KLF4 Antibody (YA6358)(PBS only)

Cat. No.: HY-P86666A: Data Sheet User Guide for Antibodies
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KLF4 Antibody (YA6358) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KLF4.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

KLF4 Antibody (YA6358) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KLF4.

Host

Rabbit

Molecular Weight

Predicted band size: 56 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O43474

Gene ID

9314 [NCBI]

Immunogen

Recombinant protein within human KLF4 aa 1-500

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-200
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200
FC FC: Flow Cytometry	1ug:Test

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription

Subcellular Localization：Nucleus; Cytoplasm

Isoforms & Post-Translational Modification：O43474 has 5 isomers: O43474-3: 54671 Da (predicted); O43474-1: 51076 Da (predicted); O43474-4: 45639 Da (predicted); O43474-5: 12329 Da (predicted); O43474-6: 6489 Da (predicted).
Ubiquitinated. 'Lys-48'-linked ubiquitinated and targeted for proteasomal degradation by the SCF(BTRC) E3 ubiquitin-protein ligase complex, thereby negatively regulating cell pluripotency maintenance and embryogenesis;Polyglutamylated by TTLL1 and TTLL4 at Glu-411, which inhibits KLF4 binding with E3 ligase component BTRC, thereby impeding ubiquitination. Deglutamylated by CCP1 and CCP6; deglutamylation promotes KLF4 ubiquitination. KLF4 glutamylation state plays a critical role in the regulation of its function in cell reprogramming, pluripotency maintenance and embryogenesis

Subunit：Interacts with POU5F1/OCT4 and SOX2 (By similarity). Interacts with MUC1 (via the C-terminal domain) (PubMed:17308127). Interacts with MEIS2 isoform 4 and PBX1 isoform PBX1a (PubMed:21746878). Interacts with ZNF296 (By similarity). Interacts with GLIS1 (PubMed:21654807). Interacts with BTRC; this interaction leads to KLF4 ubiquitination and subsequent degradation (By similarity). Interacts with IPO7; the interaction facilitates nuclear translocation of KLF4 in dental papilla cells (By similarity)

Synonyms

Endothelial Kruppel like zinc finger protein; EZF; GKLF; KLF; Kruppel like factor 4(Epithelial zinc finger protein EZF)(Gut enriched Krueppel like factor); Kruppel like factor 4(gut); Krueppel-like factor 4; EZF; GKLF; KLF 4; KLF-4; Krueppel like factor 4; KLF4_HUMAN.

Documentation

User Guide for Antibodies (1077 KB)