (YA3711) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using Met (c-Met) Antibody (HY-P84014, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Met（c-Met） Antibody (YA3711) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Met (c-Met) Antibody (HY-P84014, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Met（c-Met） Antibody (YA3711) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human glioma tissue using Met (c-Met) Antibody (HY-P84014, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Met（c-Met） Antibody (YA3711) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using Met (c-Met) Antibody (HY-P84014, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Met（c-Met） Antibody (YA3711) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue using Met (c-Met) Antibody (HY-P84014, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Met（c-Met） Antibody (YA3711) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue using Met (c-Met) Antibody (HY-P84014, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Met（c-Met） Antibody (YA3711) Product Image IHC-P
  • Met（c-Met） Antibody (YA3711) Product Image IHC-P
  • Met（c-Met） Antibody (YA3711) Product Image IHC-P
  • Met（c-Met） Antibody (YA3711) Product Image IHC-P
  • Met（c-Met） Antibody (YA3711) Product Image IHC-P
  • Met（c-Met） Antibody (YA3711) Product Image IHC-P