Cyclin D3/CCND3 Antibody (YA4807)
(Synonyms: CCND3; G1/S-specific cyclin-D3)Cyclin D3/CCND3 Antibody (YA4807) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Cyclin D3/CCND3.
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Host:
Mouse
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Application:
WB, ELISA
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:10000 |
Product Details
Cyclin D3/CCND3 Antibody (YA4807) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Cyclin D3/CCND3.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse
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Calculated Molecular Weight Predicted band size: 33 kDa;
Purified recombinant fragment of human Cyclin D3 expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Cyclin D3/CCND3 is a Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Shows transcriptional coactivator activity with ATF5 independently of CDK4[1][2][3].
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Subcellular Localization
Nucleus; Cytoplasm
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Isoforms & Post-Translational Modification
P30281 has 4 isomers: P30281-1: 32520 Da (predicted); P30281-2: 22887 Da (predicted); P30281-3: 24238 Da (predicted); P30281-4: 9736 Da (predicted).
Phosphorylation at Thr-283 by MAP kinases is required for ubiquitination and degradation by the DCX(AMBRA1) complex;Ubiquitinated by the DCX(AMBRA1) complex during the transition from G1 to S cell phase, leading to its degradation: ubiquitination is dependent on Thr-283 phosphorylation (PubMed:33854235, PubMed:33854239). The DCX(AMBRA1) complex represents the major regulator of CCND3 stability during the G1/S transition (PubMed:33854235, PubMed:33854239). Polyubiquitinated by the SCF(FBXL2) complex, leading to proteasomal degradation (PubMed:22020328) -
Subunit
Interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex (PubMed:19237555, PubMed:8114739, PubMed:9106657). The cyclin subunit imparts substrate specificity to the complex (PubMed:8114739). Interacts with ATF5 (PubMed:15358120). Interacts with EIF3K (PubMed:15327989). Component of the ternary complex cyclin D/CDK4/CDKN1B required for nuclear translocation and modulation of CDK4-mediated kinase activity (PubMed:16782892). Can form similar complexes with either CDKN1A or CDKN2A (PubMed:16782892, PubMed:9106657)
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SwissProt ID
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Synonyms
CCND3; G1/S-specific cyclin-D3
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