Nucleophosmin Antibody (YA1008)
(Synonyms: B23; NPM)Nucleophosmin Antibody (YA1008) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Nucleophosmin.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, ChIP
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
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Conjugation:
Non-conjugated
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ChIP
ChIP: Chromatin Immunoprecipitation
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|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:20 |
Product Details
Nucleophosmin Antibody (YA1008) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Nucleophosmin.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 38 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 33 kDa
Entrez Gene: 4869 Human ; 18148 Mouse ; 25498 Rat
SwissProt: P06748 Human ; Q61937 Mouse ; P13084 Rat
OMIM: 164040 Human
Purified recombinant full length of human NPM1 protein expressed in E.coli.
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Nucleophosmin is involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade. In complex with MYC enhances the transcription of MYC target genes. May act as chaperonin or cotransporter in the nucleolar localization of transcription termination factor TTF1[1][2].
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Subcellular Localization
Nucleus, nucleolus; Nucleus, nucleoplasm; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
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Isoforms & Post-Translational Modification
P06748 has 3 isomers: P06748-1: 32575 Da (predicted); P06748-2: 29465 Da (predicted); P06748-3: 28400 Da (predicted).
Acetylated at C-terminal lysine residues, thereby increasing affinity to histones;ADP-ribosylated;Phosphorylated at Ser-4 by PLK1 and PLK2. Phosphorylation at Ser-4 by PLK2 in S phase is required for centriole duplication and is sufficient to trigger centriole replication. Phosphorylation at Ser-4 by PLK1 takes place during mitosis. Phosphorylated by CDK2 at Ser-125 and Thr-199. Phosphorylation at Thr-199 may trigger initiation of centrosome duplication. Phosphorylated by CDK1 at Thr-199, Thr-219, Thr-234 and Thr-237 during cell mitosis. When these four sites are phosphorated, RNA-binding activity seem to be abolished. May be phosphorylated at Ser-70 by NEK2. The Thr-199 phosphorylated form has higher affinity for ROCK2. CDK6 triggers Thr-199 phosphorylation when complexed to Kaposi's sarcoma herpesvirus (KSHV) V-cyclin, leading to viral reactivation by reducing viral LANA levels;Sumoylated by ARF;Ubiquitinated. Ubiquitination leads to proteasomal degradation. Deubiquitinated by USP36 (PubMed:19208757) -
Subunit
Disulfide-linked dimers under certain conditions (PubMed:25818168).
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SwissProt ID
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Synonyms
B23; NPM
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Research Field
Epigenetics and Nuclear Signaling
Documentation
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