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Olfactomedin 4 Antibody (YA2288)

(Synonyms: GC1; GW112; hGC 1; hOLfD; olfactomedin 4; OlfD; OLFM4; OLM4)
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Based on 1 Customer Validation

Olfactomedin 4 Antibody (YA2288) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Olfactomedin 4.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Olfactomedin 4 Antibody (YA2288)
  • Olfactomedin 4 Antibody (YA2288) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:40

Product Details

Description

Olfactomedin 4 Antibody (YA2288) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Olfactomedin 4.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 54 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 57 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human OLFM4 aa100-157.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104648

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Olfactomedin 4 Antibody (YA2288)
    Western blot analysis was performed on extracts from SK-BR-3 (lane 1, 15 μg), MDA-MB-468 (lane 2, 15 μg), and LNcap (lane 3, 15 μg) using Olfactomedin 4 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Olfactomedin 4 may promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. May play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin

  • Subcellular Localization

    Secreted, extracellular space; Mitochondrion

  • Expression


    Tissue_specificity:Expressed during myeloid lineage development. Much higher expression in bone marrow neutrophils than in peripheral blood neutrophils (at protein level) . Strongly expressed in the prostate, small intestine and colon and moderately expressed in the bone marrow and stomach. Overexpressed in some pancreatic cancer tissues

    Induction:By retinoic acid. This induction requires functional NFKB pathway

  • Subunit

    Homomultimer; disulfide-linked. Interacts with NDUFA13. Interacts with cell surface lectins (locutions ricinus communis agglutinin I, concanavalin-A and wheat germ agglutinin) and cadherin

  • SwissProt ID

    Q6UX06

  • Gene ID
    10562 [NCBI]
  • Synonyms

    GC1; GW112; hGC 1; hOLfD; olfactomedin 4; OlfD; OLFM4; OLM4

  • Research Field

    Cancer

Olfactomedin 4 Antibody (YA2288) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from SK-BR-3 (lane 1, 15 μg), MDA-MB-468 (lane 2, 15 μg), and LNcap (lane 3, 15 μg) using Olfactomedin 4 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Olfactomedin 4 Antibody (YA2288) Product Image
    1/16
  • Experimental Validation Results for Olfactomedin 4 Antibody (YA2288)
  • Olfactomedin 4 Antibody (YA2288) Product Image
  • Experimental Validation Results for Olfactomedin 4 Antibody (YA2288)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from SK-BR-3 (lane 1, 15 μg), MDA-MB-468 (lane 2, 15 μg), and LNcap (lane 3, 15 μg) using Olfactomedin 4 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Olfactomedin 4 Antibody (YA2288)
  • Olfactomedin 4 Antibody (YA2288) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from SK-BR-3 (lane 1, 15 μg), MDA-MB-468 (lane 2, 15 μg), and LNcap (lane 3, 15 μg) using Olfactomedin 4 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Olfactomedin 4 Antibody (YA2288) Product Image WB