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Glucose Transporter GLUT4 Antibody

(Synonyms: SLC2A4; GLUT4; Solute carrier family 2; facilitated glucose transporter member 4; Glucose transporter type 4; insulin-responsive; GLUT-4)
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Based on 1 Customer Validation

Glucose Transporter GLUT4 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Glucose Transporter GLUT4.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody
  • Glucose Transporter GLUT4 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:1000 1:50-1:100 1:10000

Product Details

Description

Glucose Transporter GLUT4 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Glucose Transporter GLUT4.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 55 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to the N-terminal region of Human SLC2A4.AA range:21-70.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102158

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody
    Western blot analysis of extracts from Mouse heart (lane 2, 20ug), Hela (lane 3, 20ug), SK-Br-3 (lane 4, 20ug) and HEK293 (lane 5, 20ug) using Glucose Transporter GLUT4 Antibody (HY-P80689). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Glucose Transporter GLUT4 plays a role of stimulator of transcription factors and nuclear receptors activities. Activates transcriptional activity of estrogen receptor alpha, nuclear respiratory factor 1 (NRF1) and glucocorticoid receptor in the presence of glucocorticoids. May play a role in constitutive non-adrenergic-mediated mitochondrial biogenesis as suggested by increased basal oxygen consumption and mitochondrial number when overexpressed. May be involved in fat oxidation and non-oxidative glucose metabolism and in the regulation of energy expenditure. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:It is widely distributed throughout the body, with higher expression in the heart, brain, and skeletal muscle.

    Induction:Repressed by saturated fatty acids such as palmitate and stearate in skeletal muscle cells. Induced by insulin and reduced by aging in skeletal muscle biopsies. Down-regulated in type 2 diabetes mellitus subjects as well as in pre-diabetics

  • Isoforms & Post-Translational Modification

    Q86YN6 has 6 isomers: Q86YN6-1: 113222 Da (predicted); Q86YN6-2: 110981 Da (predicted); Q86YN6-3: 112568 Da (predicted); Q86YN6-4: 110327 Da (predicted); Q86YN6-5: 108919 Da (predicted); Q86YN6-6: 106230 Da (predicted).

  • Subunit

    Interacts with hepatocyte nuclear factor 4-alpha/HNF4A, Sterol regulatory binding transcription factor 1/SREBF1, PPAR-alpha/PPARA, thyroid hormone receptor beta/THRB and host cell factor/HCFC1. Interacts with estrogen-related receptor gamma/ESRRG and alpha/ESRRA. Interacts with PRDM16 (By similarity). Interacts with estrogen receptor alpha/ESR1

  • SwissProt ID

    P14672

  • Gene ID
    6517 [NCBI]
  • Synonyms

    SLC2A4; GLUT4; Solute carrier family 2; facilitated glucose transporter member 4; Glucose transporter type 4; insulin-responsive; GLUT-4

  • Research Field

    Signal Transduction

Glucose Transporter GLUT4 Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Mouse heart (lane 2, 20ug), Hela (lane 3, 20ug), SK-Br-3 (lane 4, 20ug) and HEK293 (lane 5, 20ug) using Glucose Transporter GLUT4 Antibody (HY-P80689). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Glucose Transporter GLUT4 Antibody Product Image
    1/16
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody
  • Glucose Transporter GLUT4 Antibody Product Image
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Mouse heart (lane 2, 20ug), Hela (lane 3, 20ug), SK-Br-3 (lane 4, 20ug) and HEK293 (lane 5, 20ug) using Glucose Transporter GLUT4 Antibody (HY-P80689). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody
  • Glucose Transporter GLUT4 Antibody Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Mouse heart (lane 2, 20ug), Hela (lane 3, 20ug), SK-Br-3 (lane 4, 20ug) and HEK293 (lane 5, 20ug) using Glucose Transporter GLUT4 Antibody (HY-P80689). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Glucose Transporter GLUT4 Antibody Product Image WB