SGLT2 Antibody
(Synonyms: SGLT2, SLC5A2, Sodium/glucose cotransporter 2, Na(+)/glucose cotransporter 2, Low affinity sodium-glucose cotransporter, Solute carrier family 5 member 2)SGLT2 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to SGLT2.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:50-200 |
Product Details
SGLT2 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to SGLT2.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 72; 49 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 72; 49 kDa
Synthetic peptide corresponding to the center region of human SGLT2.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
SGLT2 is an Electrogenic Na(+)-coupled sugar symporter that actively transports D-glucose at the plasma membrane, with a Na(+) to sugar coupling ratio of 1:1. Transporter activity is driven by a transmembrane Na(+) electrochemical gradient set by the Na(+)/K(+) pump. Unlike SLC5A1/SGLT1, requires the auxiliary protein PDZK1IP1/MAP17 for full transporter activity. Has a primary role in D-glucose reabsorption from glomerular filtrate across the brush border of the early proximal tubules of the kidney (By similarity)[1][2][3][4][5].
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Subcellular Localization
Apical cell membrane
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Isoforms & Post-Translational Modification
SGLT2 has 2 isoforms, P31639-1: amino acid length is 672, molecular weight is 72897 Da (predicted); P31639-2: amino acid length is 458, molecular weight is 49357 Da (predicted).SGLT2 存在 2 个异构体，P31639-1：氨基酸个数为 672 个，分子量为 72897 Da (预测)；P31639-2：氨基酸个数为 458 个，分子量为 49357 Da (预测)。
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Subunit
Forms a heterodimer (via TM13) with PDZK1IP1 (via N-terminal transmembrane helix); this interaction enhances SLC5A2 transporter activity
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SwissProt ID
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Synonyms
SGLT2, SLC5A2, Sodium/glucose cotransporter 2, Na(+)/glucose cotransporter 2, Low affinity sodium-glucose cotransporter, Solute carrier family 5 member 2
Documentation
[1]. Hummel CS, et al. Glucose transport by human renal Na+/D-glucose cotransporters SGLT1 and SGLT2. Am J Physiol Cell Physiol. 2011 Jan;300(1):C14-21. [Content Brief]
[2]. Coady MJ, et al. Characterization of the transport activity of SGLT2/MAP17, the renal low-affinity Na(+)-glucose cotransporter. Am J Physiol Renal Physiol. 2017 Aug 1;313(2):F467-F474. [Content Brief]
[3]. Niu Y, et al. Structural basis of inhibition of the human SGLT2-MAP17 glucose transporter. Nature. 2022 Jan;601(7892):280-284. [Content Brief]
[4]. Cui W, et al. Structures of human SGLT in the occluded state reveal conformational changes during sugar transport. Nat Commun. 2023 May 22;14(1):2920. [Content Brief]
[5]. Hiraizumi M, et al. Transport and inhibition mechanism of the human SGLT2-MAP17 glucose transporter. Nat Struct Mol Biol. 2024 Jan;31(1):159-169. [Content Brief]
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