ERO1L Antibody (YA6558)

Cat. No.: HY-P86865: Data Sheet COA
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ERO1L Antibody (YA6558) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ERO1L.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation
References

Description

ERO1L Antibody (YA6558) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ERO1L.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 54 kDa;

Observed band size: 60 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q96HE7

Gene ID

30001 [NCBI]

Immunogen

Recombinant protein within human ERO1L aa 1-468.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:1000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HEK293 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and RH-35 (lane 4(20μg)) using ERO1L Antibody (HY-P86865) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. Required for the proper folding of immunoglobulins (PubMed:29858230). Plays an important role in ER stress-induced, CHOP-dependent apoptosis by activating the inositol 1,4,5-trisphosphate receptor IP3R1. Involved in the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin

Subcellular Localization：Endoplasmic reticulum membrane,Golgi apparatus lumen,Secreted,Cell projection, dendrite

Expression：
Tissue_Specificity: Widely expressed at low level. Expressed at high level in upper digestive tract. Highly expressed in esophagus. Weakly expressed in stomach and duodenum
Induction: Stimulated by hypoxia; suggesting that it is regulated via the HIF-pathway

Isoforms & Post-Translational Modification：Q96HE7: 468 amino acids, molecular weight 54393 Da.

Subunit：Predominantly monomer

Synonyms

Endoplasmic oxidoreductin 1 like protein antibody; Endoplasmic oxidoreductin-1-like protein antibody; Endoplasmic reticulum oxidoreductase 1 alpha antibody; Endoplasmic reticulum oxidoreductin 1-like antibody; ERO1 alpha antibody; ERO1 L antibody; ERO1 Lalpha antibody; ERO1 like protein alpha antibody; ERO1-alpha antibody; ERO1-L antibody; Endoplasmic oxidoreductin 1 like protein antibody; Endoplasmic oxidoreductin-1-like protein antibody; Endoplasmic reticulum oxidoreductase 1 alpha antibody; Endoplasmic reticulum oxidoreductin 1-like antibody; ERO1 alpha antibody; ERO1 L antibody; ERO1 Lalpha antibody; ERO1 like protein alpha antibody; ERO1-alpha antibody; ERO1-L antibody; ERO1-L-alpha antibody; ERO1-like (S. cerevisiae) antibody; ERO1-like alpha antibody; ERO1-like protein alpha antibody; ERO1-like, S. cerevisiae, homolog of, alpha antibody; ERO1A antibody; ERO1A_HUMAN antibody; ERO1L antibody; ERO1LA antibody; Oxidoreductin 1 Lalpha antibody; Oxidoreductin-1-L-alpha antibody; PRO865 antibody; UNQ434 antibody;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (254 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/lb2518-mel-cell.html