FH Antibody (YA5353)

Cat. No.: HY-P85661: Data Sheet User Guide for Antibodies
FAQs
Technical Support

FH Antibody (YA5353) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to FH.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명

제품 설명

FH Antibody (YA5353) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to FH.

Host

Mouse

Clonality

Monoclonal

분자량

Observed band size: 50 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P07954

Gene ID

2271 [NCBI]

Immunogen

Synthetic Peptide of FH

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:3000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-300

Purity	affinity chromatography.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：Catalyzes the reversible stereospecific interconversion of fumarate to L-malate (PubMed:30761759). Experiments in other species have demonstrated that specific isoforms of this protein act in defined pathways and favor one direction over the other (Probable); Catalyzes the hydration of fumarate to L-malate in the tricarboxylic acid (TCA) cycle to facilitate a transition step in the production of energy in the form of NADH; Catalyzes the dehydration of L-malate to fumarate (By similarity). Fumarate metabolism in the cytosol plays a role during urea cycle and arginine metabolism; fumarate being a by-product of the urea cycle and amino-acid catabolism (By similarity). Also plays a role in DNA repair by promoting non-homologous end-joining (NHEJ) (PubMed:20231875, PubMed:26237645). In response to DNA damage and phosphorylation by PRKDC, translocates to the nucleus and accumulates at DNA double-strand breaks (DSBs): acts by catalyzing formation of fumarate, an inhibitor of KDM2B histone demethylase activity, resulting in enhanced dimethylation of histone H3 'Lys-36' (H3K36me2) (PubMed:26237645)

Subcellular Localization：Mitochondrion; Cytoplasm, cytosol; Nucleus; Chromosome

Expression：
Tissue_specificity:It is expressed in erythrocytes; however, it is underexpressed in the cytoplasm of erythrocytes from hereditary nonspherocytic hemolytic anemia of unknown etiology.

Isoforms & Post-Translational Modification：P07954 has 2 isomers: P07954-1: 54637 Da (predicted); P07954-2: 50213 Da (predicted).
Phosphorylation at Thr-236 by PRKDC in response to DNA damage promotes translocation to the nucleus and recruitment to DNA double-strand breaks (DSBs)

Subunit：Homotetramer (PubMed:21445611, PubMed:30761759). Interacts with H2AZ1 (PubMed:26237645)

Synonyms

Fumarate hydratase, mitochondrial; Fumarase;

각종 서류

User Guide for Antibodies (1077 KB)