Copy Link Print Information

CENPB Antibody (YA6952)

(Synonyms: CENP B antibody; CENP-B antibody; CENPB antibody; CENPB_HUMAN antibody; centromere autoantigen B antibody; Centromere protein B antibody; Centromere protein B, 80kDa antibody; Major centromere autoantigen B antibody)
Customer Review

Based on 1 Customer Validation

CENPB Antibody (YA6952) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to CENPB.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2a

  • Application:

    WB, ICC/IF, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CENPB Antibody (YA6952)
  • CENPB Antibody (YA6952) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:2000 1:50-1:100 1:100-1:500

Product Details

Description

CENPB Antibody (YA6952) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to CENPB.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 77 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 65 kDa
Immunogen

Recombinant protein within human CENPB aa 1-200 / 599.

Sensitivity

Endogenous

Purification

Protein G affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2a

RRID

AB_3719299

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CENPB Antibody (YA6952)
    Western blot analysis of extracts from A431 (lane 1(20μg)) 、MCF7 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using CENPB Antibody (HY-P87269) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    CENPB interacts with centromeric heterochromatin in chromosomes and binds to a specific 17 bp subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes (Probable)[1].

  • Subcellular Localization

    Nucleus,Chromosome, centromere

  • Isoforms & Post-Translational Modification

    P07199: 599 amino acids, molecular weight 65171 Da.

  • Subunit

    Antiparallel homodimer (PubMed:14522975, PubMed:1469042)

  • SwissProt ID

    P07199

  • Synonyms

    CENP B antibody; CENP-B antibody; CENPB antibody; CENPB_HUMAN antibody; centromere autoantigen B antibody; Centromere protein B antibody; Centromere protein B, 80kDa antibody; Major centromere autoantigen B antibody

CENPB Antibody (YA6952) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A431 (lane 1(20μg)) 、MCF7 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using CENPB Antibody (HY-P87269) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • CENPB Antibody (YA6952) Product Image
    1/16
  • Experimental Validation Results for CENPB Antibody (YA6952)
  • CENPB Antibody (YA6952) Product Image
  • Experimental Validation Results for CENPB Antibody (YA6952)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A431 (lane 1(20μg)) 、MCF7 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using CENPB Antibody (HY-P87269) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CENPB Antibody (YA6952)
  • CENPB Antibody (YA6952) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A431 (lane 1(20μg)) 、MCF7 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using CENPB Antibody (HY-P87269) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • CENPB Antibody (YA6952) Product Image WB