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ERCC8 Antibody (YA2901)

(Synonyms: CKN1; CSA; ERCC8)
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Based on 1 Customer Validation

ERCC8 Antibody (YA2901) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ERCC8.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ERCC8 Antibody (YA2901)
  • ERCC8 Antibody (YA2901) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:5000 1:10-1:100

Product Details

Description

ERCC8 Antibody (YA2901) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ERCC8.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 44 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 44 kDa
Species Reactivity Database

Entrez Gene: 1161 Human

SwissProt: Q13216 Human

Immunogen

A synthetic peptide of human ERCC8

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ERCC8 Antibody (YA2901)
    Western blot analysis was performed on protein extracts (25 μg) from U87 (lane 2), 293T (lane 3), and HepG2 (lane 4) using ERCC8 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    ERCC8 is a Substrate-recognition component of the CSA complex, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair (TC-NER), a process during which RNA polymerase II-blocking lesions are rapidly removed from the transcribed strand of active genes. Following recruitment to lesion-stalled RNA polymerase II (Pol II), the CSA complex mediates ubiquitination of Pol II subunit POLR2A/RPB1 at 'Lys-1268', a critical TC-NER checkpoint, governing RNA Pol II stability and initiating DNA damage excision by TFIIH recruitment. The CSA complex also promotes the ubiquitination and subsequent proteasomal degradation of ERCC6/CSB in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair. Also plays a role in DNA double-strand breaks (DSSBs) repair by non-homologous end joining (NHEJ)[1][2][3][4][5][6][7][8][9][10].

  • Subcellular Localization

    Nucleus; Chromosome; Nucleus matrix

  • Isoforms & Post-Translational Modification

    Q13216 has 2 isomers: Q13216-1: 44055 Da (predicted); Q13216-2: 23182 Da (predicted).

  • Subunit

    Part of the CSA complex (also named DCX(ERCC8) complex), a DCX E3 ubiquitin-protein ligase complex containing ERCC8, RBX1, DDB1 and CUL4A; the CSA complex interacts with RNA polymerase II; upon UV irradiation it interacts with the COP9 signalosome and preferentially with the hyperphosphorylated form of RNA polymerase II (PubMed:12732143, PubMed:22118460, PubMed:32355176, PubMed:34526721, PubMed:38316879). Interacts with ERCC6/CSB (via CIM motif); promoting recruitment to lesion-stalled RNA polymerase II (Pol II) (PubMed:16751180, PubMed:32355176, PubMed:7664335). Interacts with KIAA1530/UVSSA (PubMed:22466612). Interacts with a subunit of RNA polymerase II TFIIH (PubMed:7664335)

  • SwissProt ID

    Q13216

  • Gene ID
    1161 [NCBI]
  • Synonyms

    CKN1; CSA; ERCC8

  • Research Field

    -

[1]. Groisman R, et al. The ubiquitin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage. Cell. 2003 May 2;113(3):357-67. [Content Brief]

[2]. Groisman R, et al. CSA-dependent degradation of CSB by the ubiquitin-proteasome pathway establishes a link between complementation factors of the Cockayne syndrome. Genes Dev. 2006 Jun 1;20(11):1429-34. [Content Brief]

[3]. Angers S, et al. Molecular architecture and assembly of the DDB1-CUL4A ubiquitin ligase machinery. Nature. 2006 Oct 5;443(7111):590-3. [Content Brief]

[4]. Nakazawa Y, et al. Ubiquitination of DNA Damage-Stalled RNAPII Promotes Transcription-Coupled Repair. Cell. 2020 Mar 19;180(6):1228-1244.e24. [Content Brief]

[5]. Kokic G, et al. Structural basis of human transcription-DNA repair coupling. Nature. 2021 Oct;598(7880):368-372. [Content Brief]

[6]. Kokic G, et al. Structural basis for RNA polymerase II ubiquitylation and inactivation in transcription-coupled repair. Nat Struct Mol Biol. 2024 Mar;31(3):536-547. [Content Brief]

[7]. Carnie CJ, et al. Transcription-coupled repair of DNA-protein cross-links depends on CSA and CSB. Nat Cell Biol. 2024 May;26(5):797-810. [Content Brief]

[8]. van Sluis M, et al. Transcription-coupled DNA-protein crosslink repair by CSB and CRL4(CSA)-mediated degradation. Nat Cell Biol. 2024 May;26(5):770-783. [Content Brief]

[9]. van der Weegen Y, et al. The cooperative action of CSB, CSA, and UVSSA target TFIIH to DNA damage-stalled RNA polymerase II. Nat Commun. 2020 Apr 30;11(1):2104. [Content Brief]

[10]. Pascucci B, et al. CSA and CSB play a role in the response to DNA breaks. Oncotarget. 2018 Feb 20;9(14):11581-11591. [Content Brief]

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ERCC8 Antibody (YA2901) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from U87 (lane 2), 293T (lane 3), and HepG2 (lane 4) using ERCC8 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • ERCC8 Antibody (YA2901) Product Image
    1/16
  • Experimental Validation Results for ERCC8 Antibody (YA2901)
  • ERCC8 Antibody (YA2901) Product Image
  • Experimental Validation Results for ERCC8 Antibody (YA2901)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from U87 (lane 2), 293T (lane 3), and HepG2 (lane 4) using ERCC8 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for ERCC8 Antibody (YA2901)
  • ERCC8 Antibody (YA2901) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from U87 (lane 2), 293T (lane 3), and HepG2 (lane 4) using ERCC8 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • ERCC8 Antibody (YA2901) Product Image WB