Caspase-6 Antibody (YA1557)
(Synonyms: CASP6; MCH2; Caspase-6; CASP-6; Apoptotic protease Mch-2)Caspase-6 Antibody (YA1557) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Caspase-6.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human, Rat
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:500-1:1000 |
Product Details
Caspase-6 Antibody (YA1557) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Caspase-6.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Rat
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Observed Molecular WeightObserved band size: 11,33 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 33 kDa
Entrez Gene: 839 Human ; 12368 Mouse ;
SwissProt: P55212 Human ; O08738 Mouse ; O35397 Rat
OMIM: 601532 Human
Recombinant protein of human Caspase-6 aa194-293.
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Caspase-6 (CASP6) is a cysteine-aspartate protease traditionally classified as an executioner caspase that participates in apoptotic signaling and substrate cleavage during programmed cell death[1][2]. Mechanistically, caspase-6 functions within the broader caspase network that regulates apoptosis, inflammation, and cellular homeostasis, and accumulating evidence demonstrates that its biological activities extend beyond classical apoptotic execution[1][3]. In innate immune pathways, caspase-6 promotes inflammasome activation and facilitates the assembly of the ZBP1-PANoptosome, thereby supporting pyroptotic, apoptotic, and necroptotic signaling collectively termed PANoptosis[3]. During influenza A virus infection, caspase-6 enhances the interaction between RIPK3 and ZBP1, contributing to inflammatory cell death, cytokine production, and host defense responses[3]. Disease relevance is further supported by studies linking caspase-6 activity to neurodegenerative disorders, including Alzheimer’s disease and Huntington’s disease, where aberrant caspase-6 activation has been associated with pathological processes independent of overt apoptosis[2][4]. Compared with the closely related executioner caspases caspase-3 and caspase-7, caspase-6 displays distinct biological functions, including non-enzymatic regulation of innate immune signaling and PANoptosome-associated pathways, highlighting functional divergence within the executioner caspase subgroup[1][3]. For experimental applications, the development of isoform-selective caspase-6 inhibitors has attracted significant interest because selective modulation of caspase-6 may enable mechanistic studies while reducing off-target effects associated with broad-spectrum caspase inhibition[5].
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Subcellular Localization
Cytoplasm; Nucleus
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Isoforms & Post-Translational Modification
P55212 has 2 isomers: P55212-1: 33310 Da (predicted); P55212-2: 22574 Da (predicted).
Phosphorylated by NUAK1; phosphorylation inhibits self-activation (PubMed:15273717, PubMed:22483120). Phosphorylation at Ser-257 by AMP-activated protein kinase (PRKAA1 or PRKAA2) inhibits autocleavage, preventing caspase activation, thereby preventing hepatocyte apoptosis (PubMed:32029622);Palmitoylation by ZDHHC17 blocks dimerization and subsequent activation, leading to inhibit the cysteine protease activity;Can be cleaved and activated by different caspases, depending on the context (PubMed:19133298, PubMed:28864531). Cleaved and activated by caspase-8 (CASP8) and subsequently by caspase-3 (CASP3) (PubMed:9463409). Can also undergo autoactivation by mediating autocleavage at Asp-179 and Asp-193, while it is not able to cleave its N-terminal disordered prodomain (PubMed:19133298, PubMed:28864531). Cleaved and activated by CASP1, possibly in the context of inflammation (PubMed:16123779) -
Subunit
Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 11 kDa (p11) subunits (PubMed:19133298, PubMed:19694615, PubMed:20890311). Interacts with BIRC6/bruce (PubMed:15200957). Interacts with RIPK3 (PubMed:32298652)
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SwissProt ID
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Synonyms
CASP6; MCH2; Caspase-6; CASP-6; Apoptotic protease Mch-2
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Research Field
Cell Biology
Documentation
References
[1]. Nadendla EK, et al. Caspases: structural and molecular mechanisms and functions in cell death, innate immunity, and disease. Cell Discov. 2025 May 5;11(1):42. [Content Brief]
[2]. Wang XJ, et al. Activation and regulation of caspase-6 and its role in neurodegenerative diseases. Annu Rev Pharmacol Toxicol. 2015;55:553-72. [Content Brief]
[3]. Zheng M, et al. Caspase-6 Is a Key Regulator of Innate Immunity, Inflammasome Activation, and Host Defense. Cell. 2020 Apr 30;181(3):674-687.e13. [Content Brief]
[4]. Yang L, et al. ncRNA- and Pc2 methylation-dependent gene relocation between nuclear structures mediates gene activation programs. Cell. 2011 Nov 11;147(4):773-88. [Content Brief]
[5]. Van Horn KS, et al. Engaging a Non-catalytic Cysteine Residue Drives Potent and Selective Inhibition of Caspase-6. J Am Chem Soc. 2023 May 10;145(18):10015-10021. [Content Brief]
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