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Serotonin Transporter Antibody (YA1638)

(Synonyms: SLC6A4; 5HTT; 5HT transporter; 5-HTT; 5-HTTLPR; OCD1; SERT; Serotonin transporter; HSERT; SERT1)
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Based on 1 Customer Validation

Serotonin Transporter Antibody (YA1638) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Serotonin Transporter.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Serotonin Transporter Antibody (YA1638)
  • Serotonin Transporter Antibody (YA1638) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Serotonin Transporter Antibody (YA1638) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Serotonin Transporter.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 70 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 70 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Serotonin transporter aa50-100/630.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103990

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Serotonin Transporter Antibody (YA1638)
    Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 20 μg), and Mouse brain (lane 2, 20 μg) using Serotonin Transporter Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Serotonin Transporters (SERTs) are integral membrane proteins that transport serotonin from synaptic spaces into presynaptic neurons. SERTs function by reuptaking serotonin in the synaptic cleft, effectively terminating the function of serotonin and halting neuronal transmission. Serotonin reuptake is a critical process to prevent overstimulation of nerves. Serotonin transporter (SERT) regulates extracellular levels of serotonin (5-hydroxytryptamine, 5HT) in the brain by transporting 5HT into neurons and glial cells. The human SERT (hSERT) is the primary target for drugs used in the treatment of emotional disorders, including depression. hSERT belongs to the solute carrier 6 family that includes a bacterial leucine transporter (LeuT), for which a high resolution crystal structure has become available.

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein; Endomembrane system; Multi-pass membrane protein; Endosome membrane; Multi-pass membrane protein; Synapse; Cell junction, focal adhesion; Cell projection, neuron projection

  • Expression


    Tissue_specificity:Expressed in platelets (at protein level)

  • Isoforms & Post-Translational Modification

    P31645 has 2 isomers: P31645-1: 70325 Da (predicted); P31645-2: 74979 Da (predicted).
    Phosphorylation at Thr-276 increases 5-HT uptake and is required for cGMP-mediated SERT regulation

  • Subunit

    Monomer or homooligomer (By similarity). Interacts with TGFB1I1 (PubMed:16803896). Interacts with SEC23A, SEC24C and PATJ.

  • SwissProt ID

    P31645

  • Gene ID
    6532 [NCBI]
  • Synonyms

    SLC6A4; 5HTT; 5HT transporter; 5-HTT; 5-HTTLPR; OCD1; SERT; Serotonin transporter; HSERT; SERT1

  • Research Field

    Neuroscience

Serotonin Transporter Antibody (YA1638) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 20 μg), and Mouse brain (lane 2, 20 μg) using Serotonin Transporter Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Serotonin Transporter Antibody (YA1638) Product Image
    1/16
  • Experimental Validation Results for Serotonin Transporter Antibody (YA1638)
  • Serotonin Transporter Antibody (YA1638) Product Image
  • Experimental Validation Results for Serotonin Transporter Antibody (YA1638)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 20 μg), and Mouse brain (lane 2, 20 μg) using Serotonin Transporter Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Serotonin Transporter Antibody (YA1638)
  • Serotonin Transporter Antibody (YA1638) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 20 μg), and Mouse brain (lane 2, 20 μg) using Serotonin Transporter Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Serotonin Transporter Antibody (YA1638) Product Image WB