Copy Link Print Information

Paxillin Antibody (YA6161)

(Synonyms: PXN; Paxillin)
1 Cited Publications
Customer Review

Based on 1 publication(s) in Google Scholar

Paxillin Antibody (YA6161) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Paxillin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for Paxillin Antibody (YA6161)
  • Experimental Validation Results for Paxillin Antibody (YA6161)
  • Experimental Validation Results for Paxillin Antibody (YA6161)
  • Experimental Validation Results for Paxillin Antibody (YA6161)
  • Experimental Validation Results for Paxillin Antibody (YA6161)
  • Experimental Validation Results for Paxillin Antibody (YA6161)
  • Experimental Validation Results for Paxillin Antibody (YA6161)
  • Experimental Validation Results for Paxillin Antibody (YA6161)
  • Experimental Validation Results for Paxillin Antibody (YA6161)
  • Paxillin Antibody (YA6161) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) Paxillin Antibody (YA6161)

More

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:4000 1:1000-1:5000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

Paxillin Antibody (YA6161) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Paxillin.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 68 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 68 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719167

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Paxillin Antibody (YA6161)
    Western blot analysis of extracts from Hela (lane2(20μg), C6 (lane3(20μg), RAW264.7 (lane4(20μg) and A431 (lane5(20μg) using Paxillin Antibody (HY-P86469). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/3000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Paxillin Antibody (YA6161)
    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Paxillin Antibody (YA6161). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86469, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Paxillin Antibody (YA6161)
    Immunohistochemical analysis of paraffin-embedded rat brain tissue using Paxillin Antibody (YA6161). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86469, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Paxillin Antibody (YA6161)
    Immunohistochemical analysis of paraffin-embedded rat kidney tissue using Paxillin Antibody (YA6161). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86469, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Paxillin Antibody (YA6161)
    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Paxillin Antibody (YA6161). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86469, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Paxillin Antibody (YA6161)
    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue using Paxillin Antibody (YA6161). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86469, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Paxillin Antibody (YA6161)
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using Paxillin Antibody (YA6161). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86469, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Paxillin Antibody (YA6161)
    Immunocytochemistry analysis of C6 cells labeling Paxillin with Paxillin Antibody (HY-P86469) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Paxillin Antibody (HY-P86469) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Paxillin Antibody (YA6161)
    Immunocytochemistry analysis of C6 cells labeling Paxillin with Paxillin Antibody (HY-P86469) at 1/300 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Paxillin Antibody (HY-P86469) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Paxillin is a Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Recruits other proteins such as TRIM15 to focal adhesion

  • Subcellular Localization

    Cytoplasm, cytoskeleton; Cell junction, focal adhesion; Cytoplasm, cell cortex

  • Isoforms & Post-Translational Modification

    P49023 has 4 isomers: P49023-1: 64505 Da (predicted); P49023-2: 60967 Da (predicted); P49023-3: 66212 Da (predicted); P49023-4: 46604 Da (predicted).
    Phosphorylated by MAPK1/ERK2 (By similarity). Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. Phosphorylation at Ser-250 by SLK is required for PXN redistribution and cell motility (PubMed:23128389). Phosphorylation at Ser-272 promotes focal adhesion disassembly during cell migration (PubMed:26598620)

  • Subunit

    Interacts in vitro with VCL/vinculin as well as to the SH3 domain of SRC and, when tyrosine phosphorylated, to the SH2 domain of CRK (PubMed:7534286). Interacts with GIT1 (PubMed:10938112). Interacts with NUDT16L1/SDOS (By similarity). Interacts with PTK2/FAK1 (PubMed:14527389). Interacts with PTK2B/PYK2 (PubMed:19358827). Interacts with ASAP2 (PubMed:10749932). Interacts with unphosphorylated ITGA4 (PubMed:10604475). Interacts with RNF5 (PubMed:12861019). Interacts with PDCD10 (PubMed:20489202). Interacts with NEK3, the interaction is prolactin-dependent (PubMed:17297458). Interacts with PTK6 (PubMed:15572663). Interacts with TGFB1I1 (By similarity). Interacts with SORBS1 (PubMed:17462669). Interacts with PARVB (PubMed:22869380). Interacts (via LD motif 4) with PARVA/PARVIN (PubMed:15817463, PubMed:18508764, PubMed:18940607). Interacts (via LD motif 4) with ILK (PubMed:15817463, PubMed:18508764, PubMed:18940607). Interacts (via cytoplasmic domain) with CEACAM1; the interaction is phosphotyrosyl-dependent (PubMed:11035932). Interacts with LIMA1; this complex stabilizes actin dynamics (PubMed:24694988). Interacts with CD36 (via C-terminus) (PubMed:20037584). Interacts with TRIM15 (PubMed:25015296). Interacts with PAK4; PAK4 acts as a scaffold to suppport PAXI phosphorylation at Ser-272 (PubMed:26598620)

  • SwissProt ID

    P49023

  • Gene ID
    5829 [NCBI]
  • Synonyms

    PXN; Paxillin

Paxillin Antibody (YA6161) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2(20μg), C6 (lane3(20μg), RAW264.7 (lane4(20μg) and A431 (lane5(20μg) using Paxillin Antibody (HY-P86469). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/3000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Paxillin Antibody (YA6161). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86469, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using Paxillin Antibody (YA6161). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86469, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat kidney tissue using Paxillin Antibody (YA6161). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86469, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Paxillin Antibody (YA6161). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86469, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue using Paxillin Antibody (YA6161). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86469, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using Paxillin Antibody (YA6161). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86469, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of C6 cells labeling Paxillin with Paxillin Antibody (HY-P86469) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Paxillin Antibody (HY-P86469) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of C6 cells labeling Paxillin with Paxillin Antibody (HY-P86469) at 1/300 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Paxillin Antibody (HY-P86469) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Paxillin Antibody (YA6161) Product Image
    1/16
  • Experimental Validation Results for Paxillin Antibody (YA6161)
  • Experimental Validation Results for Paxillin Antibody (YA6161)
  • Experimental Validation Results for Paxillin Antibody (YA6161)
  • Experimental Validation Results for Paxillin Antibody (YA6161)
  • Experimental Validation Results for Paxillin Antibody (YA6161)
  • Experimental Validation Results for Paxillin Antibody (YA6161)
  • Experimental Validation Results for Paxillin Antibody (YA6161)
  • Experimental Validation Results for Paxillin Antibody (YA6161)
  • Experimental Validation Results for Paxillin Antibody (YA6161)
  • Paxillin Antibody (YA6161) Product Image
  • Experimental Validation Results for Paxillin Antibody (YA6161)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2(20μg), C6 (lane3(20μg), RAW264.7 (lane4(20μg) and A431 (lane5(20μg) using Paxillin Antibody (HY-P86469). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/3000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Paxillin Antibody (YA6161)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Paxillin Antibody (YA6161). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86469, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Paxillin Antibody (YA6161)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using Paxillin Antibody (YA6161). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86469, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Paxillin Antibody (YA6161)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat kidney tissue using Paxillin Antibody (YA6161). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86469, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Paxillin Antibody (YA6161)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Paxillin Antibody (YA6161). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86469, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Paxillin Antibody (YA6161)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue using Paxillin Antibody (YA6161). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86469, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Paxillin Antibody (YA6161)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using Paxillin Antibody (YA6161). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86469, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Paxillin Antibody (YA6161)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of C6 cells labeling Paxillin with Paxillin Antibody (HY-P86469) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Paxillin Antibody (HY-P86469) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Paxillin Antibody (YA6161)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of C6 cells labeling Paxillin with Paxillin Antibody (HY-P86469) at 1/300 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Paxillin Antibody (HY-P86469) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Paxillin Antibody (YA6161)
  • Experimental Validation Results for Paxillin Antibody (YA6161)
  • Experimental Validation Results for Paxillin Antibody (YA6161)
  • Experimental Validation Results for Paxillin Antibody (YA6161)
  • Experimental Validation Results for Paxillin Antibody (YA6161)
  • Experimental Validation Results for Paxillin Antibody (YA6161)
  • Experimental Validation Results for Paxillin Antibody (YA6161)
  • Experimental Validation Results for Paxillin Antibody (YA6161)
  • Experimental Validation Results for Paxillin Antibody (YA6161)
  • Paxillin Antibody (YA6161) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane2(20μg), C6 (lane3(20μg), RAW264.7 (lane4(20μg) and A431 (lane5(20μg) using Paxillin Antibody (HY-P86469). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/3000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • Paxillin Antibody (YA6161) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Paxillin Antibody (YA6161). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86469, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Paxillin Antibody (YA6161) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using Paxillin Antibody (YA6161). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86469, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Paxillin Antibody (YA6161) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue using Paxillin Antibody (YA6161). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86469, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Paxillin Antibody (YA6161) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Paxillin Antibody (YA6161). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86469, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Paxillin Antibody (YA6161) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue using Paxillin Antibody (YA6161). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86469, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Paxillin Antibody (YA6161) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using Paxillin Antibody (YA6161). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86469, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Paxillin Antibody (YA6161) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of C6 cells labeling Paxillin with Paxillin Antibody (HY-P86469) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Paxillin Antibody (HY-P86469) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Paxillin Antibody (YA6161) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of C6 cells labeling Paxillin with Paxillin Antibody (HY-P86469) at 1/300 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Paxillin Antibody (HY-P86469) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Paxillin Antibody (YA6161) Product Image WB
  • Paxillin Antibody (YA6161) Product Image IHC-P
  • Paxillin Antibody (YA6161) Product Image IHC-P
  • Paxillin Antibody (YA6161) Product Image IHC-P
  • Paxillin Antibody (YA6161) Product Image IHC-P
  • Paxillin Antibody (YA6161) Product Image IHC-P
  • Paxillin Antibody (YA6161) Product Image IHC-P
  • Paxillin Antibody (YA6161) Product Image ICC
  • Paxillin Antibody (YA6161) Product Image ICC