WWTR1 (TAZ) Antibody (YA7748)(PBS only)

Cat. No.: HY-P88064A: Data Sheet User Guide for Antibodies
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WWTR1 (TAZ) Antibody (YA7748) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to WWTR1 (TAZ).

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

WWTR1 (TAZ) Antibody (YA7748) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to WWTR1 (TAZ).

Host

Mouse

Molecular Weight

Predicted band size: 43.9 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9GZV5

Immunogen

Full length human recombinant protein of human WWTR1 produced in HEK293 cell.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:11118213, PubMed:18227151, PubMed:23911299). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18227151). WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation (PubMed:19010321). In conjunction with YAP1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (PubMed:18568018). Plays a key role in coupling SMADs to the transcriptional machinery such as the mediator complex (PubMed:18568018). Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition (PubMed:18227151, PubMed:18568018)

Subcellular Localization：Nucleus,Cytoplasm,Cell membrane,Cell junction, tight junction

Expression：
Tissue_Specificity: Highly expressed in kidney, heart, placenta and lung. Expressed in the thyroid tissue

Isoforms & Post-Translational Modification：Q9GZV5: 400 amino acids, molecular weight 44101 Da.

Subunit：Binds to SLC9A3R2 via the PDZ motif at the plasma membrane (By similarity)

Synonyms

TAZ

Documentation

User Guide for Antibodies (1077 KB)