Heparanase 1 Antibody (YA1601)

製品番号: HY-P81856: データシート SDS COA User Guide for Antibodies
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Heparanase 1 Antibody (YA1601) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Heparanase 1.

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容量	価格（税別）	在庫状況	数量
無料サンプル		今すぐ申し込む
10 μL	$70	在庫あり	Estimated Time of Arrival: December 31
50 μL	$185	在庫あり	Estimated Time of Arrival: December 31
100 μL	$300	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明

製品説明

Heparanase 1 Antibody (YA1601) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Heparanase 1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

分子量

Predicted band size: 61 kDa;

Observed band size: 65 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9Y251

Gene ID

10855 [NCBI]

Immunogen

A synthesized peptide derived from human Heparanase 1 aa1-60/543.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:50

Sensitivity	Endogenous	純度	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Jurkat (lane 2(20μg), HeLa (lane 3(20μg), HepG2 (lane 4(20μg) and 293T (lane 5(20μg) using Heparanase 1 Antibody (HY-P81856) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as a procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extracellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis

Subcellular Localization：Lysosome membrane; Peripheral membrane protein; Secreted; Nucleus

Expression：
Tissue_specificity:Highly expressed in placenta and spleen and weakly expressed in lymph node, thymus, peripheral blood leukocytes, bone marrow, endothelial cells, fetal liver and tumor tissues. Also expressed in hair follicles, specifically in both Henle's and Huxley's layers of inner the root sheath (IRS) at anagen phase

Isoforms & Post-Translational Modification：Q9Y251 has 4 isomers: Q9Y251-1: 61149 Da (predicted); Q9Y251-2: 54734 Da (predicted); Q9Y251-3: 53161 Da (predicted); Q9Y251-4: 42791 Da (predicted).
Proteolytically processed. The cleavage of the 65 kDa form leads to the generation of a linker peptide, and 8 kDa and 50 kDa products. The active form, the 8/50 kDa heterodimer, is resistant to degradation. Complete removal of the linker peptide appears to be a prerequisite to the complete activation of the enzyme;N-glycosylated. Glycosylation of the 50 kDa subunit appears to be essential for its solubility

Subunit：Heterodimer; heterodimer formation between the 8 kDa and the 50 kDa subunits is required for enzyme activity. Interacts with TF; the interaction, inhibited by heparin, enhances the generation of activated factor X and activates coagulation. Interacts with HRG; the interaction is enhanced at acidic pH, partially inhibits binding of HPSE to cell surface receptors and modulates its enzymatic activity. Interacts with SDC1; the interaction enhances the shedding of SDC1. Interacts with HPSE2

RRID

AB_3103954

Database

Entrez Gene: 10855 Human ; 15442 Mouse ; 64537 Rat

SwissProt: Q9Y251 Human ; Q6YGZ1 Mouse ; Q71RP1 Rat

OMIM: 604724 Human

Research Field

Tags & Cell Markers

Synonyms

HEP; Heparanase; Heparanase1; Hpa 1; HPA; Hpa1 HPR1; HPSE1; HSE1

ドキュメンテーション

Select Batch:

データシート (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)