Copy Link Print Information

Phospho-Paxillin (Tyr118) Antibody (YA3083)

(Synonyms: PXN; Paxillin)
Customer Review

Based on 1 Customer Validation

Phospho-Paxillin (Tyr118) Antibody (YA3083) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Paxillin (Tyr118).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Phospho-Paxillin (Tyr118) Antibody (YA3083)
  • Phospho-Paxillin (Tyr118) Antibody (YA3083) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Phospho-Paxillin (Tyr118) Antibody (YA3083) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Paxillin (Tyr118).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 68 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 65 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Paxillin HV-pY-SF.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

RRID

AB_3105451

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-Paxillin (Tyr118) Antibody (YA3083)
    Western blot analysis of extracts from C6 (lane 2(20μg)

Background

  • Function

    Paxillin is a Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Recruits other proteins such as TRIM15 to focal adhesion

  • Subcellular Localization

    Cytoplasm, cytoskeleton; Cell junction, focal adhesion; Cytoplasm, cell cortex

  • Isoforms & Post-Translational Modification

    P49023 has 4 isomers: P49023-1: 64505 Da (predicted); P49023-2: 60967 Da (predicted); P49023-3: 66212 Da (predicted); P49023-4: 46604 Da (predicted).
    Phosphorylated by MAPK1/ERK2 (By similarity). Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. Phosphorylation at Ser-250 by SLK is required for PXN redistribution and cell motility (PubMed:23128389). Phosphorylation at Ser-272 promotes focal adhesion disassembly during cell migration (PubMed:26598620)

  • Subunit

    Interacts in vitro with VCL/vinculin as well as to the SH3 domain of SRC and, when tyrosine phosphorylated, to the SH2 domain of CRK (PubMed:7534286). Interacts with GIT1 (PubMed:10938112). Interacts with NUDT16L1/SDOS (By similarity). Interacts with PTK2/FAK1 (PubMed:14527389). Interacts with PTK2B/PYK2 (PubMed:19358827). Interacts with ASAP2 (PubMed:10749932). Interacts with unphosphorylated ITGA4 (PubMed:10604475). Interacts with RNF5 (PubMed:12861019). Interacts with PDCD10 (PubMed:20489202). Interacts with NEK3, the interaction is prolactin-dependent (PubMed:17297458). Interacts with PTK6 (PubMed:15572663). Interacts with TGFB1I1 (By similarity). Interacts with SORBS1 (PubMed:17462669). Interacts with PARVB (PubMed:22869380). Interacts (via LD motif 4) with PARVA/PARVIN (PubMed:15817463, PubMed:18508764, PubMed:18940607). Interacts (via LD motif 4) with ILK (PubMed:15817463, PubMed:18508764, PubMed:18940607). Interacts (via cytoplasmic domain) with CEACAM1; the interaction is phosphotyrosyl-dependent (PubMed:11035932). Interacts with LIMA1; this complex stabilizes actin dynamics (PubMed:24694988). Interacts with CD36 (via C-terminus) (PubMed:20037584). Interacts with TRIM15 (PubMed:25015296). Interacts with PAK4; PAK4 acts as a scaffold to suppport PAXI phosphorylation at Ser-272 (PubMed:26598620)

  • SwissProt ID

    P49023

  • Gene ID
    5829 [NCBI]
  • Synonyms

    PXN; Paxillin

  • Research Field

    Signal Transduction

Phospho-Paxillin (Tyr118) Antibody (YA3083) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from C6 (lane 2(20μg)

  • Phospho-Paxillin (Tyr118) Antibody (YA3083) Product Image
    1/16
  • Experimental Validation Results for Phospho-Paxillin (Tyr118) Antibody (YA3083)
  • Phospho-Paxillin (Tyr118) Antibody (YA3083) Product Image
  • Experimental Validation Results for Phospho-Paxillin (Tyr118) Antibody (YA3083)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from C6 (lane 2(20μg)
  • Experimental Validation Results for Phospho-Paxillin (Tyr118) Antibody (YA3083)
  • Phospho-Paxillin (Tyr118) Antibody (YA3083) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from C6 (lane 2(20μg)

  • Phospho-Paxillin (Tyr118) Antibody (YA3083) Product Image WB