TRIM39 (RNF23) Antibody (YA8219)

Cat. No.: HY-P88535: Data Sheet User Guide for Antibodies
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TRIM39 (RNF23) Antibody (YA8219) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TRIM39 (RNF23).

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

TRIM39 (RNF23) Antibody (YA8219) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TRIM39 (RNF23).

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 56.2 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9HCM9

Immunogen

Human recombinant protein fragment corresponding to amino acids 144-378 of human TRIM39 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：E3 ubiquitin-protein ligase (PubMed:22529100). May facilitate apoptosis by inhibiting APC/C-Cdh1-mediated poly-ubiquitination and subsequent proteasome-mediated degradation of the pro-apoptotic protein MOAP1 (PubMed:19100260, PubMed:22529100). Regulates the G1/S transition of the cell cycle and DNA damage-induced G2 arrest by stabilizing CDKN1A/p21 (PubMed:23213251). Positively regulates CDKN1A/p21 stability by competing with DTL for CDKN1A/p21 binding, therefore disrupting DCX(DTL) E3 ubiquitin ligase complex-mediated CDKN1A/p21 ubiquitination and degradation (PubMed:23213251)|Regulates the G1/S transition of the cell cycle and DNA damage-induced G2 arrest by stabilizing CDKN1A/p21 (PubMed:23213251). Positively regulates CDKN1A/p21 stability by competing with DTL for CDKN1A/p21 binding, therefore disrupting DCX(DTL) E3 ubiquitin ligase complex-mediated CDKN1A/p21 ubiquitination and degradation (PubMed:23213251). Negatively regulates the canonical NF-kappa-B signaling pathway via stabilization of CACTIN in an ubiquitination-independent manner (PubMed:26363554)

Subcellular Localization：Cytoplasm, cytosol,Mitochondrion,Nucleus,Nucleus

Expression：
Tissue_Specificity: Ubiquitous; highly expressed in brain, heart, kidney, liver, skeletal muscle, spleen and testis

Isoforms & Post-Translational Modification：Q9HCM9 has two isomers: Q9HCM9-1: 59690 Da (predicted); Q9HCM9-2: 56374 Da (predicted).
Autoubiquitinated

Subunit：Isoform 1 interacts with MOAP1 (PubMed:19100260)

Synonyms

RNF23; TFP; TRIM39B

Documentation

User Guide for Antibodies (1077 KB)