PHF8 Antibody (YA2324)
(Synonyms: Histone lysine demethylase PHF8; MRXSSD; PHD finger protein 8; PHF8; ZNF422)
Based on 1 Customer Validation
PHF8 Antibody (YA2324) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PHF8.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, IP
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IP
IP: Immunoprecipitation
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|---|---|---|---|
| Dilution Ratio | 1:1000-1:2000 | 1:50-1:200 | 1:70 |
Product Details
PHF8 Antibody (YA2324) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PHF8.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 140 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 118 kDa
Entrez Gene: 23133 Human ; 320595 Mouse ; 74042 Mouse ; 317425 Rat
SwissProt: Q9UPP1 Human ; Q80TJ7 Mouse ;
OMIM: 300263 Human
A synthesized peptide derived from human PHF8 aa800-860.
Endogenous
Affinity Chromatography
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
PHF8 is a Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes. Required for brain development, probably by regulating expression of neuron-specific genes. Only has activity toward H4K20Me1 when nucleosome is used as a substrate and when not histone octamer is used as substrate. May also have weak activity toward dimethylated H3 'Lys-36' (H3K36Me2), however, the relevance of this result remains unsure in vivo. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3. Positively modulates transcription of histone demethylase KDM5C, acting synergistically with transcription factor ARX; synergy may be related to enrichment of histone H3K4me3 in regulatory elements
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Subcellular Localization
Nucleus; Nucleus, nucleolus
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Isoforms & Post-Translational Modification
Q9UPP1 has 5 isomers: Q9UPP1-1: 117864 Da (predicted); Q9UPP1-2: 113913 Da (predicted); Q9UPP1-3: 106902 Da (predicted); Q9UPP1-4: 105913 Da (predicted); Q9UPP1-5: 98290 Da (predicted).
Phosphorylation at Ser-69 and Ser-120 are required for dissociation from chromatin and accumulation of H4K20Me1 levels during prophase -
Subunit
Interacts with POLR1B, UBTF, SETD1A, HCFC1, E2F1 and ZNF711. Interacts with ZNF263; recruited to the SIX3 promoter along with other proteins involved in chromatin modification and transcriptional corepression where it contributes to transcriptional repression (PubMed:32051553)
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SwissProt ID
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Synonyms
Histone lysine demethylase PHF8; MRXSSD; PHD finger protein 8; PHF8; ZNF422
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Research Field
Epigenetics and Nuclear Signaling
Documentation
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