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ERO1L Antibody (YA6558)

(Synonyms: Endoplasmic oxidoreductin 1 like protein antibody; Endoplasmic oxidoreductin-1-like protein antibody; Endoplasmic reticulum oxidoreductase 1 alpha antibody; Endoplasmic reticulum oxidoreductin 1-like antibody; ERO1 alpha antibody; ERO1 L antibody; ERO1 Lalpha antibody; ERO1 like protein alpha antibody; ERO1-alpha antibody; ERO1-L antibody; Endoplasmic oxidoreductin 1 like protein antibody; Endoplasmic oxidoreductin-1-like protein antibody; Endoplasmic reticulum oxidoreductase 1 alpha antibody; Endoplasmic reticulum oxidoreductin 1-like antibody; ERO1 alpha antibody; ERO1 L antibody; ERO1 Lalpha antibody; ERO1 like protein alpha antibody; ERO1-alpha antibody; ERO1-L antibody; ERO1-L-alpha antibody; ERO1-like (S. cerevisiae) antibody; ERO1-like alpha antibody; ERO1-like protein alpha antibody; ERO1-like, S. cerevisiae, homolog of, alpha antibody; ERO1A antibody; ERO1A_HUMAN antibody; ERO1L antibody; ERO1LA antibody; Oxidoreductin 1 Lalpha antibody; Oxidoreductin-1-L-alpha antibody; PRO865 antibody; UNQ434 antibody; )
Customer Review

Based on 1 Customer Validation

ERO1L Antibody (YA6558) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ERO1L.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ERO1L Antibody (YA6558)
  • ERO1L Antibody (YA6558) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000 1:1000

Product Details

Description

ERO1L Antibody (YA6558) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ERO1L.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 60 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 54 kDa
Immunogen

Recombinant protein within human ERO1L aa 1-468.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ERO1L Antibody (YA6558)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HEK293 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and RH-35 (lane 4(20μg)) using ERO1L Antibody (HY-P86865) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    ERO1L is an Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. Required for the proper folding of immunoglobulins. Plays an important role in ER stress-induced, CHOP-dependent apoptosis by activating the inositol 1,4,5-trisphosphate receptor IP3R1. Involved in the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin[1].

  • Subcellular Localization

    Endoplasmic reticulum membrane,Golgi apparatus lumen,Secreted,Cell projection, dendrite

  • Expression


    Tissue_Specificity: Widely expressed at low level. Expressed at high level in upper digestive tract. Highly expressed in esophagus. Weakly expressed in stomach and duodenum
    Induction: Stimulated by hypoxia; suggesting that it is regulated via the HIF-pathway

  • Isoforms & Post-Translational Modification

    Q96HE7: 468 amino acids, molecular weight 54393 Da.

  • Subunit

    Predominantly monomer

  • SwissProt ID

    Q96HE7

  • Gene ID
    30001 [NCBI]
  • Synonyms

    Endoplasmic oxidoreductin 1 like protein antibody; Endoplasmic oxidoreductin-1-like protein antibody; Endoplasmic reticulum oxidoreductase 1 alpha antibody; Endoplasmic reticulum oxidoreductin 1-like antibody; ERO1 alpha antibody; ERO1 L antibody; ERO1 Lalpha antibody; ERO1 like protein alpha antibody; ERO1-alpha antibody; ERO1-L antibody; Endoplasmic oxidoreductin 1 like protein antibody; Endoplasmic oxidoreductin-1-like protein antibody; Endoplasmic reticulum oxidoreductase 1 alpha antibody; Endoplasmic reticulum oxidoreductin 1-like antibody; ERO1 alpha antibody; ERO1 L antibody; ERO1 Lalpha antibody; ERO1 like protein alpha antibody; ERO1-alpha antibody; ERO1-L antibody; ERO1-L-alpha antibody; ERO1-like (S. cerevisiae) antibody; ERO1-like alpha antibody; ERO1-like protein alpha antibody; ERO1-like, S. cerevisiae, homolog of, alpha antibody; ERO1A antibody; ERO1A_HUMAN antibody; ERO1L antibody; ERO1LA antibody; Oxidoreductin 1 Lalpha antibody; Oxidoreductin-1-L-alpha antibody; PRO865 antibody; UNQ434 antibody;

ERO1L Antibody (YA6558) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HEK293 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and RH-35 (lane 4(20μg)) using ERO1L Antibody (HY-P86865) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • ERO1L Antibody (YA6558) Product Image
    1/16
  • Experimental Validation Results for ERO1L Antibody (YA6558)
  • ERO1L Antibody (YA6558) Product Image
  • Experimental Validation Results for ERO1L Antibody (YA6558)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HEK293 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and RH-35 (lane 4(20μg)) using ERO1L Antibody (HY-P86865) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ERO1L Antibody (YA6558)
  • ERO1L Antibody (YA6558) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HEK293 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and RH-35 (lane 4(20μg)) using ERO1L Antibody (HY-P86865) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • ERO1L Antibody (YA6558) Product Image WB