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  5. Phospho-Paxillin (Tyr118) Antibody (YA3083)

Phospho-Paxillin (Tyr118) Antibody (YA3083)

Cat. No.: HY-P83338
COA
SDS
User Guide for Antibodies Technical Support

Phospho-Paxillin (Tyr118) Antibody (YA3083) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Paxillin (Tyr118).

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

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  • Descripciòn

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Descripciòn

Phospho-Paxillin (Tyr118) Antibody (YA3083) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Paxillin (Tyr118).
Host

Rabbit
Clonality

Recombinant,Monoclonal
Peso molecular
Predicted band size: 65 kDa;
Observed band size: 68 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human Paxillin HV-pY-SF.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
Sensitivity Endogenous Pureza Affinity Chromatography
Conjugation Non-conjugated Modification Phosphorylated
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Envío

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from C6 (lane 2(20μg)

Background
Function：Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Recruits other proteins such as TRIM15 to focal adhesion
Subcellular Localization：Cytoplasm, cytoskeleton; Cell junction, focal adhesion; Cytoplasm, cell cortex
Isoforms & Post-Translational Modification：P49023 has 4 isomers: P49023-1: 64505 Da (predicted); P49023-2: 60967 Da (predicted); P49023-3: 66212 Da (predicted); P49023-4: 46604 Da (predicted).
Phosphorylated by MAPK1/ERK2 (By similarity). Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. Phosphorylation at Ser-250 by SLK is required for PXN redistribution and cell motility (PubMed:23128389). Phosphorylation at Ser-272 promotes focal adhesion disassembly during cell migration (PubMed:26598620)
Subunit：Interacts in vitro with VCL/vinculin as well as to the SH3 domain of SRC and, when tyrosine phosphorylated, to the SH2 domain of CRK (PubMed:7534286). Interacts with GIT1 (PubMed:10938112). Interacts with NUDT16L1/SDOS (By similarity). Interacts with PTK2/FAK1 (PubMed:14527389). Interacts with PTK2B/PYK2 (PubMed:19358827). Interacts with ASAP2 (PubMed:10749932). Interacts with unphosphorylated ITGA4 (PubMed:10604475). Interacts with RNF5 (PubMed:12861019). Interacts with PDCD10 (PubMed:20489202). Interacts with NEK3, the interaction is prolactin-dependent (PubMed:17297458). Interacts with PTK6 (PubMed:15572663). Interacts with TGFB1I1 (By similarity). Interacts with SORBS1 (PubMed:17462669). Interacts with PARVB (PubMed:22869380). Interacts (via LD motif 4) with PARVA/PARVIN (PubMed:15817463, PubMed:18508764, PubMed:18940607). Interacts (via LD motif 4) with ILK (PubMed:15817463, PubMed:18508764, PubMed:18940607). Interacts (via cytoplasmic domain) with CEACAM1; the interaction is phosphotyrosyl-dependent (PubMed:11035932). Interacts with LIMA1; this complex stabilizes actin dynamics (PubMed:24694988). Interacts with CD36 (via C-terminus) (PubMed:20037584). Interacts with TRIM15 (PubMed:25015296). Interacts with PAK4; PAK4 acts as a scaffold to suppport PAXI phosphorylation at Ser-272 (PubMed:26598620)
RRID
Database

Entrez Gene: 5829 Human ; 19303 Mouse ; 360820 Rat

SwissProt: P49023 Human ; Q8VI36 Mouse ; Q66H76 Rat

OMIM: 602505 Human

Research Field

Signal Transduction
Synonyms
PXN; Paxillin
Documentación
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)
Referencias

Phospho-Paxillin (Tyr118) Antibody (YA3083) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Nombre del producto:
Phospho-Paxillin (Tyr118) Antibody (YA3083)
Cat. No.:
HY-P83338
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