SNAI1 Antibody (YA5064)
(Synonyms: SNAI1; SNAH; Zinc finger protein SNAI1; Protein snail homolog 1; Protein sna)SNAI1 Antibody (YA5064) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to SNAI1.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human
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Formulation:
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:500-2000 |
Product Details
SNAI1 Antibody (YA5064) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to SNAI1.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 29 kDa;
Recombinant human SNAI1. The exact sequence is proprietary to MCE.
Endogenous
affinity purified by Protein G
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
SNAI1 is involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. The N-terminal SNAG domain competes with histone H3 for the same binding site on the histone demethylase complex formed by KDM1A and RCOR1, and thereby inhibits demethylation of histone H3 at 'Lys-4' (in vitro). During EMT, involved with LOXL2 in negatively regulating pericentromeric heterochromatin transcription. SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself[1][2][3][4][5][6][7][8][9][10].
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Subcellular Localization
Nucleus; Cytoplasm
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Expression
Tissue_specificity:It is expressed in a variety of tissues, with the highest expression in the kidney. It is also expressed in both mesenchymal cells and epithelial cell lines.
Induction:Induced by TPA maximally by 2.5-fold at 4 hours, in HepG2 cells (at protein level) -
Subunit
Interacts (via SNAG domain) with WTIP (via LIM domains) (By similarity). Interacts (via SNAG domain) with LIMD1 (via LIM domains), and AJUBA (via LIM domains) (PubMed:18331720). Interacts with LOXL2 and LOXL3 (PubMed:16096638).
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SwissProt ID
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Synonyms
SNAI1; SNAH; Zinc finger protein SNAI1; Protein snail homolog 1; Protein sna
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