RNF213 Antibody (YA9385)
(Synonyms: ALO17, C17orf27, KIAA1554, KIAA1618, MYSTR, RNF213, E3 ubiquitin-protein ligase RNF213, ALK lymphoma oligomerization partner on chromosome 17, E3 ubiquitin-lipopolysaccharide ligase RNF213, Mysterin, RING finger protein 213)
Based on 1 Customer Validation
RNF213 Antibody (YA9385) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to RNF213.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, IP, ELISA
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Reactivity :
human
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Formulation:
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IP
IP: Immunoprecipitation
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:500-1000 | 1-2μg per 100-500μg Total protein | 1:50-3000 |
Product Details
RNF213 Antibody (YA9385) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to RNF213.
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Host Mouse
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Species Reactivityhuman
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Calculated Molecular Weight Predicted band size: 591 kDa
A synthesized peptide derived from human RNF213.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at 2-8°C for 1 year, do not freeze.
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Shipping
Shipping with blue ice.
Background
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Function
RNF213 is an Atypical E3 ubiquitin ligase that can catalyze ubiquitination of both proteins and lipids, and which is involved in various processes, such as lipid metabolism, angiogenesis and cell-autonomous immunity. Acts as a key immune sensor by catalyzing ubiquitination of the lipid A moiety of bacterial lipopolysaccharide (LPS) via its RZ-type zinc-finger: restricts the proliferation of cytosolic bacteria, such as Salmonella, by generating the bacterial ubiquitin coat through the ubiquitination of LPS. Also acts indirectly by mediating the recruitment of the LUBAC complex, which conjugates linear polyubiquitin chains. Ubiquitination of LPS triggers cell-autonomous immunity, such as antibacterial autophagy, leading to degradation of the microbial invader. Also functions as an intracellular sensor for ISGylated proteins: upon type I interferon stimulation, undergoes ISGylation and oligomerizes on lipid droplets to form a binding platform for ISG15-modified substrate proteins, thereby acting as a broad-spectrum antimicrobial effector. Restricts intracellular bacterial pathogens, such as Listeria monocytogenes, in an ISG15-dependent manner by decorating the bacterial surface with ubiquitin to promote autophagic clearance. Involved in lipid metabolism by regulating fat storage and lipid droplet formation; act by inhibiting the lipolytic process. Also regulates lipotoxicity by inhibiting desaturation of fatty acids. Also acts as an E3 ubiquitin-protein ligase via its RING-type zinc finger: mediates 'Lys-63'-linked ubiquitination of target proteins. Involved in the non-canonical Wnt signaling pathway in vascular development: acts by mediating ubiquitination and degradation of FLNA and NFATC2 downstream of RSPO3, leading to inhibit the non-canonical Wnt signaling pathway and promoting vessel regression. Also has ATPase activity; ATPase activity is required for ubiquitination of LPS[1][2][3][4][5][6][7][8][9][10][11].
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Subcellular Localization
Cytoplasm, cytosol; Lipid droplet
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Expression
Tissue_Specificity: Widely expressed (at protein level).
Induction: Down-regulated by let-7c miRNA, which binds to the 3'-UTR transcript of RNF213. Induced by pro-inflammatory cytokines. -
Isoforms & Post-Translational Modification
RNF213 has 4 isoforms, Q63HN8-3: amino acid length is 5207, molecular weight is 591407 Da (predicted); Q63HN8-4: amino acid length is 5256, molecular weight is 596486 Da (predicted); Q63HN8-5: amino acid length is 1063, molecular weight is 118436 Da (predicted); Q63HN8-6: amino acid length is 557, molecular weight is 64260 Da (predicted).Autoubiquitinated
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Subunit
Monomer (By similarity).
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SwissProt ID
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Synonyms
ALO17, C17orf27, KIAA1554, KIAA1618, MYSTR, RNF213, E3 ubiquitin-protein ligase RNF213, ALK lymphoma oligomerization partner on chromosome 17, E3 ubiquitin-lipopolysaccharide ligase RNF213, Mysterin, RING finger protein 213
Documentation
[1]. Liu W, et al. Identification of RNF213 as a susceptibility gene for moyamoya disease and its possible role in vascular development. PLoS One. 2011;6(7):e22542. [Content Brief]
[2]. Kobayashi H, et al. Biochemical and Functional Characterization of RNF213 (Mysterin) R4810K, a Susceptibility Mutation of Moyamoya Disease, in Angiogenesis In Vitro and In Vivo. J Am Heart Assoc. 2015 Jun 30;4(7):. [Content Brief]
[3]. Ohkubo K, et al. Moyamoya disease susceptibility gene RNF213 links inflammatory and angiogenic signals in endothelial cells. Sci Rep. 2015 Aug 17;5:13191. [Content Brief]
[4]. Scholz B, et al. Endothelial RSPO3 Controls Vascular Stability and Pruning through Non-canonical WNT/Ca(2+)/NFAT Signaling. Dev Cell. 2016 Jan 11;36(1):79-93. [Content Brief]
[5]. Sugihara M, et al. The AAA+ ATPase/ubiquitin ligase mysterin stabilizes cytoplasmic lipid droplets. J Cell Biol. 2019 Mar 4;218(3):949-960. [Content Brief]
[6]. Takeda M, et al. Moyamoya disease patient mutations in the RING domain of RNF213 reduce its ubiquitin ligase activity and enhance NFκB activation and apoptosis in an AAA+ domain-dependent manner. Biochem Biophys Res Commun. 2020 May 7;525(3):668-674. [Content Brief]
[7]. Otten EG, et al. Ubiquitylation of lipopolysaccharide by RNF213 during bacterial infection. Nature. 2021 Jun;594(7861):111-116. [Content Brief]
[8]. Crespillo-Casado A, et al. Recognition of phylogenetically diverse pathogens through enzymatically amplified recruitment of RNF213. EMBO Rep. 2024 Nov;25(11):4979-5005. [Content Brief]
[9]. Thery F, et al. Ring finger protein 213 assembles into a sensor for ISGylated proteins with antimicrobial activity. Nat Commun. 2021 Oct 1;12(1):5772. [Content Brief]
[10]. Piccolis M, et al. Probing the Global Cellular Responses to Lipotoxicity Caused by Saturated Fatty Acids. Mol Cell. 2019 Apr 4;74(1):32-44.e8. [Content Brief]
[11]. Habu T, et al. UBC13 is an RNF213-associated E2 ubiquitin-conjugating enzyme, and Lysine 63-linked ubiquitination by the RNF213-UBC13 axis is responsible for angiogenic activity. FASEB Bioadv. 2021 Apr;3(4):243-258. [Content Brief]
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