Copy Link Print Information

MAP2 Antibody

(Synonyms: Microtubule associated protein 2; MAP2A; MAP2B; MAP2C)
Customer Review

Based on 1 Customer Validation

MAP2 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to MAP2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MAP2 Antibody
  • Experimental Validation Results for MAP2 Antibody
  • MAP2 Antibody Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
You May Still Need
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:2000 1:50-1:100 1:200-1:1000

Product Details

Description

MAP2 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to MAP2.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 300/70 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 200 kDa
Species Reactivity Database
Immunogen

Synthetic peptide within human MAP2 aa 1760-1810.

Sensitivity

Endogenous

Purification

Immunogen affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103044

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MAP2 Antibody
    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using MAP2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MAP2 Antibody
    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using MAP2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules

  • Subcellular Localization

    Cytoplasm, cytoskeleton; Cell projection, dendrite

  • Isoforms & Post-Translational Modification

    P11137 has 4 isomers: P11137-1: 199526 Da (predicted); P11137-2: 49640 Da (predicted); P11137-3: 199084 Da (predicted); P11137-4: 58954 Da (predicted).
    Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity)

  • Subunit

    Interacts with KNDC1 (via KIND2); the interaction enhances MAP2 phosphorylation and localizes KNDC1 to dendrites. Interacts with DPYSL5 (PubMed:33894126)

  • SwissProt ID

    P11137

  • Gene ID
    4133 [NCBI]
  • Synonyms

    Microtubule associated protein 2; MAP2A; MAP2B; MAP2C

  • Research Field

    Neuroscience

MAP2 Antibody Related Classifications

100 mg

Get Quote In-stock

Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MAP2 Antibody purchased from MedChemExpress. Usage Cited in:

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using MAP2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MAP2 Antibody purchased from MedChemExpress. Usage Cited in:

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using MAP2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • MAP2 Antibody Product Image
    1/16
  • Experimental Validation Results for MAP2 Antibody
  • Experimental Validation Results for MAP2 Antibody
  • MAP2 Antibody Product Image
  • MAP2 Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using MAP2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • MAP2 Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using MAP2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • MAP2 Antibody Product Image IHC-P
  • MAP2 Antibody Product Image IHC-P