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  5. MYO19 Antibody (YA3282)

MYO19 Antibody (YA3282) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MYO19.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

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제품 설명

MYO19 Antibody (YA3282) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MYO19.
Host

Rabbit
Clonality

Recombinant,Monoclonal
분자량
Predicted band size: 109 kDa;
Observed band size: 109 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse
SwissProt ID
Gene ID
Immunogen

Recombinant protein of human MYO19
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
선적

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), K562 (lane 2, 15 μg), 293T (lane 3, 15 μg), A549 (lane 4, 15 μg), 293 (lane 5, 15 μg), and 3T3 (lane 6, 15 μg) using MYO19 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:40000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Western blot analysis of extracts from K562 (lane 2(20μg), NIH3T3 (lane 3(20μg) and Hela (lane 4(20μg) using MYO19 (HY-P83537) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Function：Actin-based motor molecule with ATPase activity that localizes to the mitochondrion outer membrane (PubMed:19932026, PubMed:23568824, PubMed:25447992). Motor protein that moves towards the plus-end of actin filaments (By similarity). Required for mitochondrial inheritance during mitosis (PubMed:25447992). May be involved in mitochondrial transport or positioning (PubMed:23568824)
Subcellular Localization：Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasm, cytoskeleton
Expression：
Tissue_specificity:Widely expressed in multiple tissues and cell lines
Isoforms & Post-Translational Modification：Q96H55 has 4 isomers: Q96H55-1: 109135 Da (predicted); Q96H55-2: 35934 Da (predicted); Q96H55-3: 71700 Da (predicted); Q96H55-4: 86687 Da (predicted).
Subunit：Myosin is a hexamer of 2 heavy chains and 4 light chains: interacts with myosin light chains MYL9 and MYL12B
RRID
Database

Entrez Gene: 80179 Human ;

SwissProt: Q96H55 Human ;

OMIM: 617379 Human

Research Field

Signal Transduction
Synonyms
Myosin head domain-containing protein 1
각종 서류
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

MYO19 Antibody (YA3282) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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상품명:
MYO19 Antibody (YA3282)
Cat. No.:
HY-P83537
수량:
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SAFETY DATA SHEET (SDS)

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