REA Antibody (YA1848)(PBS only)

Cat. No.: HY-P82103A: Data Sheet User Guide for Antibodies
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REA Antibody (YA1848) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to REA.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

REA Antibody (YA1848) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to REA.

Host

Rabbit

Molecular Weight

Predicted band size: 33 kDa;

Observed band size: 33 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q99623

Gene ID

11331 [NCBI]

Immunogen

A synthetic peptide of human REA

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Protein with pleiotropic attributes mediated in a cell-compartment- and tissue-specific manner, which include the plasma membrane-associated cell signaling functions, mitochondrial chaperone, and transcriptional co-regulator of transcription factors and sex steroid hormones in the nucleus; In the mitochondria, together with PHB, forms large ring complexes (prohibitin complexes) in the inner mitochondrial membrane (IMM) and functions as a chaperone protein that stabilizes mitochondrial respiratory enzymes and maintains mitochondrial integrity in the IMM, which is required for mitochondrial morphogenesis, neuronal survival, and normal lifespan (Probable). The prohibitin complex, with DNAJC19, regulates cardiolipin remodeling and the protein turnover of OMA1 in a cardiolipin-binding manner (By similarity). Also regulates cytochrome-c oxidase assembly (COX) and mitochondrial respiration (PubMed:11302691, PubMed:20959514). Binding to sphingoid 1-phosphate (SPP) modulates its regulator activity (PubMed:11302691, PubMed:20959514). Has a key role of mitophagy receptor involved in targeting mitochondria for autophagic degradation (PubMed:28017329). Involved in mitochondrial-mediated antiviral innate immunity, activates RIG-I-mediated signal transduction and production of IFNB1 and pro-inflammatory cytokine IL6 (PubMed:31522117); In the nucleus, serves as transcriptional co-regulator (Probable). Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity (By similarity); In the plasma membrane, is involved in IGFBP6-induced cell migration (PubMed:24003225). Cooperates with CD86 to mediate CD86-signaling in B lymphocytes that regulates the level of IgG1 produced through the activation of distal signaling intermediates. Upon CD40 engagement, required to activate NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity); (Microbial infection) Involved in human enterovirus 71/EV-71 infection by enhancing the autophagy mechanism during the infection

Subcellular Localization：Mitochondrion inner membrane; Cytoplasm; Nucleus; Cell membrane; Mitochondrion inner membrane; Mitochondrion inner membrane

Expression：
Tissue_specificity:Expressed in myoblasts

Induction:Expression increases approximately 3-fold upon entry into G1 phase compared to other phases of the cell cycle. Also induced following inhibition of mitochondrial protein synthesis by thiamphenicol

Isoforms & Post-Translational Modification：Q99623 has 2 isomers: Q99623-1: 33296 Da (predicted); Q99623-2: 29044 Da (predicted).
Phosphorylated. Tyrosine phosphorylation is indirectly stimulated by IGFBP6

Subunit：The mitochondrial prohibitin complex consists of two subunits (PHB1 and PHB2), assembled into a membrane-associated ring-shaped supercomplex of approximately 1 mDa (PubMed:20959514, PubMed:28017329, PubMed:31522117). Interacts with ESR1, HDAC1 and HDAC5 (By similarity). Interacts with ZNF703 (PubMed:21328542). Interacts with STOML2 (PubMed:21746876). Interacts with ARFGEF3 (PubMed:19496786). Interacts with SPHK2. Interacts with COX4I1; the interaction associates PHB2 with COX (By similarity). Interacts with MAP1LC3B (membrane-bound form LC3-II); the interaction is direct and upon mitochondrial depolarization and proteasome-dependent outer membrane rupture (PubMed:28017329). Interacts with IGFBP6 (via C-terminal domain) (PubMed:24003225). Interacts with CLPB (PubMed:31522117). Interacts with CD86 (via cytoplasmic domain); the interactions increases after priming with CD40 (By similarity). Interacts with AFG3L2 (By similarity). Interacts with DNAJC19 (By similarity). Interacts with AKT2; this interaction may be important for myogenic differentiation (By similarity)

Research Field

Signal Transduction

Synonyms

BAP; Bap37; BCAP 37; D prohibitin; p22; Phb2; PNAS 141; Prohibitin 2

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/mkn-45-cell.html [Content Brief]