NrCAM Antibody (YA2444)

Cat. No.: HY-P82699: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

NrCAM Antibody (YA2444) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NrCAM.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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NrCAM Antibody (YA2444) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

NrCAM Antibody (YA2444) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NrCAM.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 144 kDa;

Observed band size: 144 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q92823

Gene ID

4897 [NCBI]

Immunogen

A synthesized peptide derived from human NRCAM aa800-880/1304.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Rat brain (lane 2(20μg)) using NrCAM Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Cell adhesion protein that is required for normal responses to cell-cell contacts in brain and in the peripheral nervous system. Plays a role in neurite outgrowth in response to contactin binding. Plays a role in mediating cell-cell contacts between Schwann cells and axons. Plays a role in the formation and maintenance of the nodes of Ranvier on myelinated axons. Nodes of Ranvier contain clustered sodium channels that are crucial for the saltatory propagation of action potentials along myelinated axons. During development, nodes of Ranvier are formed by the fusion of two heminodes. Required for normal clustering of sodium channels at heminodes; not required for the formation of mature nodes with normal sodium channel clusters. Required, together with GLDN, for maintaining NFASC and sodium channel clusters at mature nodes of Ranvier

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Cell projection, axon; Secreted

Expression：
Tissue_specificity:The substance was detected in all examined tissues. In the brain, it was detected in the amygdala, caudate nucleus, corpus callosum, hippocampus, hypothalamus, substantia nigra, subthalamic nucleus, and thalamus.

Isoforms & Post-Translational Modification：Q92823 has 6 isomers: Q92823-1: 143890 Da (predicted); Q92823-2: 136614 Da (predicted); Q92823-3: 130668 Da (predicted); Q92823-4: 131046 Da (predicted); Q92823-5: 144375 Da (predicted); Q92823-6: 131744 Da (predicted).

Subunit：Constituent of a NFASC/NRCAM/ankyrin-G complex. Detected in a complex with CNTN1 and PTPRB. Interacts with GLDN/gliomedin (By similarity). Interacts with MYOC (PubMed:23897819)

RRID

AB_3104799

Database

Entrez Gene: 4897 Human

SwissProt: Q92823 Human

OMIM: 619833 Human

Research Field

Neuroscience

Synonyms

Bravo; hBravo; Neuronal cell adhesion molecule; Ng CAM related; NgCAM related cell adhesion molecule; Nr CAM; Nrcam

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

NrCAM Antibody (YA2444) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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NrCAM Antibody (YA2444)

NrCAM Antibody (YA2444) Featured Recommendations:

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Background

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NrCAM Antibody (YA2444) Related Classifications

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