SPAK Antibody (YA2827)
(Synonyms: STK39; SPAK; STE20/SPS1-related proline-alanine-rich protein kinase; Ste-20-related kinase; DCHT; Serine/threonine-protein kinase 39)
Based on 1 Customer Validation
SPAK Antibody (YA2827) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SPAK.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, FC
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Reactivity :
Human
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Formulation:
Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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|---|---|---|---|
| Dilution Ratio | 1:1000-1:2000 | 1:100-1:200 | 1:50-1:100 |
Product Details
SPAK Antibody (YA2827) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SPAK.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 68 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 59 kDa
A synthesized peptide derived from human STK39 aa401-454.
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
SPAK is an Effector serine/threonine-protein kinase component of the WNK-SPAK/OSR1 kinase cascade, which is involved in various processes, such as ion transport, response to hypertonic stress and blood pressure. Specifically recognizes and binds proteins with a RFXV motif. Acts downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4): following activation by WNK kinases, catalyzes phosphorylation of ion cotransporters, such as SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A5/KCC2 or SLC12A6/KCC3, regulating their activity. Mediates regulatory volume increase in response to hyperosmotic stress by catalyzing phosphorylation of ion cotransporters SLC12A1/NKCC2, SLC12A2/NKCC1 and SLC12A6/KCC3 downstream of WNK1 and WNK3 kinases. Phosphorylation of Na-K-Cl cotransporters SLC12A2/NKCC1 and SLC12A2/NKCC1 promote their activation and ion influx; simultaneously, phosphorylation of K-Cl cotransporters SLC12A5/KCC2 and SLC12A6/KCC3 inhibit their activity, blocking ion efflux. Acts as a regulator of NaCl reabsorption in the distal nephron by mediating phosphorylation and activation of the thiazide-sensitive Na-Cl cotransporter SLC12A3/NCC in distal convoluted tubule cells of kidney downstream of WNK4. Mediates the inhibition of SLC4A4, SLC26A6 as well as CFTR activities. Phosphorylates RELT[1][2][3][4][5][6].
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Subcellular Localization
Cytoplasm; Nucleus
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Expression
Tissue_specificity:Predominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis -
Isoforms & Post-Translational Modification
Q9UEW8 has 2 isomers: Q9UEW8-1: 59474 Da (predicted); Q9UEW8-2: 57937 Da (predicted).
Phosphorylation at Thr-231 by WNK kinases (WNK1, WNK2, WNK3 or WNK4) is required for activation (PubMed:16832045, PubMed:17190791, PubMed:21321328, PubMed:34289367). Autophosphorylation at Thr-231 positively regulates its activity (By similarity). Phosphorylated at Ser-309 by PRKCQ (PubMed:14988727) -
Subunit
The phosphorylated form forms a complex with WNK2 (By similarity). Interacts with SORL1 (via cytosolic C-terminus) (PubMed:20385770)
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SwissProt ID
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Synonyms
STK39; SPAK; STE20/SPS1-related proline-alanine-rich protein kinase; Ste-20-related kinase; DCHT; Serine/threonine-protein kinase 39
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Research Field
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Documentation
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