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  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. Serotonin Transporter Antibody (YA1638)

Serotonin Transporter Antibody (YA1638)

Cat. No.: HY-P81893
COA
SDS
User Guide for Antibodies Technical Support

Serotonin Transporter Antibody (YA1638) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Serotonin Transporter.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

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Description

Serotonin Transporter Antibody (YA1638) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Serotonin Transporter.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Molecular Weight
Predicted band size: 70 kDa;
Observed band size: 70 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human Serotonin transporter aa50-100/630.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
Sensitivity Endogenous Purity Affinity Chromatography
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 20 μg), and Mouse brain (lane 2, 20 μg) using Serotonin Transporter Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background
Function：Serotonin transporter that cotransports serotonin witH2O2e Na(+) ion in exchange for one K(+) ion and possibly one proton in an overall electroneutral transport cycle. Transports serotonin across the plasma membrane from the extracellular compartment to the cytosol thus limiting serotonin intercellular signaling (PubMed:10407194, PubMed:12869649, PubMed:21730057, PubMed:27049939, PubMed:27756841, PubMed:34851672). Essential for serotonin homeostasis in the central nervous system. In the developing somatosensory cortex, acts in glutamatergic neurons to control serotonin uptake and its trophic functions accounting for proper spatial organization of cortical neurons and elaboration of sensory circuits. In the mature cortex, acts primarily in brainstem raphe neurons to mediate serotonin uptake from the synaptic cleft back into the pre-synaptic terminal thus terminating serotonin signaling at the synapse (By similarity). Modulates mucosal serotonin levels in the gastrointestinal tract tH2O2gh uptake and clearance of serotonin in enterocytes. Required for enteric neurogenesis and gastrointestinal reflexes (By similarity). Regulates blood serotonin levels by ensuring rapid high affinity uptake of serotonin from plasma to platelets, where it is further stored in dense granules via vesicular monoamine transporters and then released upon stimulation (PubMed:17506858, PubMed:18317590). Mechanistically, the transport cycle starts with an outward-open conformation having Na1(+) and Cl(-) sites occupied. The binding of a second extracellular Na2(+) ion and serotonin substrate leads to structural changes to outward-occluded to inward-occluded to inward-open, where the Na2(+) ion and serotonin are released into the cytosol. Binding of intracellular K(+) ion induces conformational transitions to inward-occluded to outward-open and completes the cycle by releasing K(+) possibly together with a proton bound to Asp-98 into the extracellular compartment. Na1(+) and Cl(-) ions remain bound tH2O2ghout the transport cycle (PubMed:10407194, PubMed:12869649, PubMed:21730057, PubMed:27049939, PubMed:27756841, PubMed:34851672). Additionally, displays serotonin-induced channel-like conductance for monovalent cations, mainly Na(+) ions. The channel activity is uncoupled from the transport cycle and may contribute to the membrane resting potential or excitability (By similarity)
Subcellular Localization：Cell membrane; Multi-pass membrane protein; Endomembrane system; Multi-pass membrane protein; Endosome membrane; Multi-pass membrane protein; Synapse; Cell junction, focal adhesion; Cell projection, neuron projection
Expression：
Tissue_specificity:Expressed in platelets (at protein level)
Isoforms & Post-Translational Modification：P31645 has 2 isomers: P31645-1: 70325 Da (predicted); P31645-2: 74979 Da (predicted).
Phosphorylation at Thr-276 increases 5-HT uptake and is required for cGMP-mediated SERT regulation
Subunit：Monomer or homooligomer (By similarity). Interacts with TGFB1I1 (PubMed:16803896). Interacts with SEC23A, SEC24C and PATJ.
Database

Entrez Gene: 6532 Human ; 15567 Mouse ; 25553 Rat

SwissProt: P31645 Human ; Q60857 Mouse ; P31652 Rat

OMIM: 103780 Human

Research Field

Neuroscience
Synonyms
SLC6A4; 5HTT; 5HT transporter; 5-HTT; 5-HTTLPR; OCD1; SERT; Serotonin transporter; HSERT; SERT1
Documentation
SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Serotonin Transporter Antibody (YA1638) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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