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Cyclin E1 Antibody (YA483)

Customer Review

Based on 1 Customer Validation

Cyclin E1 Antibody (YA483) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cyclin E1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for Cyclin E1 Antibody (YA483)
  • Experimental Validation Results for Cyclin E1 Antibody (YA483)
  • Experimental Validation Results for Cyclin E1 Antibody (YA483)
  • Experimental Validation Results for Cyclin E1 Antibody (YA483)
  • Experimental Validation Results for Cyclin E1 Antibody (YA483)
  • Experimental Validation Results for Cyclin E1 Antibody (YA483)
  • Experimental Validation Results for Cyclin E1 Antibody (YA483)
  • Experimental Validation Results for Cyclin E1 Antibody (YA483)
  • Experimental Validation Results for Cyclin E1 Antibody (YA483)
  • Cyclin E1 Antibody (YA483) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:200 1:50-1:200

Product Details

Description

Cyclin E1 Antibody (YA483) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cyclin E1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 47 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 47 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Cyclin E1.AA range:361-410.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102303

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cyclin E1 Antibody (YA483)
    Western blot analysis of extracts from Hela(lane 2(20μg) , NIH/3T3 (lane 3(20μg) ,MCF-7(lane 4(20μg)and K562( lane 5(20μg) using Cyclin E1 Antibody (HY-P80100). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Cyclin E1 Antibody (YA483)
    Immunohistochemical analysis of paraffin-embedded human Testis tissue using Cyclin E1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80100, 1:2000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Cyclin E1 Antibody (YA483)
    Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using Cyclin E1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80100, 1:2000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Cyclin E1 Antibody (YA483)
    Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using Cyclin E1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80100, 1:2000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Cyclin E1 Antibody (YA483)
    Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using Cyclin E1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80100, 1:2000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Cyclin E1 Antibody (YA483)
    Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using Cyclin E1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80100, 1:2000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Cyclin E1 Antibody (YA483)
    Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using Cyclin E1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80100, 1:2000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Cyclin E1 Antibody (YA483)
    Immunocytochemistry analysis of HeLa cells labeling Cyclin E1 with Cyclin E1 Antibody (HY-P80100) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cyclin E1 Antibody (HY-P80100) at 1/50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Cyclin E1 Antibody (YA483)
    Immunocytochemistry analysis of HeLa cells labeling Cyclin E1 with Cyclin E1 Antibody (HY-P80100) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cyclin E1 Antibody (HY-P80100) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Essential for the control of the cell cycle at the G1/S (start) transition

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:It is highly expressed in the testes and placenta. Its expression level is lower in bronchial epithelial cells.

  • Subunit

    Interacts with CDK2 protein kinase to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex (PubMed:15660127). Found in a complex with CDK2, CABLES1 and CCNA1 (By similarity). Part of a complex consisting of UHRF2, CDK2 and CCNE1 (PubMed:15178429). Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation (PubMed:21952639). Interacts with INCA1 (PubMed:21540187)

  • SwissProt ID

    P24864

  • Gene ID
    898 [NCBI]
  • Research Field

    Cell Biology

Cyclin E1 Antibody (YA483) Related Classifications

100 mg

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Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    Cyclin E1 Antibody (YA483) purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20μg) , NIH/3T3 (lane 3(20μg) ,MCF-7(lane 4(20μg)and K562( lane 5(20μg) using Cyclin E1 Antibody (HY-P80100). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • 1/16

    Cyclin E1 Antibody (YA483) purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Testis tissue using Cyclin E1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80100, 1:2000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    Cyclin E1 Antibody (YA483) purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using Cyclin E1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80100, 1:2000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    Cyclin E1 Antibody (YA483) purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using Cyclin E1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80100, 1:2000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    Cyclin E1 Antibody (YA483) purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using Cyclin E1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80100, 1:2000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    Cyclin E1 Antibody (YA483) purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using Cyclin E1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80100, 1:2000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    Cyclin E1 Antibody (YA483) purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using Cyclin E1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80100, 1:2000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    Cyclin E1 Antibody (YA483) purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling Cyclin E1 with Cyclin E1 Antibody (HY-P80100) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cyclin E1 Antibody (HY-P80100) at 1/50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    Cyclin E1 Antibody (YA483) purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling Cyclin E1 with Cyclin E1 Antibody (HY-P80100) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cyclin E1 Antibody (HY-P80100) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Cyclin E1 Antibody (YA483) Product Image
    1/16
  • Experimental Validation Results for Cyclin E1 Antibody (YA483)
  • Experimental Validation Results for Cyclin E1 Antibody (YA483)
  • Experimental Validation Results for Cyclin E1 Antibody (YA483)
  • Experimental Validation Results for Cyclin E1 Antibody (YA483)
  • Experimental Validation Results for Cyclin E1 Antibody (YA483)
  • Experimental Validation Results for Cyclin E1 Antibody (YA483)
  • Experimental Validation Results for Cyclin E1 Antibody (YA483)
  • Experimental Validation Results for Cyclin E1 Antibody (YA483)
  • Experimental Validation Results for Cyclin E1 Antibody (YA483)
  • Cyclin E1 Antibody (YA483) Product Image
  • Cyclin E1 Antibody (YA483) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20μg) , NIH/3T3 (lane 3(20μg) ,MCF-7(lane 4(20μg)and K562( lane 5(20μg) using Cyclin E1 Antibody (HY-P80100). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • Cyclin E1 Antibody (YA483) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Testis tissue using Cyclin E1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80100, 1:2000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Cyclin E1 Antibody (YA483) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using Cyclin E1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80100, 1:2000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Cyclin E1 Antibody (YA483) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using Cyclin E1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80100, 1:2000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Cyclin E1 Antibody (YA483) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using Cyclin E1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80100, 1:2000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Cyclin E1 Antibody (YA483) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using Cyclin E1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80100, 1:2000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Cyclin E1 Antibody (YA483) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using Cyclin E1 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80100, 1:2000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Cyclin E1 Antibody (YA483) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling Cyclin E1 with Cyclin E1 Antibody (HY-P80100) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cyclin E1 Antibody (HY-P80100) at 1/50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Cyclin E1 Antibody (YA483) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling Cyclin E1 with Cyclin E1 Antibody (HY-P80100) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Cyclin E1 Antibody (HY-P80100) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Cyclin E1 Antibody (YA483) Product Image WB
  • Cyclin E1 Antibody (YA483) Product Image IHC-P
  • Cyclin E1 Antibody (YA483) Product Image IHC-P
  • Cyclin E1 Antibody (YA483) Product Image IHC-P
  • Cyclin E1 Antibody (YA483) Product Image IHC-P
  • Cyclin E1 Antibody (YA483) Product Image IHC-P
  • Cyclin E1 Antibody (YA483) Product Image IHC-P
  • Cyclin E1 Antibody (YA483) Product Image ICC
  • Cyclin E1 Antibody (YA483) Product Image ICC