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MTHFR Antibody (YA3258)

(Synonyms: MTHFR; Methylenetetrahydrofolate reductase)
Customer Review

Based on 1 Customer Validation

MTHFR Antibody (YA3258) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MTHFR.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for MTHFR Antibody (YA3258)
  • Experimental Validation Results for MTHFR Antibody (YA3258)
  • Experimental Validation Results for MTHFR Antibody (YA3258)
  • MTHFR Antibody (YA3258) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:20

Product Details

Description

MTHFR Antibody (YA3258) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MTHFR.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 76 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 75 kDa
Species Reactivity Database
Immunogen

Recombinant protein of human MTHFR

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105623

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MTHFR Antibody (YA3258)
    Western blot analysis of extracts from HEK293 (lane2, 20μg), K562 (lane3, 20μg), RAW264.7 (lane4, 20μg) and Jurkat (lane5, 20μg) using MTHFR Antibody (HY-P83513). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MTHFR Antibody (YA3258)
    Immunocytochemistry analysis of Hela cells labeling MTHFR with MTHFR Antibody (HY-P83513) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MTHFR Antibody (HY-P83513) at 1/50dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MTHFR Antibody (YA3258)
    Immunocytochemistry analysis of Hela cells labeling MTHFR with MTHFR Antibody (HY-P83513) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MTHFR Antibody (HY-P83513) at 1/100dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    MTHFR catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a cosubstrate for homocysteine remethylation to methionine. Represents a key regulatory connection between the folate and methionine cycles (Probable)[1].

  • Isoforms & Post-Translational Modification

    P42898 has 2 isomers: P42898-1: 74597 Da (predicted); P42898-2: 78965 Da (predicted).
    Phosphorylation of an N-terminal serine-rich phosphorylation region increases sensitivity to S-adenosylmethionine and inhibition

  • Subunit

    Homodimer

  • SwissProt ID

    P42898

  • Gene ID
    4524 [NCBI]
  • Synonyms

    MTHFR; Methylenetetrahydrofolate reductase

  • Research Field

    Signal Transduction

MTHFR Antibody (YA3258) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane2, 20μg), K562 (lane3, 20μg), RAW264.7 (lane4, 20μg) and Jurkat (lane5, 20μg) using MTHFR Antibody (HY-P83513). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling MTHFR with MTHFR Antibody (HY-P83513) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MTHFR Antibody (HY-P83513) at 1/50dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling MTHFR with MTHFR Antibody (HY-P83513) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MTHFR Antibody (HY-P83513) at 1/100dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • MTHFR Antibody (YA3258) Product Image
    1/16
  • Experimental Validation Results for MTHFR Antibody (YA3258)
  • Experimental Validation Results for MTHFR Antibody (YA3258)
  • Experimental Validation Results for MTHFR Antibody (YA3258)
  • MTHFR Antibody (YA3258) Product Image
  • Experimental Validation Results for MTHFR Antibody (YA3258)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane2, 20μg), K562 (lane3, 20μg), RAW264.7 (lane4, 20μg) and Jurkat (lane5, 20μg) using MTHFR Antibody (HY-P83513). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MTHFR Antibody (YA3258)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling MTHFR with MTHFR Antibody (HY-P83513) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MTHFR Antibody (HY-P83513) at 1/50dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MTHFR Antibody (YA3258)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling MTHFR with MTHFR Antibody (HY-P83513) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MTHFR Antibody (HY-P83513) at 1/100dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MTHFR Antibody (YA3258)
  • Experimental Validation Results for MTHFR Antibody (YA3258)
  • Experimental Validation Results for MTHFR Antibody (YA3258)
  • MTHFR Antibody (YA3258) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293 (lane2, 20μg), K562 (lane3, 20μg), RAW264.7 (lane4, 20μg) and Jurkat (lane5, 20μg) using MTHFR Antibody (HY-P83513). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • MTHFR Antibody (YA3258) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling MTHFR with MTHFR Antibody (HY-P83513) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MTHFR Antibody (HY-P83513) at 1/50dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • MTHFR Antibody (YA3258) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling MTHFR with MTHFR Antibody (HY-P83513) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MTHFR Antibody (HY-P83513) at 1/100dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • MTHFR Antibody (YA3258) Product Image WB
  • MTHFR Antibody (YA3258) Product Image ICC
  • MTHFR Antibody (YA3258) Product Image ICC