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NrCAM Antibody (YA2444)

(Synonyms: Bravo; hBravo; Neuronal cell adhesion molecule; Ng CAM related; NgCAM related cell adhesion molecule; Nr CAM; Nrcam)
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Based on 1 Customer Validation

NrCAM Antibody (YA2444) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NrCAM.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for NrCAM Antibody (YA2444)
  • NrCAM Antibody (YA2444) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

NrCAM Antibody (YA2444) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NrCAM.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 144 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 144 kDa
Species Reactivity Database

Entrez Gene: 4897 Human

SwissProt: Q92823 Human

Immunogen

A synthesized peptide derived from human NRCAM aa800-880/1304.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104799

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NrCAM Antibody (YA2444)
    Western blot analysis of extracts from Rat brain (lane 2(20μg)) using NrCAM Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    NrCAM cell adhesion protein that is required for normal responses to cell-cell contacts in brain and in the peripheral nervous system. Plays a role in neurite outgrowth in response to contactin binding. Plays a role in mediating cell-cell contacts between Schwann cells and axons. Plays a role in the formation and maintenance of the nodes of Ranvier on myelinated axons. Nodes of Ranvier contain clustered sodium channels that are crucial for the saltatory propagation of action potentials along myelinated axons. During development, nodes of Ranvier are formed by the fusion of two heminodes. Required for normal clustering of sodium channels at heminodes; not required for the formation of mature nodes with normal sodium channel clusters. Required, together with GLDN, for maintaining NFASC and sodium channel clusters at mature nodes of Ranvier

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Cell projection, axon; Secreted

  • Expression


    Tissue_specificity:The substance was detected in all examined tissues. In the brain, it was detected in the amygdala, caudate nucleus, corpus callosum, hippocampus, hypothalamus, substantia nigra, subthalamic nucleus, and thalamus.

  • Isoforms & Post-Translational Modification

    Q92823 has 6 isomers: Q92823-1: 143890 Da (predicted); Q92823-2: 136614 Da (predicted); Q92823-3: 130668 Da (predicted); Q92823-4: 131046 Da (predicted); Q92823-5: 144375 Da (predicted); Q92823-6: 131744 Da (predicted).

  • Subunit

    Constituent of a NFASC/NRCAM/ankyrin-G complex. Detected in a complex with CNTN1 and PTPRB. Interacts with GLDN/gliomedin (By similarity). Interacts with MYOC (PubMed:23897819)

  • SwissProt ID

    Q92823

  • Gene ID
    4897 [NCBI]
  • Synonyms

    Bravo; hBravo; Neuronal cell adhesion molecule; Ng CAM related; NgCAM related cell adhesion molecule; Nr CAM; Nrcam

  • Research Field

    Neuroscience

NrCAM Antibody (YA2444) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Rat brain (lane 2(20μg)) using NrCAM Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • NrCAM Antibody (YA2444) Product Image
    1/16
  • Experimental Validation Results for NrCAM Antibody (YA2444)
  • NrCAM Antibody (YA2444) Product Image
  • Experimental Validation Results for NrCAM Antibody (YA2444)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Rat brain (lane 2(20μg)) using NrCAM Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for NrCAM Antibody (YA2444)
  • NrCAM Antibody (YA2444) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Rat brain (lane 2(20μg)) using NrCAM Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • NrCAM Antibody (YA2444) Product Image WB