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Cyclin B1 Antibody

(Synonyms: CCNB1; CCNB; G2/mitotic-specific cyclin-B1)
Customer Review

Based on 1 Customer Validation

Cyclin B1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Cyclin B1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Cyclin B1 Antibody
  • Experimental Validation Results for Cyclin B1 Antibody
  • Experimental Validation Results for Cyclin B1 Antibody
  • Cyclin B1 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200 1:10000

Product Details

Description

Cyclin B1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Cyclin B1.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 60 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 48 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Cyclin B1.AA range:91-140

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102637

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cyclin B1 Antibody
    Western blot analysis of extracts from Ramos (lane 1) and Hela (lane 2) and Raji (lane 3) and Jurkat (lane 4) using Cyclin B1antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for Cyclin B1 Antibody
    Immunocytochemistry analysis of Hela cells labeling Cyclin B1 with Cyclin B1 Antibody (HY-P80632) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cyclin B1 Antibody (HY-P80632) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Cyclin B1 Antibody
    Immunocytochemistry analysis of HepG2 cells labeling Cyclin B1 with Cyclin B1 Antibody (HY-P80632) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cyclin B1 Antibody (HY-P80632) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Cyclin B1 is essential for the control of the cell cycle at the G2/M (mitosis) transition

  • Subcellular Localization

    Cytoplasm; Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

  • Isoforms & Post-Translational Modification

    P14635 has 2 isomers: P14635-1: 48337 Da (predicted); P14635-2: 44102 Da (predicted).
    Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction (PubMed:16009132). Deubiquitinated by USP22 during G2/M phase (PubMed:27030811);Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site

  • Subunit

    Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase. Interacts with CCNF; interaction is required for nuclear localization. Interacts with CDK5RAP3 (PubMed:15790566). Interacts with RFPL4A and UBE2A (By similarity). Interacts with INCA1 (PubMed:21540187)

  • SwissProt ID

    P14635

  • Gene ID
    891 [NCBI]
  • Synonyms

    CCNB1; CCNB; G2/mitotic-specific cyclin-B1

  • Research Field

    Cell Biology

Cyclin B1 Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Ramos (lane 1) and Hela (lane 2) and Raji (lane 3) and Jurkat (lane 4) using Cyclin B1antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Cyclin B1 with Cyclin B1 Antibody (HY-P80632) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cyclin B1 Antibody (HY-P80632) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Cyclin B1 with Cyclin B1 Antibody (HY-P80632) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cyclin B1 Antibody (HY-P80632) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Cyclin B1 Antibody Product Image
    1/16
  • Experimental Validation Results for Cyclin B1 Antibody
  • Experimental Validation Results for Cyclin B1 Antibody
  • Experimental Validation Results for Cyclin B1 Antibody
  • Cyclin B1 Antibody Product Image
  • Experimental Validation Results for Cyclin B1 Antibody
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Ramos (lane 1) and Hela (lane 2) and Raji (lane 3) and Jurkat (lane 4) using Cyclin B1antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for Cyclin B1 Antibody
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Cyclin B1 with Cyclin B1 Antibody (HY-P80632) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cyclin B1 Antibody (HY-P80632) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Cyclin B1 Antibody
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Cyclin B1 with Cyclin B1 Antibody (HY-P80632) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cyclin B1 Antibody (HY-P80632) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Cyclin B1 Antibody
  • Experimental Validation Results for Cyclin B1 Antibody
  • Experimental Validation Results for Cyclin B1 Antibody
  • Cyclin B1 Antibody Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Ramos (lane 1) and Hela (lane 2) and Raji (lane 3) and Jurkat (lane 4) using Cyclin B1antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • Cyclin B1 Antibody Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Cyclin B1 with Cyclin B1 Antibody (HY-P80632) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cyclin B1 Antibody (HY-P80632) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Cyclin B1 Antibody Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling Cyclin B1 with Cyclin B1 Antibody (HY-P80632) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cyclin B1 Antibody (HY-P80632) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Cyclin B1 Antibody Product Image WB
  • Cyclin B1 Antibody Product Image ICC
  • Cyclin B1 Antibody Product Image ICC