CYP3A5 Antibody (YA9013)
(Synonyms: Cytochrome P450 3A5, CYPIIIA5, Cytochrome P450-PCN3, CYP3A5)
Based on 1 Customer Validation
CYP3A5 Antibody (YA9013) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CYP3A5.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, ICC/IF, IF-Tissue, IHC-P, IP
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Reactivity :
human
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Formulation:
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|---|
| Dilution Ratio | 1:500-1000 | 1:50-500 | 1:50-500 | 1:100-500 | 1-2μg per 100-500μg Total protein |
Product Details
CYP3A5 Antibody (YA9013) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CYP3A5.
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Host Mouse
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Species Reactivityhuman
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Calculated Molecular Weight Predicted band size: 57 kDa
OMIM: 605325
A synthesized peptide derived from human CYP3A5.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at 2-8°C for 1 year, do not freeze.
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Shipping
Shipping with blue ice.
Background
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Function
CYP3A5 is an A cytochrome P450 monooxygenase involved in the metabolism of steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of catechol estrogens from 17beta-estradiol (E2) and estrone (E1), namely 2-hydroxy E1 and E2. Catalyzes 6beta-hydroxylation of the steroid hormones testosterone, progesterone, and androstenedione. Catalyzes the oxidative conversion of all-trans-retinol to all-trans-retinal, a rate-limiting step for the biosynthesis of all-trans-retinoic acid (atRA). Further metabolizes all trans-retinoic acid (atRA) to 4-hydroxyretinoate and may play a role in hepatic atRA clearance. Also involved in the oxidative metabolism of xenobiotics, including calcium channel blocking drug nifedipine and immunosuppressive drug cyclosporine[1][2][3][4].
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Subcellular Localization
Endoplasmic reticulum membrane; Microsome membrane
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Expression
Induction: By glucocorticoids, such as dexamethesone. -
Isoforms & Post-Translational Modification
CYP3A5 has 2 isoforms, P20815-1: amino acid length is 502, molecular weight is 57109 Da (predicted); P20815-2: amino acid length is 140, molecular weight is 15846 Da (predicted).
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SwissProt ID
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Synonyms
Cytochrome P450 3A5, CYPIIIA5, Cytochrome P450-PCN3, CYP3A5
Documentation
References
[1]. Chen H, et al. Biosynthesis of all-trans-retinoic acid from all-trans-retinol: catalysis of all-trans-retinol oxidation by human P-450 cytochromes. Drug Metab Dispos. 2000 Mar;28(3):315-22. [Content Brief]
[2]. Marill J, et al. Identification of human cytochrome P450s involved in the formation of all-trans-retinoic acid principal metabolites. Mol Pharmacol. 2000 Dec;58(6):1341-8. [Content Brief]
[3]. Lee AJ, et al. Characterization of the oxidative metabolites of 17beta-estradiol and estrone formed by 15 selectively expressed human cytochrome p450 isoforms. Endocrinology. 2003 Aug;144(8):3382-98. [Content Brief]
[4]. Aoyama T, et al. Cytochrome P-450 hPCN3, a novel cytochrome P-450 IIIA gene product that is differentially expressed in adult human liver. cDNA and deduced amino acid sequence and distinct specificities of cDNA-expressed hPCN1 and hPCN3 for the metabolism of steroid hormones and cyclosporine. J Biol Chem. 1989 Jun 25;264(18):10388-95. [Content Brief]
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