Growth hormone receptor Antibody

Cat. No.: HY-P81107: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Growth hormone receptor Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Growth hormone receptor.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Growth hormone receptor Antibody Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Growth hormone receptor Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Growth hormone receptor.

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 72 kDa;

Observed band size: 72 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P10912

Gene ID

2690 [NCBI]

Immunogen

Synthetic peptide of human GHR SKEPKFTKCRSPERET.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in pH 7.4 PBS, 0.05% NaN3, 40% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Raji (lane 2(20μg), Raji (lane 3(40μg), using Growth hormone receptor Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

Function：Receptor for pituitary gland growth hormone (GH1) involved in regulating postnatal body growth (PubMed:1549776, PubMed:2825030, PubMed:8943276). On ligand binding, couples to the JAK2/STAT5 pathway (PubMed:1549776, PubMed:15690087, PubMed:2825030, PubMed:8943276); The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling; Up-regulates the production of the soluble Growth hormone-binding protein form (GHBP) and acts as a negative inhibitor of growth hormone signaling

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Cell membrane; Single-pass type I membrane protein; Secreted

Expression：
Tissue_specificity:Expressed in various tissues with high expression in liver and skeletal muscle; Isoform 2 is expressed in lung, stomach and muscle; Predominantly expressed in kidney, bladder, adrenal gland and brain stem (PubMed:1569971) . Highly expressed in placental villi (PubMed:1569971, PubMed:8360189)

Isoforms & Post-Translational Modification：P10912 has 4 isomers: P10912-1: 71500 Da (predicted); P10912-2: 34109 Da (predicted); P10912-3: 33889 Da (predicted); P10912-4: 69237 Da (predicted).
The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE (PubMed:11785980). Shedding is inhibited by growth hormone (GH) binding to the receptor probably due to a conformational change in GHR rendering the receptor inaccessible to ADAM17 (By similarity);On GH binding, phosphorylated on tyrosine residues in the cytoplasmic domain by JAK2;Ubiquitinated by the ECS(SOCS2) complex following ligand-binding and phosphorylation by JAK2, leading to its degradation by the proteasome (PubMed:21980433, PubMed:25505247, PubMed:31182716, PubMed:34857742). Regulation by the ECS(SOCS2) complex acts as a negative feedback loop of growth hormone receptor signaling (PubMed:21980433). Ubiquitination is not sufficient for GHR internalization (By similarity)

Subunit：On growth hormone (GH) binding, forms homodimers and binds JAK2 via a box 1-containing domain

RRID

AB_3103213

Database

Entrez Gene: 2690 Human ; 14600 Mouse ; 25235 Rat

SwissProt: P10912 Human ; P16882 Mouse ; P16310 Rat

OMIM: 262500 Human

Synonyms

GH receptor; GHBP; GHR; Growth hormone receptor precursor; Serum binding protein; Somatotropin receptor; GHR_HUMAN.

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Growth hormone receptor Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Growth hormone receptor Antibody

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Growth hormone receptor Antibody Related Classifications

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