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Carbonic Anhydrase 1 Antibody (YA1982)

(Synonyms: CA1; Carbonic anhydrase 1; Carbonate dehydratase I; Carbonic anhydrase B; CAB; Carbonic anhydrase I; CA-I)
Customer Review

Based on 1 Customer Validation

Carbonic Anhydrase 1 Antibody (YA1982) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Carbonic Anhydrase 1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat, Zebrafish

  • Formulation:

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
IHC-P
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
  • Carbonic Anhydrase 1 Antibody (YA1982) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000-1:2000 1:100-1:200 1:50-1:200

Product Details

Description

Carbonic Anhydrase 1 Antibody (YA1982) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Carbonic Anhydrase 1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Zebrafish
  • Observed Molecular Weight
    Observed band size: 29 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 29 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Carbonic Anhydrase 1 aa200-261/261.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
    Immunohistochemical analysis of paraffin-embedded mouse lung tissue using Carbonic Anhydrase 1 Antibody (HY-P82237, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
    Immunohistochemical analysis of paraffin-embedded human stomach tissue using Carbonic Anhydrase 1 Antibody (HY-P82237, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
    Immunohistochemical analysis of paraffin-embedded rat lung tissue using Carbonic Anhydrase 1 Antibody (HY-P82237, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
    Immunohistochemical analysis of paraffin-embedded rat rectum tissue using Carbonic Anhydrase 1 Antibody (HY-P82237, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
    Immunohistochemical analysis of paraffin-embedded rat colon tissue using Carbonic Anhydrase 1 Antibody (HY-P82237, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Carbonic Anhydrase 1 Antibody (HY-P82237, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Carbonic Anhydrase 1 catalyzes the reversible hydration of carbon dioxide. Can hydrate cyanamide to urea[1][2][3][4][5][6][7][8][9][10].

  • Subcellular Localization

    Cytoplasm

  • SwissProt ID

    P00915

  • Gene ID
    759 [NCBI]
  • Synonyms

    CA1; Carbonic anhydrase 1; Carbonate dehydratase I; Carbonic anhydrase B; CAB; Carbonic anhydrase I; CA-I

  • Research Field

    Cardiovascular

Carbonic Anhydrase 1 Antibody (YA1982) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse lung tissue using Carbonic Anhydrase 1 Antibody (HY-P82237, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human stomach tissue using Carbonic Anhydrase 1 Antibody (HY-P82237, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat lung tissue using Carbonic Anhydrase 1 Antibody (HY-P82237, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat rectum tissue using Carbonic Anhydrase 1 Antibody (HY-P82237, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat colon tissue using Carbonic Anhydrase 1 Antibody (HY-P82237, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Carbonic Anhydrase 1 Antibody (HY-P82237, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Carbonic Anhydrase 1 Antibody (YA1982) Product Image
    1/16
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
  • Carbonic Anhydrase 1 Antibody (YA1982) Product Image
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse lung tissue using Carbonic Anhydrase 1 Antibody (HY-P82237, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human stomach tissue using Carbonic Anhydrase 1 Antibody (HY-P82237, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat lung tissue using Carbonic Anhydrase 1 Antibody (HY-P82237, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat rectum tissue using Carbonic Anhydrase 1 Antibody (HY-P82237, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat colon tissue using Carbonic Anhydrase 1 Antibody (HY-P82237, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Carbonic Anhydrase 1 Antibody (HY-P82237, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
  • Experimental Validation Results for Carbonic Anhydrase 1 Antibody (YA1982)
  • Carbonic Anhydrase 1 Antibody (YA1982) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue using Carbonic Anhydrase 1 Antibody (HY-P82237, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Carbonic Anhydrase 1 Antibody (YA1982) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human stomach tissue using Carbonic Anhydrase 1 Antibody (HY-P82237, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Carbonic Anhydrase 1 Antibody (YA1982) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat lung tissue using Carbonic Anhydrase 1 Antibody (HY-P82237, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Carbonic Anhydrase 1 Antibody (YA1982) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat rectum tissue using Carbonic Anhydrase 1 Antibody (HY-P82237, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Carbonic Anhydrase 1 Antibody (YA1982) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat colon tissue using Carbonic Anhydrase 1 Antibody (HY-P82237, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Carbonic Anhydrase 1 Antibody (YA1982) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Carbonic Anhydrase 1 Antibody (HY-P82237, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Carbonic Anhydrase 1 Antibody (YA1982) Product Image IHC-P
  • Carbonic Anhydrase 1 Antibody (YA1982) Product Image IHC-P
  • Carbonic Anhydrase 1 Antibody (YA1982) Product Image IHC-P
  • Carbonic Anhydrase 1 Antibody (YA1982) Product Image IHC-P
  • Carbonic Anhydrase 1 Antibody (YA1982) Product Image IHC-P
  • Carbonic Anhydrase 1 Antibody (YA1982) Product Image IHC-P