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NEK6 Antibody (YA2396)

(Synonyms: NEK6; NimA related protein kinase 6; SID61512)
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Based on 1 Customer Validation

NEK6 Antibody (YA2396) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NEK6.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for NEK6 Antibody (YA2396)
  • Experimental Validation Results for NEK6 Antibody (YA2396)
  • NEK6 Antibody (YA2396) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:2000 1:50

Product Details

Description

NEK6 Antibody (YA2396) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NEK6.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 36-39 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 36-39 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human NEK6 aa250-313.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in rabbit IgG in 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NEK6 Antibody (YA2396)
    Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), HeLa (lane 3), HT-1080 (lane 4), 293T (lane 5), MCF-7 (lane 6), and Caco-2 (lane 7) using NEK6 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for NEK6 Antibody (YA2396)
    Western blot analysis of extracts from THP-1 (lane 2, 20ug), Hela (lane 3, 20ug) and C6 (lane 4, 20ug) using NEK6 Antibody (HY-P82651). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    NIMA-related kinase 6 (NEK6) is a serine/threonine kinase that regulates cell cycle progression, mitotic spindle assembly, and microtubule dynamics[1][2]. Mechanistically, NEK6 interacts with Smad4 to inhibit TGFβ/Smad-mediated transcription, blocking nuclear translocation and counteracting growth arrest in tumor models[3]. NEK6 also contributes to endocytic trafficking by regulating early and recycling endosome morphology and cargo sorting, a function distinct from NEK7 which shares partial overlapping roles[2]. In disease contexts, NEK6 mediates castration-resistant prostate cancer by maintaining androgen-independent proliferation and activating transcription factors such as FOXJ2[4]. In neurodegenerative models, NEK6 modulates poly (PR) -induced p53-related DNA damage in C9orf72-associated frontotemporal dementia and ALS, indicating its broader relevance beyond oncology[5]. Compared with related isoforms, NEK6 exhibits unique substrate specificity, particularly for Y-box binding protein-1 (YBX1) and Smad4, and forms protein complexes that localize at centrosomes and cytoplasmic networks[1][2][4]. Preclinical studies suggest that inhibition of NEK6, alone or combined with CDK4/6 inhibitors, effectively suppresses tumor growth and rescues cellular toxicity in neuronal models, highlighting its potential as a targetable kinase[5][6]. Therefore, NEK6 serves as a multifunctional regulator of mitotic control, intracellular trafficking, and stress responses, with implications for cancer therapy and neurodegenerative disease research[1][2][3][4][5][6].

  • Subcellular Localization

    Cytoplasm; Nucleus; Nucleus speckle; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cytoskeleton, spindle pole

  • Expression


    Tissue_specificity:Ubiquitous, with highest expression in heart and skeletal muscle

    Induction:Up-regulated during the M phase of cell cycle progression. Down-regulated in both replicative and premature senescence of cancer cells

  • Isoforms & Post-Translational Modification

    Q9HC98 has 4 isomers: Q9HC98-1: 35714 Da (predicted); Q9HC98-2: 39844 Da (predicted); Q9HC98-3: 37823 Da (predicted); Q9HC98-4: 38460 Da (predicted).
    Autophosphorylated. Phosphorylation at Ser-206 is required for its activation. Phosphorylated upon IR or UV-induced DNA damage. Phosphorylated by CHEK1 and CHEK2. Interaction with APBB1 down-regulates phosphorylation at Thr-210

  • Subunit

    Interacts with STAT3 and RPS6KB1 (By similarity). Interacts with NEK9, predominantly in mitosis. Interacts with KIF11 (via C-terminus). Interacts with APBB1 (via WW domain). Interacts with ANKRA2, ATF4, ARHGAP33, CDC42, CIR1, PRAM1, PTN, PRDX3, PIN1, RAD26L, RBBP6, RPS7 and TRIP4

  • SwissProt ID

    Q9HC98

  • Gene ID
    10783 [NCBI]
  • Synonyms

    NEK6; NimA related protein kinase 6; SID61512

  • Research Field

    Cell Biology

References

[1]. Vaz Meirelles G, et al. Characterization of hNek6 interactome reveals an important role for its short N-terminal domain and colocalization with proteins at the centrosome. J Proteome Res. 2010 Dec 3;9(12):6298-316. [Content Brief]

[2]. Joseph BB, et al. Conserved NIMA kinases regulate multiple steps of endocytic trafficking. PLoS Genet. 2023 Apr 26;19(4):e1010741. [Content Brief]

[3]. Zuo J, et al. An inhibitory role of NEK6 in TGFβ/Smad signaling pathway. BMB Rep. 2015 Aug;48(8):473-8. [Content Brief]

[4]. Choudhury AD, et al. Castration Resistance in Prostate Cancer Is Mediated by the Kinase NEK6. Cancer Res. 2017 Feb 1;77(3):753-765. [Content Brief]

[5]. Guo W, et al. CRISPR/Cas9 screen in human iPSC-derived cortical neurons identifies NEK6 as a novel disease modifier of C9orf72 poly(PR) toxicity. Alzheimers Dement. 2023 Apr;19(4):1245-1259. [Content Brief]

[6]. Jian F, et al. Genome-wide CRISPR screen identified NEK6 as a determinant of sensitivity to CDK4/6 inhibitor in endometrial cancer. Front Pharmacol. 2026 Jan 5;16:1725886. [Content Brief]

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NEK6 Antibody (YA2396) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), HeLa (lane 3), HT-1080 (lane 4), 293T (lane 5), MCF-7 (lane 6), and Caco-2 (lane 7) using NEK6 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from THP-1 (lane 2, 20ug), Hela (lane 3, 20ug) and C6 (lane 4, 20ug) using NEK6 Antibody (HY-P82651). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • NEK6 Antibody (YA2396) Product Image
    1/16
  • Experimental Validation Results for NEK6 Antibody (YA2396)
  • Experimental Validation Results for NEK6 Antibody (YA2396)
  • NEK6 Antibody (YA2396) Product Image
  • Experimental Validation Results for NEK6 Antibody (YA2396)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), HeLa (lane 3), HT-1080 (lane 4), 293T (lane 5), MCF-7 (lane 6), and Caco-2 (lane 7) using NEK6 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for NEK6 Antibody (YA2396)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from THP-1 (lane 2, 20ug), Hela (lane 3, 20ug) and C6 (lane 4, 20ug) using NEK6 Antibody (HY-P82651). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for NEK6 Antibody (YA2396)
  • Experimental Validation Results for NEK6 Antibody (YA2396)
  • NEK6 Antibody (YA2396) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), HeLa (lane 3), HT-1080 (lane 4), 293T (lane 5), MCF-7 (lane 6), and Caco-2 (lane 7) using NEK6 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • NEK6 Antibody (YA2396) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from THP-1 (lane 2, 20ug), Hela (lane 3, 20ug) and C6 (lane 4, 20ug) using NEK6 Antibody (HY-P82651). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • NEK6 Antibody (YA2396) Product Image WB
  • NEK6 Antibody (YA2396) Product Image WB