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PRMT1 Antibody (YA686)

(Synonyms: ANM 1; ANM1; ANM1_HUMAN; complete cds; HCP 1; HCP1; Heterogeneous nuclear ribonucleoprotein methyltransferase 1 like 2; Heterogeneous nuclear ribonucleoproteins methyltransferase like 2; Heterogeneous nuclear ribonucleoproteins methyltransferase like2; Hi)
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Based on 1 Customer Validation

PRMT1 Antibody (YA686) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to PRMT1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PRMT1 Antibody (YA686)
  • PRMT1 Antibody (YA686) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

PRMT1 Antibody (YA686) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to PRMT1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 42 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 42 kDa
Species Reactivity Database

Entrez Gene: 3276 Human

SwissProt: Q99873 Human

Immunogen

Synthetic peptide corresponding to Human PRMT1.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102242

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PRMT1 Antibody (YA686)
    Western blot analysis of extracts from Hela(lane 2(20ug) , A549(lane 3(20ug) and HEK293(lane 4(20ug) using PRMT1 Antibody (HY-P80874) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Protein arginine methyltransferase 1 (PRMT1) is the primary type I PRMT responsible for asymmetric dimethylation of arginine residues on histone and non-histone proteins[1][2]. Mechanistically, PRMT1 regulates gene transcription, RNA processing, DNA replication, and signal transduction pathways critical for cell proliferation and survival[3][2]. PRMT1 activity influences type I interferon signaling and modulates anti-tumor immunity, particularly in lung adenocarcinoma, affecting proliferation, migration, and invasion[4]. In disease models, PRMT1 dysregulation contributes to persistence of cancer cells under EGFR- and KRAS-targeted therapies, with knockdown enhancing drug efficacy and prolonging tumor regression in xenografts[5]. Compared with related isoforms, PRMT1 exhibits distinct substrate specificity and nuclear localization, unlike PRMT3 which is primarily cytoplasmic, or PRMT8 which has brain-specific expression[6][7]. Functionally, PRMT1 shows sensitivity to ionic strength, temperature, and pH, allowing regulation under cellular stress conditions[2]. Experimental applications have demonstrated that inhibition of PRMT1, alone or combined with PRMT5 blockade, sensitizes cancer cells to DNA damage and PARP inhibitors, highlighting its therapeutic potential[8][9]. Collectively, PRMT1 is a key epigenetic regulator, distinct from other PRMT isoforms, and serves as a critical target in cancer biology and therapeutic design[10][5].

  • Subcellular Localization

    Nucleus; Nucleus, nucleoplasm; Cytoplasm; Cytoplasm, cytosol; Lysosome membrane

  • Expression


    Tissue_specificity:Widely expressed (PubMed:11097842) . Highly expressed in colorectal cancer cells (protein level) (PubMed:28040436) . Highly expressed in colorectal cancer tissues compared to wild-type colon tissue (protein level) (PubMed:28040436) . Highly expressed in colorectal cancer tissues compared to wild-type colon tissue (PubMed:28040436) .

  • Isoforms & Post-Translational Modification

    Q99873 has 4 isomers: Q99873-1: 42462 Da (predicted); Q99873-2: 39929 Da (predicted); Q99873-3: 40548 Da (predicted); Q99873-5: 32693 Da (predicted).
    Polyubiquitinated at Lys-145 by the SCF(FBXL17) complex, leading to its subsequent degradation (By similarity). Ubiquitination is regulated by acetylation at Lys-228 and Lys-233 (By similarity). Polyubiquitinated by E3 ubiquitin-protein ligase TRIM48, leading to suppression of MAP3K5/ASK1 methylation and subsequent MAP3K5 activation (PubMed:29186683);Acetylation at Lys-228 and Lys-233 regulates ubiquitination by the SCF(FBXL17) complex. Acetylated at Lys-233 by p300/EP300. Deacetylated at Lys-228 and Lys-233 by SIRT1

  • Subunit

    Homodimer (PubMed:16879614, PubMed:26876602). Homooctamer; individual homodimers associates to form a homooctamer (PubMed:26876602).

  • SwissProt ID

    Q99873

  • Gene ID
    3276 [NCBI]
  • Synonyms

    ANM 1; ANM1; ANM1_HUMAN; complete cds; HCP 1; HCP1; Heterogeneous nuclear ribonucleoprotein methyltransferase 1 like 2; Heterogeneous nuclear ribonucleoproteins methyltransferase like 2; Heterogeneous nuclear ribonucleoproteins methyltransferase like2; Hi

  • Research Field

    Cell Biology

[1]. Wang YC, et al. Identification, chromosomal arrangements and expression analyses of the evolutionarily conserved prmt1 gene in chicken in comparison with its vertebrate paralogue prmt8. PLoS One. 2017 Sep 21;12(9):e0185042. [Content Brief]

[2]. Lowe TL, et al. Human protein arginine methyltransferases (PRMTs) can be optimally active under nonphysiological conditions. J Biol Chem. 2022 Sep;298(9):102290. [Content Brief]

[3]. Yoshimatsu M, et al. Dysregulation of PRMT1 and PRMT6, Type I arginine methyltransferases, is involved in various types of human cancers. Int J Cancer. 2011 Feb 1;128(3):562-73. [Content Brief]

[4]. Wang J, et al. Pan-cancer analysis identifies protein arginine methyltransferases PRMT1 and PRMT5 and their related signatures as markers associated with prognosis, immune profile, and therapeutic response in lung adenocarcinoma. Heliyon. 2023 Nov 27;9(12):e22088. [Content Brief]

[5]. Sun X, et al. Targeting PRMT1 Reduces Cancer Persistence and Tumor Relapse in EGFR- and KRAS-Mutant Lung Cancer. Cancer Res Commun. 2025 Jan 1;5(1):119-127. [Content Brief]

[6]. Tang J, et al. PRMT 3, a type I protein arginine N-methyltransferase that differs from PRMT1 in its oligomerization, subcellular localization, substrate specificity, and regulation. J Biol Chem. 1998 Jul 3;273(27):16935-45. [Content Brief]

[7]. Lin YL, et al. The critical role of protein arginine methyltransferase prmt8 in zebrafish embryonic and neural development is non-redundant with its paralogue prmt1. PLoS One. 2013;8(3):e55221. [Content Brief]

[8]. Zhang Y, et al. Repression of PRMT activities sensitize human homologous recombination-proficient ovarian and breast cancer cells to PARP inhibitor treatment. Elife. 2026 Feb 3;13:RP99225. [Content Brief]

[9]. Gao G, et al. PRMT1 loss sensitizes cells to PRMT5 inhibition. Nucleic Acids Res. 2019 Jun 4;47(10):5038-5048. [Content Brief]

[10]. Li M, et al. The arginine methyltransferase PRMT5 and PRMT1 distinctly regulate the degradation of anti-apoptotic protein CFLARL in human lung cancer cells. J Exp Clin Cancer Res. 2019 Feb 8;38(1):64. [Content Brief]

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PRMT1 Antibody (YA686) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) , A549(lane 3(20ug) and HEK293(lane 4(20ug) using PRMT1 Antibody (HY-P80874) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PRMT1 Antibody (YA686) Product Image
    1/16
  • Experimental Validation Results for PRMT1 Antibody (YA686)
  • PRMT1 Antibody (YA686) Product Image
  • Experimental Validation Results for PRMT1 Antibody (YA686)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) , A549(lane 3(20ug) and HEK293(lane 4(20ug) using PRMT1 Antibody (HY-P80874) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PRMT1 Antibody (YA686)
  • PRMT1 Antibody (YA686) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug) , A549(lane 3(20ug) and HEK293(lane 4(20ug) using PRMT1 Antibody (HY-P80874) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PRMT1 Antibody (YA686) Product Image WB