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IKK β Antibody (YA7485)

(Synonyms: IKKB, IKBKB, Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, I-kappa-B kinase 2, Nuclear factor NF-kappa-B inhibitor kinase beta, Serine/threonine protein kinase IKBKB, IKK-2, IKK2, NFKBIKB)

IKK β Antibody (YA7485) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to IKK β.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for IKK β Antibody (YA7485)
  • IKK β Antibody (YA7485) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:2000-1:10000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

IKK β Antibody (YA7485) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to IKK β.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 87 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 87 kDa
Immunogen

The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for IKK β Antibody (YA7485)
    Western blot analysis was performed on extracts from HL60 (lane 1, 20μg), HT22 (lane 2, 20μg), HCT116 (lane 3, 20μg), A594 (lane 4, 20μg), using IKK β Antibody (YA7485). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 25°C for 1hour.The primary antibody (1:2000 dilution) and the loading control antibody (GAPDH, HY-P83944, 1:3000 dilution) were incubated in 5% non-fat milk in TBST for overnight. AF800-conjugated Goat Anti-Rabbit IgG H&L(HY-P810687, 1:1000 dilution) and AF680-conjugated Goat Anti-Mouse IgG H&L(HY-P89186 , 1:10000 dilution) was then applied for 1 hour at 37°C, protect from light.

Background

  • Function

    IKK-β (IKBKB) is a catalytic subunit of the IκB kinase (IKK) complex and functions as a central signal integration node that regulates canonical NF-κB signaling, inflammatory responses, cell survival, and immune activation[1]. Mechanistically, activated IKK-β phosphorylates inhibitory IκB proteins, promoting their ubiquitin-dependent degradation and releasing NF-κB transcription factors for nuclear translocation and target gene expression[2]. Through this pathway, IKK-β links extracellular inflammatory stimuli to transcriptional programs that control cytokine production, stress responses, and cellular adaptation[1][3]. In disease contexts, dysregulated IKK-β/NF-κB signaling has been associated with chronic inflammation, cancer, metabolic disorders, cardiovascular disease, and pathogen-driven pathology, making the kinase a widely studied experimental target[1][3]. Compared with the related catalytic isoform IKK-α (CHUK), IKK-β is the predominant kinase mediating canonical NF-κB activation in a NEMO-dependent manner, whereas IKK family members also participate in additional signaling networks beyond classical NF-κB regulation[4]. This functional distinction is important for mechanistic studies aimed at dissecting pathway-specific signaling outputs and inflammatory phenotypes[4]. For experimental applications, pharmacological or genetic inhibition of IKK-β is commonly used to suppress NF-κB activation and evaluate the contribution of inflammatory signaling to disease models, while the expanding spectrum of IKK substrates continues to inform the interpretation of inhibitor-based studies[3][4].

  • Subcellular Localization

    Cytoplasm,Nucleus,Membrane raft

  • Expression


    Tissue_Specificity: Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood

  • Isoforms & Post-Translational Modification

    O14920 has four isomers: O14920-1: 86564 Da (predicted); O14920-2: 85945 Da (predicted); O14920-3: 29236 Da (predicted); O14920-4: 79509 Da (predicted).
    Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK as well as TBK1 and PRKCZ; which enhances activity (PubMed:10022904, PubMed:16207722)丨(Microbial infection) Acetylation of Thr-180 by Yersinia YopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway丨Ubiquitinated丨(Microbial infection) Monoubiquitination by TRIM21 is disrupted by Yersinia YopJ丨Hydroxylated by PHD1/EGLN2, loss of hydroxylation under hypoxic conditions results in activation of NF-kappa-B

  • Subunit

    Component of the I-kappa-B-kinase (IKK) core complex consisting of CHUK, IKBKB and IKBKG; probably four alpha/CHUK-beta/IKBKB dimers are associated with four gamma/IKBKG subunits (PubMed:32935379)

  • SwissProt ID

    O14920

  • Synonyms

    IKKB, IKBKB, Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, I-kappa-B kinase 2, Nuclear factor NF-kappa-B inhibitor kinase beta, Serine/threonine protein kinase IKBKB, IKK-2, IKK2, NFKBIKB

References

[1]. Zhang J, et al. IκB kinase β (IKKβ): Structure, transduction mechanism, biological function, and discovery of its inhibitors. Int J Biol Sci. 2023 Aug 6;19(13):4181-4203. [Content Brief]

[2]. IKK-β gene information from NCBI.

[3]. Hinz M. The IκB kinase complex in NF-κB regulation and beyond. EMBO Rep. 2014 Jan;15(1):46-61. [Content Brief]

[4]. Antonia RJ, et al. Expanding the View of IKK: New Substrates and New Biology. Trends Cell Biol. 2021 Mar;31(3):166-178. [Content Brief]

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IKK β Antibody (YA7485) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from HL60 (lane 1, 20μg), HT22 (lane 2, 20μg), HCT116 (lane 3, 20μg), A594 (lane 4, 20μg), using IKK β Antibody (YA7485). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 25°C for 1hour.The primary antibody (1:2000 dilution) and the loading control antibody (GAPDH, HY-P83944, 1:3000 dilution) were incubated in 5% non-fat milk in TBST for overnight. AF800-conjugated Goat Anti-Rabbit IgG H&L(HY-P810687, 1:1000 dilution) and AF680-conjugated Goat Anti-Mouse IgG H&L(HY-P89186 , 1:10000 dilution) was then applied for 1 hour at 37°C, protect from light.

  • IKK β Antibody (YA7485) Product Image
    1/16
  • Experimental Validation Results for IKK β Antibody (YA7485)
  • IKK β Antibody (YA7485) Product Image
  • Experimental Validation Results for IKK β Antibody (YA7485)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from HL60 (lane 1, 20μg), HT22 (lane 2, 20μg), HCT116 (lane 3, 20μg), A594 (lane 4, 20μg), using IKK β Antibody (YA7485). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 25°C for 1hour.The primary antibody (1:2000 dilution) and the loading control antibody (GAPDH, HY-P83944, 1:3000 dilution) were incubated in 5% non-fat milk in TBST for overnight. AF800-conjugated Goat Anti-Rabbit IgG H&L(HY-P810687, 1:1000 dilution) and AF680-conjugated Goat Anti-Mouse IgG H&L(HY-P89186 , 1:10000 dilution) was then applied for 1 hour at 37°C, protect from light.
  • Experimental Validation Results for IKK β Antibody (YA7485)
  • IKK β Antibody (YA7485) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from HL60 (lane 1, 20μg), HT22 (lane 2, 20μg), HCT116 (lane 3, 20μg), A594 (lane 4, 20μg), using IKK β Antibody (YA7485). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 25°C for 1hour.The primary antibody (1:2000 dilution) and the loading control antibody (GAPDH, HY-P83944, 1:3000 dilution) were incubated in 5% non-fat milk in TBST for overnight. AF800-conjugated Goat Anti-Rabbit IgG H&L(HY-P810687, 1:1000 dilution) and AF680-conjugated Goat Anti-Mouse IgG H&L(HY-P89186 , 1:10000 dilution) was then applied for 1 hour at 37°C, protect from light.

  • IKK β Antibody (YA7485) Product Image WB