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CRABP1 Antibody (YA2467)

(Synonyms: CRABP; Crabp1; CRABPI; RBP5)
Customer Review

Based on 1 Customer Validation

CRABP1 Antibody (YA2467) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CRABP1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CRABP1 Antibody (YA2467)
  • CRABP1 Antibody (YA2467) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50

Product Details

Description

CRABP1 Antibody (YA2467) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CRABP1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 16 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 16 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human CRABP1 aa100-137/137.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104039

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CRABP1 Antibody (YA2467)
    Western blot analysis of extracts from Caco-2 (lane 2, 20ug), PC-3 (lane 3, 20ug), SK-OV-3 (lane 4, 20ug) and THP-1 (lane 5, 20ug), MCF-7 (lane 6, 20ug), NIH/3T3 (lane 7, 20ug) using CRABP1 Antibody (HY-P82722). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    CRABP1 is a Cytosolic CRABPs may regulate the access of retinoic acid to the nuclear retinoic acid receptors

  • Subcellular Localization

    Cytoplasm

  • SwissProt ID

    P29762

  • Gene ID
    1381 [NCBI]
  • Synonyms

    CRABP; Crabp1; CRABPI; RBP5

  • Research Field

    -

CRABP1 Antibody (YA2467) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Caco-2 (lane 2, 20ug), PC-3 (lane 3, 20ug), SK-OV-3 (lane 4, 20ug) and THP-1 (lane 5, 20ug), MCF-7 (lane 6, 20ug), NIH/3T3 (lane 7, 20ug) using CRABP1 Antibody (HY-P82722). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • CRABP1 Antibody (YA2467) Product Image
    1/16
  • Experimental Validation Results for CRABP1 Antibody (YA2467)
  • CRABP1 Antibody (YA2467) Product Image
  • Experimental Validation Results for CRABP1 Antibody (YA2467)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Caco-2 (lane 2, 20ug), PC-3 (lane 3, 20ug), SK-OV-3 (lane 4, 20ug) and THP-1 (lane 5, 20ug), MCF-7 (lane 6, 20ug), NIH/3T3 (lane 7, 20ug) using CRABP1 Antibody (HY-P82722). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CRABP1 Antibody (YA2467)
  • CRABP1 Antibody (YA2467) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Caco-2 (lane 2, 20ug), PC-3 (lane 3, 20ug), SK-OV-3 (lane 4, 20ug) and THP-1 (lane 5, 20ug), MCF-7 (lane 6, 20ug), NIH/3T3 (lane 7, 20ug) using CRABP1 Antibody (HY-P82722). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • CRABP1 Antibody (YA2467) Product Image WB