EI2BG Antibody (YA4519)

Cat. No.: HY-P84822: Fiche technique User Guide for Antibodies
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EI2BG Antibody (YA4519) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to EI2BG.

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Size	Stock	Quantité
20 μL	Obtenir un devis 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Obtenir un devis 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Obtenir un devis 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Obtenir un devis

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Description
Références

Description

EI2BG Antibody (YA4519) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to EI2BG.

Host

Mouse

Clonality

Monoclonal

Masse moléculaire

Predicted band size: 50 kDa;

Observed band size: 48 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9NR50

Gene ID

8891 [NCBI]

Immunogen

Purified recombinant fragment of human EI2BG aa 110-452(KLH ).

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
FC FC: Flow Cytometry	1:200-1:400

Pureté	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Background

Function：Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on the eukaryotic initiation factor 2 (eIF2) complex gamma subunit (PubMed:25858979, PubMed:27023709, PubMed:31048492). Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed (PubMed:25858979, PubMed:31048492)

Subcellular Localization：Cytoplasm, cytosol

Isoforms & Post-Translational Modification：Q9NR50 has 3 isomers: Q9NR50-1: 50240 Da (predicted); Q9NR50-2: 46145 Da (predicted); Q9NR50-3: 44803 Da (predicted).

Subunit：Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex (PubMed:25858979, PubMed:27023709, PubMed:31048492). Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides (PubMed:31048492)

Synonyms

EIF2B3; EIF-2B; EIF2Bgamma

Documentation

User Guide for Antibodies (1077 KB)

Références

[1]. /biology-dictionary/hct-116-cell.html [Content Brief]