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MYSM1/ KIAA1915 Antibody

(Synonyms: MYSM1, KIAA1915, Histone H2A deubiquitinase MYSM1, 2A-DUB, Myb-like, SWIRM and MPN domain-containing protein 1)
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Based on 1 Customer Validation

MYSM1/ KIAA1915 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to MYSM1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IF-Tissue, ELISA

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MYSM1/ KIAA1915 Antibody
  • MYSM1/ KIAA1915 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
Dilution Ratio 1:500-1:2000 1:100-1:300 1:40000 1:50-200

Product Details

Description

MYSM1/ KIAA1915 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to MYSM1.

  • Host Rabbit
  • Clonality polyclonal
  • Species Reactivity
    Human, Mouse
Immunogen

Recomninant protein of human MYSM1.AA range:520-569.

Sensitivity

Endogenous

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MYSM1/ KIAA1915 Antibody
    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), MCF-7 (lane 3), A549 (lane 4), 293T (lane 5), Jurkat (lane 6), and K562 (lane 7) using MYSM1/ KIAA1915 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    MYSM1/ KIAA1915 is a Metalloprotease with deubiquitinase activity that plays important regulator roles in hematopoietic stem cell function, blood cell production and immune response. Participates in the normal programming of B-cell responses to antigen after the maturation process (By similarity). Within the cytoplasm, plays critical roles in the repression of innate immunity and autoimmunity. Removes 'Lys-63'-linked polyubiquitins from TRAF3 and TRAF6 complexes (By similarity). Attenuates NOD2-mediated inflammation and tissue injury by promoting 'Lys-63'-linked deubiquitination of RIPK2 component (By similarity). Suppresses the CGAS-STING1 signaling pathway by cleaving STING1 'Lys-63'-linked ubiquitin chains. In the nucleus, acts as a hematopoietic transcription regulator derepressing a range of genes essential for normal stem cell differentiation including EBF1 and PAX5 in B-cells, ID2 in NK-cell progenitor or FLT3 in dendritic cell precursors. Deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, leading to dissociation of histone H1 from the nucleosome[1][2][3][4][5].

  • Subcellular Localization

    Nucleus; Cytoplasm

  • Expression


    Induction: By DNA from viral infection and intracellular DNA.

  • Isoforms & Post-Translational Modification

    MYSM1/ KIAA1915 has 3 isoforms, Q5VVJ2-1: amino acid length is 828, molecular weight is 95032 Da (predicted); Q5VVJ2-2: amino acid length is 575, molecular weight is 66421 Da (predicted); Q5VVJ2-3: amino acid length is 234, molecular weight is 26716 Da (predicted).

  • Subunit

    Component of a large chromatin remodeling complex, at least composed of MYSM1, PCAF, RBM10 and KIF11/TRIP5.

  • SwissProt ID

    Q5VVJ2

  • Synonyms

    MYSM1, KIAA1915, Histone H2A deubiquitinase MYSM1, 2A-DUB, Myb-like, SWIRM and MPN domain-containing protein 1

References

[1]. Nandakumar V, et al. Epigenetic control of natural killer cell maturation by histone H2A deubiquitinase, MYSM1. Proc Natl Acad Sci U S A. 2013 Oct 8;110(41):E3927-36. [Content Brief]

[2]. Le Guen T, et al. An in vivo genetic reversion highlights the crucial role of Myb-Like, SWIRM, and MPN domains 1 (MYSM1) in human hematopoiesis and lymphocyte differentiation. J Allergy Clin Immunol. 2015 Dec;136(6):1619-1626.e5. [Content Brief]

[3]. Bahrami E, et al. Myb-like, SWIRM, and MPN domains 1 (MYSM1) deficiency: Genotoxic stress-associated bone marrow failure and developmental aberrations. J Allergy Clin Immunol. 2017 Oct;140(4):1112-1119. [Content Brief]

[4]. Tian M, et al. MYSM1 Represses Innate Immunity and Autoimmunity through Suppressing the cGAS-STING Pathway. Cell Rep. 2020 Oct 20;33(3):108297. [Content Brief]

[5]. Zhu P, et al. A histone H2A deubiquitinase complex coordinating histone acetylation and H1 dissociation in transcriptional regulation. Mol Cell. 2007 Aug 17;27(4):609-21. [Content Brief]

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MYSM1/ KIAA1915 Antibody Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), MCF-7 (lane 3), A549 (lane 4), 293T (lane 5), Jurkat (lane 6), and K562 (lane 7) using MYSM1/ KIAA1915 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • MYSM1/ KIAA1915 Antibody Product Image
    1/16
  • Experimental Validation Results for MYSM1/ KIAA1915 Antibody
  • MYSM1/ KIAA1915 Antibody Product Image
  • Experimental Validation Results for MYSM1/ KIAA1915 Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), MCF-7 (lane 3), A549 (lane 4), 293T (lane 5), Jurkat (lane 6), and K562 (lane 7) using MYSM1/ KIAA1915 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for MYSM1/ KIAA1915 Antibody
  • MYSM1/ KIAA1915 Antibody Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), MCF-7 (lane 3), A549 (lane 4), 293T (lane 5), Jurkat (lane 6), and K562 (lane 7) using MYSM1/ KIAA1915 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • MYSM1/ KIAA1915 Antibody Product Image WB