Phospho-RICTOR (Thr1135) Antibody (YA9810)
(Synonyms: PIA; mAVO3; KIAA1999; MGC39830; DKFZp686B11164; RICTOR)Based on 1 Customer Validation
Phospho-RICTOR (Thr1135) Antibody (YA9810) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Phospho-RICTOR (Thr1135).
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:2000 |
Product Details
Phospho-RICTOR (Thr1135) Antibody (YA9810) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Phospho-RICTOR (Thr1135).
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Host Rabbit
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 240 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 192 kDa
Synthetic phospho-peptide corresponding to residues surrounding Thr1135 of Human RICTOR.
Endogenous
affinity purified
Non-conjugated
Phosphorylated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Sodium Azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
RICTOR is a Component of the mechanistic target of rapamycin complex 2 (mTORC2), which transduces signals from growth factors to pathways involved in proliferation, cytoskeletal organization, lipogenesis and anabolic output. In response to growth factors, mTORC2 phosphorylates and activates AGC protein kinase family members, including AKT (AKT1, AKT2 and AKT3), PKC (PRKCA, PRKCB and PRKCE) and SGK1. In contrast to mTORC1, mTORC2 is nutrient-insensitive. Within the mTORC2 complex, RICTOR probably acts as a molecular adapter. RICTOR is responsible for the FKBP12-rapamycin-insensitivity of mTORC2. mTORC2 plays a critical role in AKT1 activation by mediating phosphorylation of different sites depending on the context, such as 'Thr-450', 'Ser-473', 'Ser-477' or 'Thr-479', facilitating the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDPK1/PDK1 which is a prerequisite for full activation. mTORC2 catalyzes the phosphorylation of SGK1 at 'Ser-422' and of PRKCA on 'Ser-657' (By similarity). The mTORC2 complex also phosphorylates various proteins involved in insulin signaling, such as FBXW8 and IGF2BP1 (By similarity). mTORC2 acts upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin[1][2][3][4][5][6][7][8][9][10].
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Subcellular Localization
Cell membrane; Endoplasmic reticulum membrane; Lysosome membrane
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Isoforms & Post-Translational Modification
Phospho-RICTOR has 3 isoforms, Q6R327-1: amino acid length is 1708, molecular weight is 192218 Da (predicted); Q6R327-4: amino acid length is 257, molecular weight is 28988 Da (predicted); Q6R327-3: amino acid length is 1732, molecular weight is 195011 Da (predicted).
Phosphorylated by MTOR; when part of mTORC2. Phosphorylated at Thr-1135 by RPS6KB1 downstream of the mTORC1 complex: phosphorylation of RICTOR inhibits mTORC2 signaling by creating a binding site for 14-3-3 proteins. Phosphorylated at Thr-1695 by GSK3A and GSK3B which facilitates RICTOR ubiquitination and subsequent degradation. Phosphorylated at Ser-1235 by GSK3B in response to endoplasmic stress, inhibiting mTORC2 signaling. -
Subunit
Component of the mechanistic target of rapamycin complex 2 (mTORC2), consisting in two heterotretramers composed of MTOR, MLST8, RICTOR and MAPKAP1/SIN1.
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SwissProt ID
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Synonyms
PIA; mAVO3; KIAA1999; MGC39830; DKFZp686B11164; RICTOR
Documentation
References
[1]. Sarbassov DD, et al. Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton. Curr Biol. 2004 Jul 27;14(14):1296-302. [Content Brief]
[2]. Sarbassov DD, et al. Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Science. 2005 Feb 18;307(5712):1098-101. [Content Brief]
[3]. Dibble CC, et al. Characterization of Rictor phosphorylation sites reveals direct regulation of mTOR complex 2 by S6K1. Mol Cell Biol. 2009 Nov;29(21):5657-70. [Content Brief]
[4]. Julien LA, et al. mTORC1-activated S6K1 phosphorylates Rictor on threonine 1135 and regulates mTORC2 signaling. Mol Cell Biol. 2010 Feb;30(4):908-21. [Content Brief]
[5]. Chen CH, et al. ER stress inhibits mTORC2 and Akt signaling through GSK-3β-mediated phosphorylation of rictor. Sci Signal. 2011 Feb 22;4(161):ra10. [Content Brief]
[6]. Scaiola A, et al. The 3.2-Å resolution structure of human mTORC2. Sci Adv. 2020 Nov;6(45):. [Content Brief]
[7]. Zhuang L, et al. TSPAN8 alleviates high glucose-induced apoptosis and autophagy via targeting mTORC2. Cell Biol Int. 2022 Oct;46(10):1693-1703. [Content Brief]
[8]. Yu Z, et al. Interactions between mTORC2 core subunits Rictor and mSin1 dictate selective and context-dependent phosphorylation of substrate kinases SGK1 and Akt. J Biol Chem. 2022 Sep;298(9):102288. [Content Brief]
[9]. Treins C, et al. Rictor is a novel target of p70 S6 kinase-1. Oncogene. 2010 Feb 18;29(7):1003-16. [Content Brief]
[10]. Jacinto E, et al. Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive. Nat Cell Biol. 2004 Nov;6(11):1122-8. [Content Brief]
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