CENPB Antibody (YA6952)

Cat. No.: HY-P87269: Data Sheet COA
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User Guide for Antibodies
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CENPB Antibody (YA6952) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to CENPB.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

사이즈	재고	수량
20 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
제품 설명

제품 설명

CENPB Antibody (YA6952) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to CENPB.

Host

Mouse

Clonality

Monoclonal

분자량

Predicted band size: 65 kDa;

Observed band size: 77 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P07199

Immunogen

Recombinant protein within human CENPB aa 1-200 / 599.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:100
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:500

Sensitivity	Endogenous	Purity	Protein G affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from A431 (lane 1(20μg)) 、MCF7 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using CENPB Antibody (HY-P87269) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Interacts with centromeric heterochromatin in chromosomes and binds to a specific 17 bp subset of alphoid satellite DNA, called the CENP-B box (PubMed:11726497). May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes (Probable)

Subcellular Localization：Nucleus,Chromosome, centromere

Isoforms & Post-Translational Modification：P07199: 599 amino acids, molecular weight 65171 Da.

Subunit：Antiparallel homodimer (PubMed:14522975, PubMed:1469042)

RRID

AB_3719299

Synonyms

CENP B antibody; CENP-B antibody; CENPB antibody; CENPB_HUMAN antibody; centromere autoantigen B antibody; Centromere protein B antibody; Centromere protein B, 80kDa antibody; Major centromere autoantigen B antibody

각종 서류

Select Batch:

Data Sheet (231 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)