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MYO19 Antibody (YA3282)

(Synonyms: Myosin head domain-containing protein 1)
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Based on 1 Customer Validation

MYO19 Antibody (YA3282) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MYO19.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MYO19 Antibody (YA3282)
  • Experimental Validation Results for MYO19 Antibody (YA3282)
  • MYO19 Antibody (YA3282) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

MYO19 Antibody (YA3282) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MYO19.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 109 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 109 kDa
Species Reactivity Database

Entrez Gene: 80179 Human ;

SwissProt: Q96H55 Human ;

Immunogen

Recombinant protein of human MYO19

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105646

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MYO19 Antibody (YA3282)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), K562 (lane 2, 15 μg), 293T (lane 3, 15 μg), A549 (lane 4, 15 μg), 293 (lane 5, 15 μg), and 3T3 (lane 6, 15 μg) using MYO19 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:40000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for MYO19 Antibody (YA3282)
    Western blot analysis of extracts from K562 (lane 2(20μg), NIH3T3 (lane 3(20μg) and Hela (lane 4(20μg) using MYO19 (HY-P83537) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    MYO19 is an Actin-based motor molecule with ATPase activity that localizes to the mitochondrion outer membrane. Motor protein that moves towards the plus-end of actin filaments. Required for mitochondrial inheritance during mitosis. May be involved in mitochondrial transport or positioning[1][2][3].

  • Subcellular Localization

    Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasm, cytoskeleton

  • Expression


    Tissue_specificity:Widely expressed in multiple tissues and cell lines

  • Isoforms & Post-Translational Modification

    Q96H55 has 4 isomers: Q96H55-1: 109135 Da (predicted); Q96H55-2: 35934 Da (predicted); Q96H55-3: 71700 Da (predicted); Q96H55-4: 86687 Da (predicted).

  • Subunit

    Myosin is a hexamer of 2 heavy chains and 4 light chains: interacts with myosin light chains MYL9 and MYL12B

  • SwissProt ID

    Q96H55

  • Gene ID
    80179 [NCBI]
  • Synonyms

    Myosin head domain-containing protein 1

  • Research Field

    Signal Transduction

MYO19 Antibody (YA3282) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), K562 (lane 2, 15 μg), 293T (lane 3, 15 μg), A549 (lane 4, 15 μg), 293 (lane 5, 15 μg), and 3T3 (lane 6, 15 μg) using MYO19 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:40000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg), NIH3T3 (lane 3(20μg) and Hela (lane 4(20μg) using MYO19 (HY-P83537) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • MYO19 Antibody (YA3282) Product Image
    1/16
  • Experimental Validation Results for MYO19 Antibody (YA3282)
  • Experimental Validation Results for MYO19 Antibody (YA3282)
  • MYO19 Antibody (YA3282) Product Image
  • Experimental Validation Results for MYO19 Antibody (YA3282)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), K562 (lane 2, 15 μg), 293T (lane 3, 15 μg), A549 (lane 4, 15 μg), 293 (lane 5, 15 μg), and 3T3 (lane 6, 15 μg) using MYO19 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:40000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for MYO19 Antibody (YA3282)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg), NIH3T3 (lane 3(20μg) and Hela (lane 4(20μg) using MYO19 (HY-P83537) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MYO19 Antibody (YA3282)
  • Experimental Validation Results for MYO19 Antibody (YA3282)
  • MYO19 Antibody (YA3282) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), K562 (lane 2, 15 μg), 293T (lane 3, 15 μg), A549 (lane 4, 15 μg), 293 (lane 5, 15 μg), and 3T3 (lane 6, 15 μg) using MYO19 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:40000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • MYO19 Antibody (YA3282) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562 (lane 2(20μg), NIH3T3 (lane 3(20μg) and Hela (lane 4(20μg) using MYO19 (HY-P83537) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • MYO19 Antibody (YA3282) Product Image WB
  • MYO19 Antibody (YA3282) Product Image WB