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Growth hormone receptor Antibody

(Synonyms: GH receptor; GHBP; GHR; Growth hormone receptor precursor; Serum binding protein; Somatotropin receptor; GHR_HUMAN.)
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Based on 1 Customer Validation

Growth hormone receptor Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Growth hormone receptor.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in pH 7.4 PBS, 0.05% NaN3, 40% Glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Growth hormone receptor Antibody
  • Growth hormone receptor Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Growth hormone receptor Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Growth hormone receptor.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 72 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 72 kDa
Species Reactivity Database
Immunogen

Synthetic peptide of human GHR SKEPKFTKCRSPERET.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103213

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in pH 7.4 PBS, 0.05% NaN3, 40% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Growth hormone receptor Antibody
    Western blot analysis of extracts from Raji (lane 2(20μg), Raji (lane 3(40μg), using Growth hormone receptor Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Growth hormone receptor receptor for pituitary gland growth hormone (GH1) involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway; The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling; Up-regulates the production of the soluble Growth hormone-binding protein form (GHBP) and acts as a negative inhibitor of growth hormone signaling[1][2][3][4].

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Cell membrane; Single-pass type I membrane protein; Secreted

  • Expression


    Tissue_specificity:Expressed in various tissues with high expression in liver and skeletal muscle; Isoform 2 is expressed in lung, stomach and muscle; Predominantly expressed in kidney, bladder, adrenal gland and brain stem (PubMed:1569971) . Highly expressed in placental villi (PubMed:1569971, PubMed:8360189)

  • Isoforms & Post-Translational Modification

    P10912 has 4 isomers: P10912-1: 71500 Da (predicted); P10912-2: 34109 Da (predicted); P10912-3: 33889 Da (predicted); P10912-4: 69237 Da (predicted).
    The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE (PubMed:11785980). Shedding is inhibited by growth hormone (GH) binding to the receptor probably due to a conformational change in GHR rendering the receptor inaccessible to ADAM17 (By similarity);On GH binding, phosphorylated on tyrosine residues in the cytoplasmic domain by JAK2;Ubiquitinated by the ECS(SOCS2) complex following ligand-binding and phosphorylation by JAK2, leading to its degradation by the proteasome (PubMed:21980433, PubMed:25505247, PubMed:31182716, PubMed:34857742). Regulation by the ECS(SOCS2) complex acts as a negative feedback loop of growth hormone receptor signaling (PubMed:21980433). Ubiquitination is not sufficient for GHR internalization (By similarity)

  • Subunit

    On growth hormone (GH) binding, forms homodimers and binds JAK2 via a box 1-containing domain

  • SwissProt ID

    P10912

  • Gene ID
    2690 [NCBI]
  • Synonyms

    GH receptor; GHBP; GHR; Growth hormone receptor precursor; Serum binding protein; Somatotropin receptor; GHR_HUMAN.

Growth hormone receptor Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Raji (lane 2(20μg), Raji (lane 3(40μg), using Growth hormone receptor Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Growth hormone receptor Antibody Product Image
    1/16
  • Experimental Validation Results for Growth hormone receptor Antibody
  • Growth hormone receptor Antibody Product Image
  • Experimental Validation Results for Growth hormone receptor Antibody
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Raji (lane 2(20μg), Raji (lane 3(40μg), using Growth hormone receptor Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Growth hormone receptor Antibody
  • Growth hormone receptor Antibody Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Raji (lane 2(20μg), Raji (lane 3(40μg), using Growth hormone receptor Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Growth hormone receptor Antibody Product Image WB