Transcription Factor AP 2 gamma Antibody (YA4399)

Cat. No.: HY-P84702: Data Sheet User Guide for Antibodies
FAQs
Technical Support

Transcription Factor AP 2 gamma Antibody (YA4399) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Transcription Factor AP 2 gamma.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Get quote

Synthetic products have potential research and development risk.

or Bulk Inquiry

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Transcription Factor AP 2 gamma Antibody (YA4399) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Transcription Factor AP 2 gamma.

Host

Mouse

Clonality

Monoclonal Antibody

Molecular Weight

Predicted band size: 49 kDa;

Observed band size: 50 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q92754

Gene ID

7022 [NCBI]

Immunogen

Purified recombinant fragment of human TFAP2C (AA: 341-450) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Sequence-specific DNA-binding transcription factor that interacts with cellular enhancer elements to regulate transcription of selected genes, and which plays a key role in early embryonic development (PubMed:11694877, PubMed:24413532). AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions (PubMed:11694877, PubMed:24413532). TFAP2C plays a key role in early embryonic development by regulating both inner cell mass (ICM) and trophectoderm differentiation (By similarity). At the 8-cell stage, during morula development, controls expression of cell-polarity genes (By similarity). Upon trophoblast commitment, binds to late trophectoderm genes in blastocysts together with CDX2, and later to extra-embryonic ectoderm genes together with SOX2 (By similarity). Binds to both closed and open chromatin with other transcription factors (By similarity). Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer (PubMed:24413532)

Subcellular Localization：Nucleus

Expression：
Induction:During retinoic acid-mediated differentiation

Isoforms & Post-Translational Modification：Q92754 has 2 isomers: Q92754-1: 49177 Da (predicted); Q92754-2: 31010 Da (predicted).
Sumoylated on Lys-10; which inhibits transcriptional activity

Subunit：Binds DNA as a dimer (PubMed:11694877). Can form homodimers or heterodimers with other AP-2 family members (PubMed:11694877). Interacts with WWOX (PubMed:15548692). Interacts with UBE2I (PubMed:12072434). Interacts with KCTD1; this interaction represses transcription activation (PubMed:19115315). Interacts with CITED2 (via C-terminus); the interaction stimulates TFAP2B-transcriptional activity (PubMed:11694877, PubMed:12586840). Interacts with CITED4 (PubMed:11744733). Interacts with MTA1 (PubMed:24413532)

Synonyms

ERF1; TFAP2G; hAP-2g; AP2-GAMMA

Documentation

User Guide for Antibodies (1077 KB)